Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CKW02_RS08805 Genome accession   NZ_LT906438
Coordinates   1752998..1753378 (+) Length   126 a.a.
NCBI ID   WP_223251255.1    Uniprot ID   -
Organism   Bacillus pumilus strain NCTC10337     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1747998..1758378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW02_RS08795 (SAMEA4076707_01782) - 1748488..1751631 (+) 3144 WP_003211586.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  CKW02_RS08800 (SAMEA4076707_01783) - 1751828..1752736 (+) 909 WP_003212025.1 trypsin-like serine peptidase -
  CKW02_RS08805 (SAMEA4076707_01784) nucA/comI 1752998..1753378 (+) 381 WP_223251255.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CKW02_RS08810 (SAMEA4076707_01785) - 1753527..1754375 (+) 849 WP_003211492.1 STAS domain-containing protein -
  CKW02_RS08815 (SAMEA4076707_01786) - 1754409..1754951 (-) 543 WP_003211360.1 IseA DL-endopeptidase inhibitor family protein -
  CKW02_RS08820 - 1755246..1755680 (+) 435 WP_003212431.1 hypothetical protein -
  CKW02_RS08825 (SAMEA4076707_01787) lexA 1755726..1756346 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  CKW02_RS08830 (SAMEA4076707_01788) yneA 1756506..1756817 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  CKW02_RS08835 (SAMEA4076707_01789) - 1756835..1757482 (+) 648 WP_003211322.1 recombinase family protein -
  CKW02_RS08840 (SAMEA4076707_01790) - 1757552..1757782 (+) 231 WP_003211509.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13751.39 Da        Isoelectric Point: 7.3841

>NTDB_id=1147082 CKW02_RS08805 WP_223251255.1 1752998..1753378(+) (nucA/comI) [Bacillus pumilus strain NCTC10337]
MGTLLGGFGEEHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=1147082 CKW02_RS08805 WP_223251255.1 1752998..1753378(+) (nucA/comI) [Bacillus pumilus strain NCTC10337]
ATGGGCACGCTATTAGGTGGATTTGGGGAGGAACATGCGGCAAAAGGTGCCGATCGATATGATCATGTCATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGCATTCCGACAAAACCTGGCTTTGACCGGGATGAGTGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGATTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.143

94.444

0.54

Multiple sequence alignment