Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DXE44_RS08540 Genome accession   NZ_LT606946
Coordinates   1648313..1649203 (+) Length   296 a.a.
NCBI ID   WP_114654058.1    Uniprot ID   -
Organism   Polynucleobacter necessarius isolate PPGSP5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643313..1654203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE44_RS08510 pth 1643502..1644098 (+) 597 WP_114654053.1 aminoacyl-tRNA hydrolase -
  DXE44_RS08515 - 1644103..1644372 (-) 270 WP_114654054.1 YfhL family 4Fe-4S dicluster ferredoxin -
  DXE44_RS08520 coaD 1644390..1644884 (-) 495 WP_114654055.1 pantetheine-phosphate adenylyltransferase -
  DXE44_RS08525 rsmD 1644921..1645550 (-) 630 WP_114654056.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  DXE44_RS11090 - 1645543..1646202 (-) 660 WP_331851860.1 M16 family metallopeptidase -
  DXE44_RS11095 - 1646129..1646878 (-) 750 WP_269460682.1 M16 family metallopeptidase -
  DXE44_RS08535 - 1646865..1648250 (-) 1386 WP_114654057.1 M16 family metallopeptidase -
  DXE44_RS08540 pilA 1648313..1649203 (+) 891 WP_114654058.1 signal recognition particle-docking protein FtsY Machinery gene
  DXE44_RS08545 rpoH 1649291..1650232 (+) 942 WP_114654059.1 RNA polymerase sigma factor RpoH -
  DXE44_RS08550 - 1650255..1650818 (-) 564 WP_415065548.1 SCO family protein -
  DXE44_RS08555 cyoE 1650868..1651761 (-) 894 WP_114654060.1 heme o synthase -
  DXE44_RS08560 - 1651758..1652888 (-) 1131 WP_114654061.1 COX15/CtaA family protein -
  DXE44_RS08565 - 1652885..1653544 (-) 660 WP_114654062.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 31519.21 Da        Isoelectric Point: 7.4781

>NTDB_id=1145194 DXE44_RS08540 WP_114654058.1 1648313..1649203(+) (pilA) [Polynucleobacter necessarius isolate PPGSP5]
MFGLRKTLGSLFKSNQTDEAWFDTLEESLILSDVGLPTTEQLISKLRKAAKSEKASSPEDLKQLLIQEVASLLKPLEPSP
NPLYVHEQKTTPEIWLVIGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLLEWGGRNQVDVIMQESGDAAA
VAHDAIHAAVSRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHQTLLVLDGNTGQNSLSQVKAFHAALGLSA
LIVTKLDGTTKGGVICALAHTLNDGSKPAVLALGKGEGINDLAPFTAAQYSSELFN

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1145194 DXE44_RS08540 WP_114654058.1 1648313..1649203(+) (pilA) [Polynucleobacter necessarius isolate PPGSP5]
ATGTTCGGCTTACGTAAAACCCTCGGATCCCTATTCAAATCGAATCAGACTGATGAAGCCTGGTTTGATACCCTAGAAGA
ATCTCTCATTCTGAGTGATGTGGGTCTGCCTACGACTGAACAACTGATTAGCAAACTTCGTAAGGCTGCCAAATCAGAAA
AGGCTAGTAGTCCTGAAGACTTGAAGCAACTACTCATTCAAGAGGTGGCAAGCCTCTTAAAGCCTTTGGAGCCAAGCCCG
AACCCTTTATATGTGCATGAACAAAAGACCACCCCGGAAATATGGCTAGTGATTGGCGTCAATGGCGCTGGTAAGACCAC
CACGATCGGCAAGCTCTGCAAACTCTTTCAGTCTCAAGGTAAATCCGTCTTGCTAGCAGCAGGCGATACTTTCCGAGCGG
CTGCACGCAATCAACTCCTAGAATGGGGTGGCCGCAACCAAGTTGACGTCATCATGCAAGAAAGCGGTGATGCTGCAGCC
GTGGCGCATGATGCGATTCATGCGGCGGTGTCTCGCAAGAGCGATATTTTGATTATTGATACTGCCGGCAGATTAGCCAC
TCAAGACCATCTCATGGAAGAATTGAAGAAGGTTAAAAGAGTGATTGGCAAGGCCCTTCCTGGGGCCCCTCACCAGACTT
TGCTAGTTCTTGATGGCAATACGGGTCAAAACAGTTTAAGCCAAGTAAAGGCCTTTCATGCTGCTTTAGGGCTGTCCGCC
TTAATCGTGACTAAGTTAGATGGCACTACCAAAGGTGGAGTGATCTGCGCACTGGCTCACACCCTCAATGATGGGTCAAA
ACCAGCAGTTTTAGCCCTTGGCAAAGGTGAGGGAATTAATGATTTAGCCCCCTTCACTGCCGCCCAATATTCATCCGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(4-68)

SRP54-type protein, GTPase domain (SRP54)

(93-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.56

98.986

0.52

Multiple sequence alignment