Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CKW10_RS02750 Genome accession   NZ_LT906435
Coordinates   596994..598484 (-) Length   496 a.a.
NCBI ID   WP_039395518.1    Uniprot ID   A0A239S8W9
Organism   Pandoraea sputorum strain NCTC13161     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 591994..603484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW10_RS02720 (SAMEA4530655_00544) mutY 591996..593153 (+) 1158 WP_039395500.1 A/G-specific adenine glycosylase -
  CKW10_RS02725 (SAMEA4530655_00545) rapZ 593170..594042 (-) 873 WP_039395504.1 RNase adapter RapZ -
  CKW10_RS02730 (SAMEA4530655_00546) - 594234..594566 (-) 333 WP_039395507.1 PsiF family protein -
  CKW10_RS02735 (SAMEA4530655_00547) hprK 594749..595714 (-) 966 WP_039395509.1 HPr(Ser) kinase/phosphatase -
  CKW10_RS02740 (SAMEA4530655_00548) ptsN 595805..596260 (-) 456 WP_039395512.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CKW10_RS02745 (SAMEA4530655_00549) hpf 596496..596852 (-) 357 WP_039395515.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CKW10_RS02750 (SAMEA4530655_00550) rpoN/rpoN1 596994..598484 (-) 1491 WP_039395518.1 RNA polymerase factor sigma-54 Machinery gene
  CKW10_RS02755 (SAMEA4530655_00551) lptB 598578..599348 (-) 771 WP_039402130.1 LPS export ABC transporter ATP-binding protein -
  CKW10_RS02760 (SAMEA4530655_00552) lptA 599383..600075 (-) 693 WP_052252464.1 lipopolysaccharide transport periplasmic protein LptA -
  CKW10_RS02765 (SAMEA4530655_00553) lptC 600072..600749 (-) 678 WP_039395522.1 LPS export ABC transporter periplasmic protein LptC -
  CKW10_RS02770 (SAMEA4530655_00554) - 600774..601259 (-) 486 WP_052253166.1 HAD family hydrolase -
  CKW10_RS02775 (SAMEA4530655_00555) - 601381..602361 (-) 981 WP_039395525.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55111.96 Da        Isoelectric Point: 5.1828

>NTDB_id=1146954 CKW10_RS02750 WP_039395518.1 596994..598484(-) (rpoN/rpoN1) [Pandoraea sputorum strain NCTC13161]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERSSSSGSSNDN
GPEASNGHDEARELDGEARFDDNASDNGSDWSLNDFARSGSASDDDDNARPQLHVDHPNLREHLLAQLRLTKASPRDRAL
VEFLIESLDEDGYLGSLLDEIQAEMPEELALETEEINAALALLQSFDPAGVGARSPAECLRLQLQRLPASGVRTLSLRIV
TDHLELLAARDYTRLRRLLGVGEDGLRAAHQLIRSLDPFPGAAYGAPQADYVVPDVLVRKQGSGWTAELNPEVMPRLRIN
EMYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHEST
ISRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAK
YREAMRIPAVNLRKSL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=1146954 CKW10_RS02750 WP_039395518.1 596994..598484(-) (rpoN/rpoN1) [Pandoraea sputorum strain NCTC13161]
ATGAAACCGACTCTTCAACTCCGCACCTCTCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCGATCCGGTTGTTGCA
ACTGTCGACGCTCGAACTTCAACAGGAAGTCGAGCACGCCCTTACCCAGAACCCGATGCTCGAACGCGAGGACGATTGGC
TCGCCGGTTCGGTACGTGTGGGCACAGACGGGTCGCTGCGCAACGAACGGAGTTCGTCGTCGGGCAGTTCGAACGACAAT
GGCCCCGAGGCCTCCAACGGTCACGACGAAGCACGCGAACTCGATGGTGAGGCCCGCTTCGACGACAACGCGTCGGACAA
CGGCAGCGACTGGAGCCTGAACGACTTCGCCCGCTCCGGCAGCGCATCGGACGACGACGACAATGCGCGTCCGCAACTTC
ACGTCGACCACCCCAATCTCCGCGAACATCTGCTGGCCCAACTGCGCCTGACCAAGGCGAGTCCGCGCGACCGCGCGCTG
GTGGAATTCCTGATCGAATCGCTGGACGAAGACGGCTACCTCGGTAGCCTGCTCGACGAAATTCAGGCCGAAATGCCTGA
AGAACTGGCGCTCGAAACGGAAGAGATCAACGCGGCGCTGGCGCTGCTGCAAAGCTTCGACCCGGCGGGCGTGGGTGCCC
GCAGCCCGGCCGAATGCCTGCGTCTACAGCTCCAGCGTCTGCCCGCTAGCGGTGTGCGCACCCTGTCGCTGCGCATCGTC
ACCGATCACCTGGAACTGCTGGCCGCGCGCGATTACACGCGTCTGCGCCGCCTGCTAGGTGTCGGTGAAGACGGCCTGCG
CGCCGCGCATCAGCTGATTCGCTCGCTGGACCCCTTCCCGGGCGCGGCTTACGGCGCGCCTCAGGCCGACTATGTCGTGC
CGGACGTGCTGGTACGCAAGCAAGGGAGCGGCTGGACGGCGGAGCTGAATCCGGAAGTCATGCCGCGTCTGCGTATCAAC
GAGATGTATGCGCGCATTCTCCGAAATAACCGGAACGATCCGGGAACCGGCAACCTCCAACAGCAGTTGCAGGAAGCGCG
CTGGCTGATCAAGAACATCCAGCAACGCTTCGACACGATCCTGCGGGTGGCGCAGGCGATTGTCGAGCGCCAAAAGAACT
TCTTCACGCATGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACATGAATCCACG
ATTTCACGCGTGACAACGAGTAAGTACATGCTCACTCCATTTGGAACCTTCGAGCTCAAGTACTTCTTCGGATCACACGT
GGCAACGGAAGCCGGAGGCGCTGCGTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGC
AGAGCCCCCTTTCCGATAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGTTTTGTGGTGGCGCGGCGCACTGTCGCGAAG
TACCGCGAGGCCATGCGAATCCCCGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(134-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-494)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239S8W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

69.062

100

0.698

Multiple sequence alignment