Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CKV65_RS04570 Genome accession   NZ_LT906446
Coordinates   972996..974420 (+) Length   474 a.a.
NCBI ID   WP_051177653.1    Uniprot ID   A0A239TKX2
Organism   Megamonas hypermegale strain NCTC10570     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 967996..979420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV65_RS04550 (SAMEA4364220_00928) - 968256..968702 (+) 447 WP_027890776.1 CtsR family transcriptional regulator -
  CKV65_RS04555 (SAMEA4364220_00929) - 968721..969272 (+) 552 WP_027890777.1 UvrB/UvrC motif-containing protein -
  CKV65_RS04560 (SAMEA4364220_00930) - 969265..970341 (+) 1077 WP_027890778.1 ATP--guanido phosphotransferase -
  CKV65_RS04565 (SAMEA4364220_00931) - 970359..972860 (+) 2502 WP_027890779.1 ATP-dependent Clp protease ATP-binding subunit -
  CKV65_RS10810 (SAMEA4364220_00932) - 972838..972984 (+) 147 WP_169449333.1 hypothetical protein -
  CKV65_RS04570 (SAMEA4364220_00933) radA 972996..974420 (+) 1425 WP_051177653.1 DNA repair protein RadA Machinery gene
  CKV65_RS04575 (SAMEA4364220_00934) - 974389..974862 (+) 474 WP_027890780.1 GNAT family N-acetyltransferase -
  CKV65_RS04580 (SAMEA4364220_00935) - 974965..976101 (+) 1137 WP_027890781.1 PIN/TRAM domain-containing protein -
  CKV65_RS04585 (SAMEA4364220_00936) ispD 976161..976907 (+) 747 WP_027890782.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CKV65_RS04590 (SAMEA4364220_00937) ispF 976996..977475 (+) 480 WP_027890783.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  CKV65_RS04595 (SAMEA4364220_00938) gltX 977557..979038 (+) 1482 WP_027890784.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52706.68 Da        Isoelectric Point: 7.8313

>NTDB_id=1147315 CKV65_RS04570 WP_051177653.1 972996..974420(+) (radA) [Megamonas hypermegale strain NCTC10570]
MAKRKTLYVCQECGYSVPKWLGKCPGCGKWNTLVEELEEPSSFTNINRNFSLNTNKTNSIPKPIKTIETEDLPRFSAGSP
EMNRVLGGGIIPGSLVLMVGDPGVGKSSLNLRICAYVAKKHKVLYVTGEESSRQIRMRADRLNALDDNLYVLSETDLERI
ERHVLEQKPDLLVIDSIQTIFRPDIQSAPGSVSQVRECSVNLLRIAKQNNIAIFIIGHVTKDGTLAGPRVLEHIVDTVLY
FEGERNAEYRVLRAVKNRFGNTNEIGIFEMRENGLVDVPDASKMFLSEQNDNTSGTVIIPALEGTRPLLVEIQALVAPTP
YVPPRRTSDSIDIKKIQLLLAVLEKRVHLNIGSCDVYVKVAGGIKIDDPAVDLALCCAMASSFRNQNIKPQTIIFGEVGL
AGEVRSVSQPDIRVREAIRMGFTNIILPAKNLDRLEHSSSNVQIHGVRYLSEALKIAFNLEVFYNEYDNSPHKK

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=1147315 CKV65_RS04570 WP_051177653.1 972996..974420(+) (radA) [Megamonas hypermegale strain NCTC10570]
ATGGCTAAAAGAAAAACTCTTTACGTTTGTCAAGAATGTGGTTATAGTGTGCCAAAATGGCTGGGCAAATGTCCCGGCTG
TGGCAAATGGAATACACTTGTAGAGGAACTGGAAGAACCCAGTTCCTTTACTAATATAAACCGCAATTTCAGTTTAAATA
CAAATAAGACAAACTCTATCCCAAAACCGATAAAAACAATTGAAACAGAAGATTTACCTAGATTTTCCGCAGGCTCACCT
GAAATGAACCGCGTATTAGGTGGTGGCATAATACCTGGCTCATTAGTGCTGATGGTAGGTGACCCTGGCGTTGGTAAATC
AAGTTTGAATTTACGCATATGTGCATATGTAGCTAAAAAACACAAAGTTTTATACGTAACGGGCGAAGAAAGTTCCCGCC
AAATTCGCATGCGCGCTGACCGCTTAAACGCATTAGATGACAATTTATATGTACTAAGTGAAACGGATTTAGAGCGCATT
GAACGCCATGTATTAGAGCAAAAACCTGATTTACTCGTCATCGATTCCATTCAGACTATTTTTCGCCCCGACATTCAAAG
TGCGCCTGGCAGTGTAAGCCAAGTCCGCGAATGTTCCGTCAATCTTTTGCGCATTGCCAAGCAAAATAATATAGCCATTT
TTATAATTGGTCATGTTACAAAAGACGGTACACTCGCTGGTCCGCGTGTACTAGAACATATCGTCGATACAGTATTGTAT
TTCGAAGGTGAACGCAATGCTGAATACCGCGTTTTGCGCGCTGTAAAAAATCGTTTTGGCAATACAAATGAAATCGGCAT
TTTTGAAATGCGCGAAAATGGATTAGTTGATGTTCCCGATGCTTCAAAAATGTTTTTATCCGAACAAAATGATAACACTT
CGGGAACTGTCATAATTCCTGCATTAGAAGGAACGCGTCCACTACTTGTAGAAATTCAAGCTCTTGTGGCACCAACGCCT
TATGTTCCACCGCGCAGAACGTCTGACTCTATCGATATAAAAAAAATACAATTATTATTAGCTGTATTAGAAAAACGTGT
TCATTTAAATATCGGCAGTTGTGATGTCTACGTTAAAGTGGCTGGTGGCATAAAAATAGATGACCCTGCTGTCGACCTCG
CGCTATGCTGTGCCATGGCATCGAGTTTTCGCAACCAAAATATCAAACCACAAACCATTATTTTCGGTGAAGTGGGACTG
GCTGGTGAAGTACGTTCTGTAAGCCAACCAGATATCAGAGTACGCGAAGCCATTCGCATGGGATTTACAAATATCATCTT
GCCTGCTAAAAATTTAGACCGCCTAGAACATTCCTCTTCCAATGTACAAATTCATGGCGTTCGCTATTTAAGTGAAGCTT
TAAAAATCGCATTTAATTTAGAGGTGTTTTATAATGAATACGATAATTCACCTCATAAAAAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(355-457)

AAA domain (AAA_25)

(75-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239TKX2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.609

97.046

0.511
  radA Streptococcus pneumoniae Rx1

49.348

97.046

0.479
  radA Streptococcus pneumoniae D39

49.348

97.046

0.479
  radA Streptococcus pneumoniae R6

49.348

97.046

0.479
  radA Streptococcus pneumoniae TIGR4

49.348

97.046

0.479
  radA Streptococcus mitis NCTC 12261

49.348

97.046

0.479
  radA Streptococcus mitis SK321

49.348

97.046

0.479

Multiple sequence alignment