Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DXE27_RS05865 Genome accession   NZ_LT615227
Coordinates   1111116..1112480 (+) Length   454 a.a.
NCBI ID   WP_128113275.1    Uniprot ID   -
Organism   Polynucleobacter necessarius isolate PPGSP8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1106116..1117480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE27_RS10055 - 1106723..1107298 (+) 576 WP_197712327.1 ComEC/Rec2 family competence protein -
  DXE27_RS09195 - 1107322..1107693 (+) 372 WP_197712329.1 ComEC/Rec2 family competence protein -
  DXE27_RS05845 - 1107926..1108294 (+) 369 WP_128113272.1 glycine zipper family protein -
  DXE27_RS05855 - 1108730..1110571 (+) 1842 WP_415064466.1 potassium transporter Kup -
  DXE27_RS05860 - 1110619..1111077 (+) 459 WP_231969684.1 hypothetical protein -
  DXE27_RS05865 radA 1111116..1112480 (+) 1365 WP_128113275.1 DNA repair protein RadA Machinery gene
  DXE27_RS05870 prmB 1112497..1113393 (-) 897 WP_128113276.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  DXE27_RS05875 dapE 1113439..1114590 (-) 1152 WP_128113277.1 succinyl-diaminopimelate desuccinylase -
  DXE27_RS05880 dapD 1114600..1115427 (-) 828 WP_128113278.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48210.00 Da        Isoelectric Point: 7.3470

>NTDB_id=1145326 DXE27_RS05865 WP_128113275.1 1111116..1112480(+) (radA) [Polynucleobacter necessarius isolate PPGSP8]
MAKVKAVYICQSCGGTSAKWQGQCPSCQAWNTMEEGLPETTSNSRFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLKVIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1145326 DXE27_RS05865 WP_128113275.1 1111116..1112480(+) (radA) [Polynucleobacter necessarius isolate PPGSP8]
TTGGCTAAAGTCAAAGCGGTTTATATCTGTCAATCTTGCGGCGGCACCTCTGCTAAGTGGCAAGGTCAGTGTCCGTCTTG
TCAGGCATGGAACACAATGGAGGAGGGTCTTCCAGAGACCACTTCAAATTCTCGCTTTCAAGGATTGGCGCAATCACTGC
CAAGGCAGAAGCTATCTGCCATTACGGCTGAAGACTTGCCAAGATTTAGTACTGGTGTAGAAGAGTTTGACCGCGTTCTC
GGTGGTGGTTTGGTTCCTGGCGGAGTGGTGCTTCTCGGCGGCGATCCTGGCATTGGCAAATCCACATTGCTGCTTCAAGC
GCTGGCAGAGATGAGCTCTGCTGGTATGAATGTGCTCTATAGCAGCGGTGAAGAATCTGCTGCACAGATTGCTTTGCGAG
CAAAGCGTATAGCCTTGGACGCGCCCCAGCTTGAAGTATTGGCGGAGATTCAGTTGGAGAAACTGATCTCTATTATGGAT
ACCGTCAAACCACAAGTCCTGGTAGTGGATTCGATCCAGACTTTATATTCAGAAGTATTGAGCTCTGCCCCTGGCTCTGT
AGCCCAGGTCCGCGAGTGTGCGGCGCAACTCACGCGAGCTGCTAAATCTAGTGGCATCTGTGTATTGATGGTGGGACACG
TGACTAAAGATGGCCATTTGGCTGGTCCAAGAGTATTGGAGCATATCGTCGACACCGTTCTCTATTTTGAAGGTGACACG
CATTCCTCTTTCCGCTTGGTGCGCTCTATTAAGAATCGCTTTGGCGCTGTTAATGAGTTAGGTGTATTTGCGATGACTGA
AAAAGGTCTGCGCGGCGTGACTAATCCGTCAGCAATCTTCTTGTCGCAACATGAGCAAATGGTTCCTGGTGCTTGCGTAT
TGGTAACGCAAGAAGGTAGCAGGCCTTTGTTGGTAGAGATTCAGGCTTTGGTGGATACAGCGCATGTCCCCAATCCACGT
CGTTTGGCTGTTGGCTTGGAGCAGGCTCGTCTAGCCATGTTGCTGGCAGTGCTGCATCGTCATGCTGGTGTTGCTTGTTT
TGATCAAGATGTCTTCTTAAATGCAGTTGGTGGCGTCAAGATTTCTGAGCCAGCTGCTGACTTGGCTGTCCTCTTGGCTA
TTCAGTCATCTATTCGTAATCGTGCGTTACCAAAAGAGTTGATCGTATTTGGTGAAGTAGGTTTGGCCGGGGAGATTCGC
CCTTGCCCACGGGGTCAGGAGCGCTTGAAAGAGGCTGCTAAATTAGGCTTTACTGTGGCGATTATTCCAAAAGCCAATAT
GCCCAAAACTAAGATCCCGGGATTAAAGGTTATCCCTGTTGAGCGGATTGATCAGGCGATTGCTGCCGCAGCAGAACTCA
GCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.558

99.559

0.493
  radA Streptococcus pneumoniae Rx1

46

99.119

0.456
  radA Streptococcus pneumoniae D39

46

99.119

0.456
  radA Streptococcus pneumoniae R6

46

99.119

0.456
  radA Streptococcus pneumoniae TIGR4

46

99.119

0.456
  radA Streptococcus mitis NCTC 12261

46

99.119

0.456
  radA Streptococcus mitis SK321

46

99.119

0.456

Multiple sequence alignment