Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DXE33_RS07115 Genome accession   NZ_LT615228
Coordinates   1377264..1378154 (+) Length   296 a.a.
NCBI ID   WP_114639276.1    Uniprot ID   -
Organism   Polynucleobacter necessarius isolate PPGSP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1372264..1383154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE33_RS07085 pth 1372478..1373068 (+) 591 WP_114639270.1 aminoacyl-tRNA hydrolase -
  DXE33_RS07090 - 1373078..1373344 (-) 267 WP_114639271.1 YfhL family 4Fe-4S dicluster ferredoxin -
  DXE33_RS07095 coaD 1373361..1373852 (-) 492 WP_114639272.1 pantetheine-phosphate adenylyltransferase -
  DXE33_RS07100 rsmD 1373889..1374527 (-) 639 WP_114639273.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  DXE33_RS07105 - 1374499..1375836 (-) 1338 WP_114639274.1 pitrilysin family protein -
  DXE33_RS07110 - 1375833..1377200 (-) 1368 WP_114639275.1 pitrilysin family protein -
  DXE33_RS07115 pilA 1377264..1378154 (+) 891 WP_114639276.1 signal recognition particle-docking protein FtsY Machinery gene
  DXE33_RS07120 rpoH 1378248..1379171 (+) 924 WP_114639277.1 RNA polymerase sigma factor RpoH -
  DXE33_RS07125 - 1379182..1379772 (-) 591 WP_114639278.1 SCO family protein -
  DXE33_RS07130 cyoE 1379774..1380671 (-) 898 Protein_1465 heme o synthase -
  DXE33_RS07135 - 1380668..1381798 (-) 1131 WP_114639279.1 heme A synthase -
  DXE33_RS07140 - 1381798..1382454 (-) 657 WP_114639280.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31463.09 Da        Isoelectric Point: 7.1200

>NTDB_id=1145341 DXE33_RS07115 WP_114639276.1 1377264..1378154(+) (pilA) [Polynucleobacter necessarius isolate PPGSP1]
MFGLRKTLGSLFKSSKTDEAWFDSLEESLIQSDVGLPTTEQLITKLRKAAKSEKASSPEELQNLLIQEVASLLQTLEPTP
NPLFTSLKEAIPEVWLVVGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHAAVSRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHTALGLTG
IIVTKLDGTAKGGVICALAHTLKDGPKPAIFALGKGEGIEDLVPFTSGQYSAELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1145341 DXE33_RS07115 WP_114639276.1 1377264..1378154(+) (pilA) [Polynucleobacter necessarius isolate PPGSP1]
ATGTTTGGCCTACGTAAAACCCTCGGATCCCTATTCAAATCTAGTAAGACAGATGAAGCTTGGTTTGATAGCTTAGAAGA
ATCGCTGATTCAAAGCGATGTGGGCTTACCCACAACTGAACAATTGATTACGAAGTTGCGTAAAGCAGCCAAATCAGAAA
AAGCCTCAAGCCCCGAAGAATTACAAAATCTGCTGATTCAAGAAGTAGCCAGCCTACTTCAAACGCTTGAACCTACTCCA
AACCCCTTGTTTACCAGCCTTAAAGAGGCTATACCAGAGGTTTGGCTTGTGGTTGGAGTCAATGGTGCTGGCAAAACGAC
CACGATTGGCAAGCTTTGTAGGCTTTTTCAGTCGCAGGGAAAGTCAGTATTACTGGCTGCAGGAGATACCTTTAGAGCAG
CAGCACGTAATCAACTTCAAGAGTGGGGTGGGCGCAATCAAGTCGACGTTATTACCCAAGAAGGTGGGGATGCCGCAGCC
GTTGCCCATGATGCTATTCATGCTGCCGTATCTCGCAAAAGCGATATTTTGATCATTGATACTGCCGGTAGACTTGCTAC
CCAAGATCATTTGATGGAAGAGCTCAAGAAAGTAAAACGCGTTATTGGCAAGGCATTACCCGGGGCTCCACACCATACCT
TGCTGGTGCTAGATGGTAATACTGGCCAAAATGGTTTGAGCCAAGTCAAAGCCTTTCATACAGCACTAGGGCTTACTGGA
ATCATCGTCACCAAGCTGGATGGGACTGCCAAAGGCGGCGTGATCTGCGCCCTCGCTCACACCCTCAAAGATGGTCCAAA
ACCAGCAATTTTTGCCCTAGGCAAAGGCGAAGGAATTGAGGATTTAGTCCCATTCACATCAGGGCAATATTCTGCAGAAT
TATTTAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(92-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.381

99.324

0.52

Multiple sequence alignment