Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LF130101_RS01560 Genome accession   NZ_LT906621
Coordinates   327928..329301 (+) Length   457 a.a.
NCBI ID   WP_057194461.1    Uniprot ID   A0AAJ6A204
Organism   Limosilactobacillus fermentum strain IMDO 130101     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 322928..334301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF130101_RS01530 (LF130101_352) - 323524..324138 (-) 615 WP_057194463.1 sugar O-acetyltransferase -
  LF130101_RS01535 (LF130101_353) rpiA 324166..324852 (-) 687 WP_104877838.1 ribose-5-phosphate isomerase RpiA -
  LF130101_RS01540 (LF130101_354) tnpA 324887..325336 (-) 450 Protein_282 IS200/IS605 family transposase -
  LF130101_RS01545 (LF130101_355) - 325411..326661 (+) 1251 WP_046025890.1 RNA-guided endonuclease InsQ/TnpB family protein -
  LF130101_RS01550 (LF130101_356) - 326864..327175 (-) 312 WP_049182693.1 GNAT family N-acetyltransferase -
  LF130101_RS01555 (LF130101_357) - 327376..327915 (+) 540 WP_104878738.1 dUTP diphosphatase -
  LF130101_RS01560 (LF130101_358) radA 327928..329301 (+) 1374 WP_057194461.1 DNA repair protein RadA Machinery gene
  LF130101_RS01565 (LF130101_359) - 329333..330466 (+) 1134 WP_104877839.1 PIN/TRAM domain-containing protein -
  LF130101_RS01570 (LF130101_360) gltX 330537..332033 (+) 1497 WP_104877840.1 glutamate--tRNA ligase -
  LF130101_RS01575 - 332101..332670 (+) 570 Protein_289 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49415.82 Da        Isoelectric Point: 8.7358

>NTDB_id=1148187 LF130101_RS01560 WP_057194461.1 327928..329301(+) (radA) [Limosilactobacillus fermentum strain IMDO 130101]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTMLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKISLG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1148187 LF130101_RS01560 WP_057194461.1 327928..329301(+) (radA) [Limosilactobacillus fermentum strain IMDO 130101]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTATAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGGAAGTCCACCAT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTACGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGT
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGTCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACGGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTTCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.991

99.125

0.674
  radA Streptococcus mitis NCTC 12261

67.77

99.125

0.672
  radA Streptococcus pneumoniae Rx1

67.77

99.125

0.672
  radA Streptococcus pneumoniae D39

67.77

99.125

0.672
  radA Streptococcus pneumoniae R6

67.77

99.125

0.672
  radA Streptococcus pneumoniae TIGR4

67.77

99.125

0.672
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637

Multiple sequence alignment