Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DXE27_RS02640 Genome accession   NZ_LT615227
Coordinates   520072..520962 (+) Length   296 a.a.
NCBI ID   WP_128112798.1    Uniprot ID   -
Organism   Polynucleobacter necessarius isolate PPGSP8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 515072..525962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE27_RS02610 pth 515263..515856 (+) 594 WP_128112793.1 aminoacyl-tRNA hydrolase -
  DXE27_RS02615 - 515874..516140 (-) 267 WP_128112794.1 YfhL family 4Fe-4S dicluster ferredoxin -
  DXE27_RS02620 coaD 516157..516654 (-) 498 WP_128112795.1 pantetheine-phosphate adenylyltransferase -
  DXE27_RS02625 rsmD 516691..517332 (-) 642 WP_231969619.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  DXE27_RS02630 - 517304..518641 (-) 1338 WP_128112796.1 M16 family metallopeptidase -
  DXE27_RS02635 - 518641..520008 (-) 1368 WP_128112797.1 M16 family metallopeptidase -
  DXE27_RS02640 pilA 520072..520962 (+) 891 WP_128112798.1 signal recognition particle-docking protein FtsY Machinery gene
  DXE27_RS02645 rpoH 521060..521983 (+) 924 WP_128112799.1 RNA polymerase sigma factor RpoH -
  DXE27_RS02650 - 522002..522586 (-) 585 WP_128112800.1 SCO family protein -
  DXE27_RS02655 cyoE 522594..523487 (-) 894 WP_128112801.1 heme o synthase -
  DXE27_RS02660 - 523490..524611 (-) 1122 WP_128112802.1 COX15/CtaA family protein -
  DXE27_RS02665 - 524611..525267 (-) 657 WP_128112803.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31359.83 Da        Isoelectric Point: 6.8444

>NTDB_id=1145323 DXE27_RS02640 WP_128112798.1 520072..520962(+) (pilA) [Polynucleobacter necessarius isolate PPGSP8]
MFGLRKTLGSLFKSSKVDEAWFDHLEESLIQSDVGLPTTEQLINKLRKAAKSEKASSPEELQSLLIQEVSTLLTAIETSP
NPLFISQKANTPEVWLVVGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLQEWGDRNQVDVITQDGGDAAA
VAHDAIHAAVSRKNDILIIDTAGRLATQDNLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQDGPKPAVLALGKGEGIDDLAPFTAGQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1145323 DXE27_RS02640 WP_128112798.1 520072..520962(+) (pilA) [Polynucleobacter necessarius isolate PPGSP8]
ATGTTCGGCCTACGTAAAACCCTCGGATCTCTTTTCAAATCAAGCAAAGTTGACGAAGCTTGGTTTGATCATTTAGAAGA
ATCACTCATTCAAAGTGATGTGGGGCTACCTACGACCGAACAACTCATCAACAAGCTACGCAAAGCAGCGAAATCTGAAA
AAGCCTCAAGCCCTGAGGAGTTGCAATCACTGCTCATTCAAGAGGTAAGCACGCTCCTTACGGCTATTGAAACTTCACCA
AACCCACTATTTATAAGCCAGAAAGCCAATACACCAGAGGTATGGCTGGTTGTAGGGGTAAACGGTGCAGGCAAGACAAC
AACAATAGGCAAGCTTTGCAAACTCTTTCAGTCTCAAGGTAAATCTGTTCTCTTGGCCGCTGGCGATACCTTTAGAGCTG
CCGCCCGCAATCAACTACAAGAGTGGGGTGATCGCAATCAGGTAGACGTTATCACCCAGGATGGCGGTGATGCTGCCGCT
GTTGCGCATGATGCTATTCATGCTGCCGTTTCTCGCAAAAATGACATTCTTATTATTGATACCGCAGGCCGTCTTGCCAC
GCAGGACAACCTGATGGAAGAGCTGAAGAAGGTAAAGCGCGTGATCGGCAAGGCACTTCCTGGTGCGCCACACCACACTC
TGCTTGTTTTAGATGGCAACACAGGGCAAAACGGCTTAAGCCAGGTCAAAGCCTTTCATGCTGCCCTGGGCCTAACTGGG
ATCATCGTTACCAAGCTGGATGGGACTGCCAAGGGCGGTGTTATCTGCGCCCTAGCCCACACCCTACAAGATGGTCCAAA
ACCCGCTGTGCTAGCCCTAGGGAAGGGCGAAGGAATTGATGATTTAGCCCCCTTTACAGCAGGGCAATATTCATCCGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-295)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.218

98.986

0.517

Multiple sequence alignment