Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CKV93_RS00880 Genome accession   NZ_LT906464
Coordinates   205822..207186 (+) Length   454 a.a.
NCBI ID   WP_095115196.1    Uniprot ID   A0A240BTX3
Organism   Staphylococcus muscae strain NCTC13833     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 200822..212186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV93_RS00860 (SAMEA4412661_00178) - 201193..201654 (+) 462 WP_095115188.1 CtsR family transcriptional regulator -
  CKV93_RS00865 (SAMEA4412661_00179) - 201675..202211 (+) 537 WP_095115190.1 UvrB/UvrC motif-containing protein -
  CKV93_RS00870 (SAMEA4412661_00180) - 202201..203205 (+) 1005 WP_095115192.1 protein arginine kinase -
  CKV93_RS00875 (SAMEA4412661_00181) - 203221..205668 (+) 2448 WP_095115194.1 ATP-dependent Clp protease ATP-binding subunit -
  CKV93_RS00880 (SAMEA4412661_00182) radA 205822..207186 (+) 1365 WP_095115196.1 DNA repair protein RadA Machinery gene
  CKV93_RS00885 (SAMEA4412661_00183) ispD 207238..207936 (+) 699 WP_095115198.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CKV93_RS00890 (SAMEA4412661_00184) ispF 207929..208411 (+) 483 WP_095115200.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  CKV93_RS00895 (SAMEA4412661_00185) gltX 208659..210113 (+) 1455 WP_095115203.1 glutamate--tRNA ligase -
  CKV93_RS00900 (SAMEA4412661_00187) cysE 210412..211053 (+) 642 WP_095115205.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49932.53 Da        Isoelectric Point: 7.2495

>NTDB_id=1147732 CKV93_RS00880 WP_095115196.1 205822..207186(+) (radA) [Staphylococcus muscae strain NCTC13833]
MAKKKVTFECTACGYQTPKWMGKCPNCGAWNSMEETIEQKPTSAKHGVRSESTAKVQKLNDVKQEQAPRILTKSRELNRV
LGGGIVQGSLVLIGGDPGIGKSTLLLQMCAELSKERKILYITGEESLNQTKLRADRLEEDASALNVFAETNLEIIHEAVK
KIEPELIVVDSIQTIFHPDITSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLHSVVNPSEMFLEERSSNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLTEPAVDLGIIIATATSFKDRAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPQTNIGGWTFPEGIEVIGVTSVHEALKYALKS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1147732 CKV93_RS00880 WP_095115196.1 205822..207186(+) (radA) [Staphylococcus muscae strain NCTC13833]
ATGGCAAAAAAGAAAGTGACTTTTGAGTGCACGGCGTGTGGCTATCAAACGCCGAAATGGATGGGGAAATGTCCTAACTG
CGGTGCATGGAACAGTATGGAAGAGACGATTGAGCAGAAGCCAACGAGTGCGAAACATGGTGTGCGCTCAGAATCGACAG
CCAAAGTGCAAAAACTGAATGATGTCAAACAAGAGCAAGCGCCACGTATTTTGACGAAAAGTCGGGAACTGAATCGTGTG
TTAGGTGGCGGTATTGTACAAGGTTCACTCGTATTGATTGGTGGAGATCCGGGTATTGGGAAGTCCACGTTATTACTGCA
AATGTGTGCAGAGTTATCTAAAGAACGTAAGATTTTGTATATTACAGGGGAAGAATCTTTGAATCAAACGAAGCTGCGTG
CCGATCGTCTAGAAGAAGATGCAAGTGCTTTGAACGTCTTTGCAGAGACGAATTTGGAAATCATTCATGAAGCAGTGAAA
AAGATAGAACCAGAGTTGATTGTCGTTGACTCGATTCAGACAATTTTCCATCCTGACATTACGTCGGCACCAGGATCTGT
GTCGCAAGTGCGAGAAAGTACGCAAAGTTTGATGCATATTGCGAAACAGATGAATATTGCGACGTTTATTGTTGGGCATG
TGACGAAAGAAGGTCAGATTGCAGGTCCACGTTTATTAGAGCATATGGTAGATACAGTTCTTTATTTTGAAGGAGATGAG
CATCATGCGTATCGTATTTTACGTGCGGTGAAAAACCGTTTCGGCTCTACGAATGAAATGGGCATTTTTGAAATGAAGCA
GTCTGGATTACACAGTGTCGTCAATCCATCAGAGATGTTTTTAGAAGAGCGCTCGTCCAACGTTCCAGGTTCGACAATTG
TTGCTACGATGGAAGGGACACGTCCATTGCTTATCGAAGTACAAGCGCTTGTGACACCGACGACTTTTAATAACCCACGT
CGGATGGCGACAGGTATTGATCACAATCGTTTAAGTTTGCTAATGGCGGTACTCGAGAAGAAAGAAGGATACTTGTTGCA
ACAACAAGATGCGTATATTAAAGTTGCTGGCGGTGTGAAGTTGACAGAGCCTGCCGTCGACTTAGGGATTATTATCGCCA
CAGCAACAAGTTTTAAAGATCGTGCGGTCGATGGCTTGGATTGTTTTGTAGGTGAAGTGGGCCTTACAGGGGAAGTGCGA
CGTGTGTCACGTATTGAGCAGCGTGTGCAAGAAGCTGCAAAGTTAGGTTTTAAGCGTGTCATTATTCCACAGACAAATAT
CGGAGGATGGACATTCCCAGAAGGTATAGAAGTTATTGGTGTGACATCAGTGCATGAAGCATTGAAATATGCGCTAAAAT
CATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(344-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240BTX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

100

0.661
  radA Streptococcus pneumoniae Rx1

59.556

99.119

0.59
  radA Streptococcus pneumoniae D39

59.556

99.119

0.59
  radA Streptococcus pneumoniae R6

59.556

99.119

0.59
  radA Streptococcus pneumoniae TIGR4

59.556

99.119

0.59
  radA Streptococcus mitis NCTC 12261

59.556

99.119

0.59
  radA Streptococcus mitis SK321

59.556

99.119

0.59

Multiple sequence alignment