Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CKV88_RS02315 Genome accession   NZ_LT906460
Coordinates   532242..533606 (+) Length   454 a.a.
NCBI ID   WP_002462524.1    Uniprot ID   -
Organism   Staphylococcus simiae strain NCTC13838     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 527242..538606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV88_RS02295 (SAMEA4384339_00465) - 527319..527780 (+) 462 WP_002462528.1 CtsR family transcriptional regulator -
  CKV88_RS02300 (SAMEA4384339_00466) - 527805..528356 (+) 552 WP_002462527.1 UvrB/UvrC motif-containing protein -
  CKV88_RS02305 (SAMEA4384339_00467) - 528361..529368 (+) 1008 WP_002462526.1 protein arginine kinase -
  CKV88_RS02310 (SAMEA4384339_00468) - 529382..531832 (+) 2451 WP_002462525.1 ATP-dependent Clp protease ATP-binding subunit -
  CKV88_RS02315 (SAMEA4384339_00469) radA 532242..533606 (+) 1365 WP_002462524.1 DNA repair protein RadA Machinery gene
  CKV88_RS02320 (SAMEA4384339_00470) - 533631..534689 (+) 1059 WP_002462523.1 PIN/TRAM domain-containing protein -
  CKV88_RS02325 (SAMEA4384339_00471) gltX 535083..536537 (+) 1455 WP_002462522.1 glutamate--tRNA ligase -
  CKV88_RS02330 (SAMEA4384339_00473) cysE 537567..538229 (+) 663 WP_197697105.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49815.24 Da        Isoelectric Point: 6.8755

>NTDB_id=1147625 CKV88_RS02315 WP_002462524.1 532242..533606(+) (radA) [Staphylococcus simiae strain NCTC13838]
MAKKKVIFECMACGYQSPKWMGKCPNCGGWNQMEEIVEKASSPKHGVKTKEVASKVQKLNSIHHESTPRILTESAEFNRV
LGGGIVNGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLNQTKLRADRLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSANVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWSFPEGIQVIGVTSVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1147625 CKV88_RS02315 WP_002462524.1 532242..533606(+) (radA) [Staphylococcus simiae strain NCTC13838]
TTGGCCAAGAAAAAAGTGATTTTCGAATGTATGGCTTGCGGATACCAATCACCAAAATGGATGGGTAAATGTCCAAACTG
TGGTGGTTGGAATCAAATGGAAGAAATTGTTGAAAAAGCATCTAGTCCAAAACACGGTGTGAAAACAAAAGAAGTTGCAA
GTAAAGTACAAAAGTTAAATAGCATTCATCATGAATCAACACCTAGAATATTAACTGAATCAGCAGAATTCAATCGCGTG
CTAGGTGGAGGAATTGTTAATGGTTCTCTAGTTTTAATCGGAGGAGATCCTGGTATTGGGAAATCAACCCTATTACTTCA
AATTTGTGCATCATTATCACAAAAGAAAAAAGTGTTATATATAACAGGTGAAGAATCATTAAATCAAACTAAATTACGGG
CTGATCGTTTAGATGAAGATTCAAGTGAATTGCAAGTTTTAGCTGAAACAGACTTAGAAGTTATTTATCAAACAGTGAAA
GAAGAACAACCAGATTTATTAGTTGTAGACTCTATTCAAACTATTTATCATCCGGAAATATCATCAGCGCCTGGTTCAGT
TTCACAAGTGAGAGAGAGTACACAAAGTTTAATGAATATTGCAAAACAAATGAATATTGCAACATTCATTGTTGGTCATG
TTACGAAAGAAGGACAAATTGCTGGTCCGCGTTTATTAGAACATATGGTTGATACTGTTTTATATTTTGAAGGTGATGAA
CATCATGCGTATAGAATTCTACGAGCAGTAAAAAATCGTTTTGGTTCAACGAATGAAATGGGTATTTTTGAAATGAAACA
AAGTGGTTTGAAAGGTGTCAATAACCCATCTGAGATGTTTTTAGAAGAACGTTCAGCTAATGTACCAGGATCTACAATAG
TAGCAACAATGGAAGGTACAAGACCATTACTTATTGAAGTTCAAGCTTTAGTTACACCAACGACTTTTAATAATCCGAGA
AGAATGGCAACAGGAATAGATCATAATAGGCTTAGCTTATTAATGGCAGTACTTGAAAAAAAGGAAAACTACTTATTACA
ACAACAAGACGCTTATATTAAAGTTGCCGGTGGAGTTAAATTGACAGAACCTGCTGTTGACTTAAGTGTAATTGTTGCGA
CAGCATCTAGTTTTAAAGATAAAGCTGTAGATGGTTTAGACTGTTATATAGGAGAAGTTGGTTTGACTGGAGAAGTTAGA
AGAGTTTCACGTATAGAACAACGTGTACAAGAAGCAGCGAAGCTTGGATTTAAGAGAGTAATTATACCTAAAAATAATAT
CGGAGGCTGGTCATTTCCAGAAGGTATACAGGTTATTGGTGTAACATCGGTTCATGAAGCATTATCATTTGCCCTACATT
CATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(69-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.009

100

0.663
  radA Streptococcus pneumoniae Rx1

61.607

98.678

0.608
  radA Streptococcus pneumoniae D39

61.607

98.678

0.608
  radA Streptococcus pneumoniae R6

61.607

98.678

0.608
  radA Streptococcus pneumoniae TIGR4

61.607

98.678

0.608
  radA Streptococcus mitis NCTC 12261

61.607

98.678

0.608
  radA Streptococcus mitis SK321

61.607

98.678

0.608

Multiple sequence alignment