Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CKV72_RS11305 Genome accession   NZ_LT906477
Coordinates   2296671..2298038 (-) Length   455 a.a.
NCBI ID   WP_089866468.1    Uniprot ID   -
Organism   Clostridium cochlearium strain NCTC13027     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2291671..2303038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV72_RS12625 ispD 2293035..2293693 (-) 659 Protein_2219 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CKV72_RS11290 (SAMEA4530647_02329) - 2293767..2294870 (-) 1104 WP_089866458.1 PIN/TRAM domain-containing protein -
  CKV72_RS11295 (SAMEA4530647_02330) - 2295143..2295541 (+) 399 WP_089866462.1 DUF1573 domain-containing protein -
  CKV72_RS11300 (SAMEA4530647_02331) disA 2295582..2296655 (-) 1074 WP_089866465.1 DNA integrity scanning diadenylate cyclase DisA -
  CKV72_RS11305 (SAMEA4530647_02332) radA 2296671..2298038 (-) 1368 WP_089866468.1 DNA repair protein RadA Machinery gene
  CKV72_RS11310 (SAMEA4530647_02333) nagB 2298172..2298897 (-) 726 WP_089866470.1 glucosamine-6-phosphate deaminase -
  CKV72_RS11315 (SAMEA4530647_02334) - 2299055..2301487 (-) 2433 WP_095178302.1 ATP-dependent Clp protease ATP-binding subunit -
  CKV72_RS11320 (SAMEA4530647_02335) - 2301516..2302538 (-) 1023 WP_089866476.1 protein arginine kinase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50512.53 Da        Isoelectric Point: 8.0583

>NTDB_id=1148001 CKV72_RS11305 WP_089866468.1 2296671..2298038(-) (radA) [Clostridium cochlearium strain NCTC13027]
MAKVKSCFVCQECGYESPKWLGKCPDCNTWNSMVEEIKSSKQADKKFSYNLNSNASESIVDIKSITYERISTGINELNRV
LGGGIVRGSLTLISGDPGIGKSTLLLQAANNIAKNYGKILYISGEESQEQIRLRGERLQALCKEVYILSETDINVILGNI
DKLKPKFIILDSIQTLYNPEISSAPGSVSQVRECANTIMRLAKTNNIPFFIVAHVTKQGALAGPKVLEHMVDTVLSFEGE
RTEEFRILRTIKNRFGNTSEIGVFEMRENGLSEITNPSEVFLEDISLNKEGSTIIGIMEGSRPILVEVQALVTKTNAPMP
RRTVVGVETSRINLILAVLEKKLKIPFYQFDVYINVVGGLNIEGTFADLGIALSLISSVKGIETKLDKMLVVGEIGLTGE
VRPVMFCDRIINEGKKMGFKNFIIPSRNKDKIIQDDRSINIIGTQYLRETINRVF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1148001 CKV72_RS11305 WP_089866468.1 2296671..2298038(-) (radA) [Clostridium cochlearium strain NCTC13027]
ATGGCTAAAGTAAAAAGTTGTTTTGTATGTCAAGAATGTGGATATGAATCCCCCAAGTGGTTAGGTAAGTGTCCAGATTG
TAATACTTGGAATAGTATGGTTGAGGAGATAAAAAGTAGTAAACAAGCAGATAAAAAGTTTAGTTATAACTTGAATAGTA
ATGCTTCGGAAAGCATAGTAGATATAAAGTCAATTACATATGAAAGAATCAGTACGGGAATTAATGAGTTAAATAGAGTA
CTAGGAGGAGGAATAGTTAGAGGTTCCTTAACTCTTATATCAGGTGATCCGGGTATAGGAAAATCGACTTTATTATTACA
AGCTGCCAATAATATAGCTAAAAATTATGGCAAGATATTATACATATCAGGAGAGGAATCTCAAGAGCAAATAAGACTAA
GAGGAGAAAGATTACAGGCTTTATGTAAAGAAGTATATATATTGTCTGAAACAGATATTAATGTTATATTAGGCAATATT
GATAAATTAAAACCTAAATTTATAATATTAGATTCAATTCAAACTTTATACAATCCAGAAATATCTTCAGCACCAGGAAG
TGTTTCACAAGTTAGAGAATGTGCAAATACAATTATGAGGTTAGCTAAAACTAATAACATACCGTTTTTTATAGTAGCTC
ATGTCACGAAACAAGGGGCATTAGCAGGCCCTAAAGTTTTAGAACATATGGTAGATACAGTTCTATCTTTCGAAGGAGAA
AGAACAGAAGAATTTAGAATACTTAGAACAATTAAGAATAGATTTGGGAACACTAGTGAAATTGGTGTGTTTGAAATGAG
GGAAAATGGACTTAGTGAAATAACTAATCCATCAGAAGTTTTTTTGGAAGATATAAGTCTTAATAAAGAAGGTTCAACTA
TAATAGGAATAATGGAAGGAAGTAGACCTATATTAGTAGAGGTTCAAGCATTAGTAACAAAAACAAATGCACCTATGCCA
AGAAGAACAGTAGTAGGAGTAGAAACATCTAGGATAAATTTAATTTTGGCAGTATTAGAAAAGAAATTAAAAATACCATT
TTATCAATTCGATGTGTATATAAATGTTGTTGGTGGGCTCAATATAGAAGGAACTTTTGCAGATTTAGGTATAGCATTAT
CATTAATATCTAGTGTAAAGGGAATAGAGACTAAATTAGATAAGATGTTGGTTGTAGGCGAAATAGGATTAACTGGAGAA
GTGAGACCAGTAATGTTTTGTGATAGAATAATAAATGAAGGCAAAAAGATGGGATTTAAAAATTTTATTATTCCTTCAAG
AAATAAGGATAAAATCATACAGGATGATAGAAGCATAAATATTATAGGAACACAATATTTAAGGGAAACAATAAATAGAG
TATTTTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-428)

AAA domain (AAA_25)

(61-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.559

99.78

0.495
  radA Streptococcus mitis NCTC 12261

48.571

100

0.486
  radA Streptococcus pneumoniae Rx1

48.571

100

0.486
  radA Streptococcus pneumoniae D39

48.571

100

0.486
  radA Streptococcus pneumoniae R6

48.571

100

0.486
  radA Streptococcus pneumoniae TIGR4

48.571

100

0.486
  radA Streptococcus mitis SK321

48.571

100

0.486

Multiple sequence alignment