Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ODI_RS01180 Genome accession   NZ_LT907988
Coordinates   245316..246752 (+) Length   478 a.a.
NCBI ID   WP_067753559.1    Uniprot ID   A0A1C3K1W1
Organism   Orrella dioscoreae isolate Orrdi1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 240316..251752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ODI_RS01165 (ODI_04045) - 241161..242321 (+) 1161 WP_067753567.1 DSD1 family PLP-dependent enzyme -
  ODI_RS01170 (ODI_04044) leuA 242382..244091 (-) 1710 WP_067753565.1 2-isopropylmalate synthase -
  ODI_RS01175 (ODI_04043) - 244440..245300 (-) 861 WP_067753562.1 hypothetical protein -
  ODI_RS01180 (ODI_04042) rpoN/rpoN1 245316..246752 (+) 1437 WP_067753559.1 RNA polymerase factor sigma-54 Machinery gene
  ODI_RS01185 (ODI_04041) - 246877..247149 (+) 273 WP_067753556.1 bacterioferritin-associated ferredoxin -
  ODI_RS01190 (ODI_04040) bfr 247523..248002 (+) 480 WP_067753553.1 bacterioferritin -
  ODI_RS01195 (ODI_04039) - 248103..248774 (-) 672 WP_067753550.1 hypothetical protein -
  ODI_RS01200 (ODI_04038) - 248840..250447 (-) 1608 WP_067753547.1 peptide chain release factor 3 -
  ODI_RS01205 (ODI_04037) - 250517..251182 (-) 666 WP_067753544.1 DUF799 domain-containing protein -
  ODI_RS01210 (ODI_04036) - 251179..251556 (-) 378 WP_067753647.1 DUF4810 domain-containing protein -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 52094.86 Da        Isoelectric Point: 4.8851

>NTDB_id=1148219 ODI_RS01180 WP_067753559.1 245316..246752(+) (rpoN/rpoN1) [Orrella dioscoreae isolate Orrdi1]
MTRHSLELRHSQHLTLTPQLQQSIRMLQLSALELEMEIAQALTENPMLEREGEPGGDSEGDAPAEGDAGDSGADYESATV
SLEDGMRGSGSSQNDDDDRAFPEAVRAETLREHLLGQLSLTRVSDRDAALVELLIDELDENGFLASSLEEILGWLPAELE
CDEDELHAALTLLRSFDPSGVGAFDVADCLVLQLRHPDPERFPEAADPQVLALARKLCAEHLQLLAGGSAAKLREALHCG
DEALRAAHSLILRLDPRPGRAWTVPAADYAVPDVIVHKTRKGWQAVLNTAVMPRLRINSVYAQVLASNRSGDHSALQSQL
QQARWMVRNVEQRFDTILRVSQAIVDRQSEFFSHGAGAMQPLILKDIAGELGLHESTISRATTQKFMLTPFGTMELKRFF
GAGVATEAGGSASATAVQAQIRQMVAEESAGKPLSDNQIMQMLADRGIVIARRTVAKYREALRIAPASVRKAQASLRT

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=1148219 ODI_RS01180 WP_067753559.1 245316..246752(+) (rpoN/rpoN1) [Orrella dioscoreae isolate Orrdi1]
ATGACGCGCCATTCCTTAGAACTGCGACACAGTCAGCACCTCACGCTGACACCGCAACTGCAGCAATCCATCCGGATGCT
GCAGCTGTCGGCGCTCGAGCTCGAAATGGAGATCGCGCAGGCGCTCACTGAAAACCCCATGCTCGAGCGCGAAGGCGAGC
CGGGCGGCGATTCCGAAGGCGATGCCCCCGCCGAGGGCGACGCGGGTGACAGCGGCGCGGATTACGAGTCCGCCACGGTA
TCGCTGGAAGACGGCATGCGCGGCAGCGGTTCCAGCCAGAACGATGACGACGACCGCGCCTTTCCCGAGGCGGTGCGCGC
CGAGACGCTGCGCGAGCACCTGCTCGGCCAGCTGTCGCTGACCCGCGTCAGCGACCGTGATGCGGCGCTGGTCGAGCTGC
TCATCGACGAACTGGACGAGAACGGCTTCCTGGCCTCTTCGCTGGAAGAGATCCTGGGCTGGCTGCCCGCCGAACTGGAG
TGCGACGAAGATGAGTTGCACGCCGCGCTCACGCTGCTGCGCTCCTTCGATCCCTCGGGGGTGGGGGCCTTCGACGTGGC
CGACTGCCTGGTGCTGCAATTGCGCCACCCCGACCCGGAGCGCTTCCCCGAGGCTGCCGATCCGCAGGTGCTGGCGCTGG
CCCGCAAGCTCTGTGCCGAGCATCTTCAGCTGCTGGCCGGTGGCAGCGCGGCCAAGCTGCGCGAGGCGCTGCACTGTGGC
GACGAGGCCCTGCGCGCGGCCCATTCGCTCATCCTGCGCCTGGATCCGCGTCCGGGCCGCGCGTGGACCGTGCCCGCGGC
CGATTACGCCGTGCCCGACGTCATCGTGCACAAGACGCGCAAAGGTTGGCAGGCCGTGCTGAACACCGCCGTCATGCCGC
GCCTGCGCATCAACTCGGTCTATGCCCAGGTCCTGGCCAGCAACCGCAGCGGCGATCATTCCGCCTTGCAATCGCAGTTG
CAGCAGGCGCGCTGGATGGTGCGCAACGTCGAACAGCGCTTCGACACCATCCTGCGGGTGTCGCAGGCCATCGTCGACCG
CCAGTCCGAATTCTTCTCGCACGGCGCCGGCGCCATGCAGCCGCTGATCCTGAAGGACATCGCGGGCGAGCTCGGCTTGC
ACGAGTCCACGATCTCGCGCGCGACCACCCAGAAGTTCATGCTGACACCCTTCGGCACCATGGAGCTCAAGCGCTTCTTC
GGCGCGGGGGTGGCCACGGAAGCGGGCGGCTCGGCGTCCGCCACCGCCGTGCAGGCGCAGATCCGCCAGATGGTGGCGGA
AGAGTCCGCGGGCAAGCCGCTGTCGGACAACCAGATCATGCAGATGCTGGCCGATCGCGGCATCGTCATCGCACGCCGAA
CGGTCGCCAAATACCGTGAAGCGCTGCGCATCGCCCCGGCATCCGTGCGCAAGGCACAGGCCTCGCTGCGCACCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(315-471)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(105-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3K1W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.295

100

0.536

Multiple sequence alignment