Detailed information NTG1144962

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BGLY_RS06115
• Genome accession: NZ_LT603683
• Coordinates: 1220088..1223780 (+)
• Length: 1230 a.a.
• NCBI ID: WP_046131291.1
• Uniprot ID: A0AAE2JVM9
• Organism: Bacillus glycinifermentans isolate BGLY
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1215088..1228780

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BGLY_RS06105 (BGLY_1183)	modB	1215836..1216492 (+)	657	WP_046131293.1	molybdate ABC transporter permease subunit	-
BGLY_RS06110 (BGLY_1184)	addB	1216601..1220101 (+)	3501	WP_046131292.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BGLY_RS06115 (BGLY_1185)	addA	1220088..1223780 (+)	3693	WP_046131291.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BGLY_RS06120 (BGLY_1186)	sbcD	1223848..1225032 (+)	1185	WP_046131290.1	exonuclease subunit SbcD	-
BGLY_RS06125 (BGLY_1187)	sbcC	1225029..1228421 (+)	3393	WP_046131289.1	exonuclease subunit SbcC	-
BGLY_RS06130	-	1228480..1228698 (-)	219	WP_046131288.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141344.16 Da
• Isoelectric Point: 5.4779

>NTDB_id=1144962 BGLY_RS06115 WP_046131291.1 1220088..1223780(+) (addA) [Bacillus glycinifermentans isolate BGLY]
METAKPKNSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKKLAENPGSLHLRRQLSLLNRASISTLHSFCLQVVRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYQKGDPA
FFELVDRYTTDRHDLDLQYLIKRVYEFSRSHPDPEAWLEKFMNLYDADPGAKIESLPFYSYIREDIALVLNSVREKLTRA
LELTKRPGGPAPRAENFLDDLAQIDKLLEHQDDFEALYELVPAVSFQRAKPCKGDDFDPSLIDEATDLRNSAKKMLEKLK
GDYFTRTPEQHLESLKEMKPVIRTLVQLVAEFGRRYRAAKKEKAIVDFSDLEHDCLAILTKKNEGGTTEPSEAAAYYRHL
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVVYDEQAELKLGASYPPNESTKTEVLIIENGEDAADEEAEELETVQLEARAVAGRVREL
ISGKFQVYDGKTKTTRNIQYRDIVILLRSMPWAPQFMDEFKQQGIPVYANLSTGYFEATEVAVTLSLLKVIDNAYQDIPL
ASVLRSPIVGLDENELSLIRIRDKKAPFYEALKAYMASADRDDPLYEKLARFDGFLKKWRAYAKNHSVAELIWEVYRDTK
YIDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFLVG
TAKDKDKLLAKWRTSASHAEWLLPDFERFQAKSYLDFIGPALMRHRDMADGDLNLPNDDIRQHPSRFTISWISAGELQAE
DRAQTEEEKHERLSLVKKGLPIDGAFDYEKEVRERLTWSYPFRDASQVRTKQSVSEIKRQKEYEDEYGDRSLIRPANETL
LFRRPSFMMAKGLTAAERGTAMHTVMQHLPLTRVPEKDELGRLLDMLIEKELLTEEQRAAIEEDDILAFFDTDIGGKLLG
ARRVEREVPFNMTLPAREVYPGFKTADDPVLIQGIIDCLFETDDGFYLLDYKTDRIHGKFQNGFEGAEPILRKRYETQIN
LYARAVETMLKTPLRGKALYFFDGGHVILF

Nucleotide

• Length: 3693 bp

>NTDB_id=1144962 BGLY_RS06115 WP_046131291.1 1220088..1223780(+) (addA) [Bacillus glycinifermentans isolate BGLY]
ATGGAAACGGCTAAGCCGAAAAACAGCACATGGACGGACGACCAATGGAAAGCGATCGTCTCATCCGGCCGGGATATTTT
GGTCGCCGCTGCGGCGGGCTCCGGAAAAACGGCCGTCCTTGTCGAGCGGATCATCCGAAAGATTACGGCAGAGGAAAATC
CGATCGATGTCGACCGGCTGCTCGTCGTCACCTTTACGAATGCATCGGCGGCGGAGATGAAACACCGGATCGGTGAAGCG
CTTGAGAAAAAGCTCGCCGAAAACCCCGGCTCCCTTCATTTGCGCAGGCAGCTTTCCTTGCTGAACAGGGCGAGCATTTC
GACATTGCATTCTTTTTGTCTTCAGGTCGTCCGCAAGTATTATTATATGATCGACGTTGATCCGGCTTTTCGGATCGCCG
ATCAGACAGAAGGCGAGCTGCTCGGCGATGAGGTCCTTGACGAGCTGTTTGAAGAAGAATATCAAAAAGGCGACCCGGCT
TTTTTTGAGCTGGTGGACAGATATACGACAGACCGCCACGATCTCGATTTGCAGTACCTTATCAAACGGGTTTACGAGTT
TTCCCGCTCACATCCCGACCCGGAAGCGTGGCTTGAAAAATTTATGAACCTGTACGACGCAGATCCCGGCGCGAAGATCG
AGTCGCTGCCGTTTTATTCCTACATCAGGGAAGATATCGCGCTTGTGTTGAACAGTGTGCGGGAAAAGCTGACCCGCGCG
CTTGAGCTGACAAAACGGCCGGGGGGGCCGGCTCCGCGCGCTGAAAACTTTCTCGACGATTTGGCGCAGATCGACAAGCT
GCTTGAGCATCAGGATGATTTTGAGGCCCTTTATGAGCTTGTGCCGGCTGTTTCGTTTCAGCGGGCCAAACCGTGCAAAG
GGGATGATTTCGATCCGTCTTTAATCGACGAAGCGACAGACCTTCGGAACAGTGCAAAAAAAATGCTTGAAAAGCTGAAA
GGCGACTATTTCACCCGAACGCCGGAGCAGCATTTGGAGAGTTTGAAGGAGATGAAGCCCGTCATCAGGACGCTTGTCCA
GCTCGTCGCAGAGTTCGGCAGACGCTATAGAGCGGCGAAGAAAGAAAAAGCCATCGTCGACTTCTCCGATCTTGAGCACG
ACTGCCTGGCGATTTTGACAAAGAAAAACGAAGGCGGGACAACGGAGCCGAGCGAAGCTGCGGCCTATTACCGGCACTTG
TTCCACGAAGTGCTCGTCGATGAGTATCAGGATACAAACCTTGTACAGGAGGCGATTTTAAAGCTTGTCGCCAAGGAGGA
GCACGAAGGAAACCTTTTTATGGTGGGCGATGTCAAGCAGTCGATCTACCGTTTCCGGCTGGCTGAGCCGCTTTTGTTTC
TTTCGAAATACAAGCGGTTCACAGATGACGGTTCAGGCGCCGGACAGAAAATCGATTTGAACAAAAACTTCAGAAGCCGG
TCCGACATTTTGGATAGCACCAATTTTTTGTTTAAACAGCTGATGGGGGAGAAGGTCGGCGAGGTTGTCTATGACGAACA
AGCCGAATTAAAGCTCGGCGCATCGTATCCTCCCAATGAGAGCACAAAAACAGAGGTGCTGATCATTGAAAATGGGGAAG
ATGCGGCTGATGAAGAGGCAGAAGAGCTTGAAACGGTTCAGCTTGAGGCAAGGGCTGTCGCCGGACGGGTCAGAGAGCTG
ATTTCCGGCAAATTTCAAGTGTATGACGGCAAAACGAAAACGACAAGAAACATCCAGTACCGCGACATCGTGATTTTGCT
GCGCTCCATGCCGTGGGCGCCGCAATTCATGGACGAGTTCAAGCAGCAGGGGATTCCGGTATACGCGAATCTGTCAACCG
GATATTTCGAAGCGACAGAAGTAGCCGTTACGCTTTCACTGCTTAAAGTCATCGACAATGCCTACCAGGATATTCCGCTT
GCATCCGTCCTCAGGTCACCGATTGTCGGCCTTGATGAAAACGAACTGTCCCTGATCAGAATCCGGGATAAAAAAGCGCC
GTTCTATGAAGCGCTGAAGGCGTATATGGCGTCAGCGGACAGGGATGATCCGCTTTATGAAAAGCTTGCCCGCTTTGACG
GGTTCCTGAAGAAGTGGAGGGCATACGCAAAAAACCATTCCGTGGCAGAGCTGATATGGGAAGTTTACCGCGATACGAAA
TATATCGATTATGTCGGCGGAATGCCGGGCGGAAAGCAGCGGCAAGCCAATTTGCGCGCGCTCTATGACAGGGCGAGATC
GTACGAATCGACCTCCTTCCGCGGATTGTTCCGCTTTTTGCGATTTATTGAACGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCGCGGGCTTTGAGCGAACAGGAAGACGTCGTCCGCCTGATGACGATCCATTCGAGCAAAGGCCTTGAATTTCCC
GTCGTATTCACGGCGGGCCTCGGCCGGAATTTCAATATGATGGATCTCAACAAATCGTATCTGCTTGATAAAGAGCTCGG
GTTCGGGACGAAATTTATCCATCCGAAATGGCGCATCAGCTATCCGACGCTGCCGCTTGTGGCGATGAAGAAAAAAATGC
GGCGCGAGCTTTTATCAGAAGAGCTCCGCGTCTTGTATGTCGCTCTCACCCGTGCAAAGGAAAAACTCTTTTTGGTCGGA
ACGGCGAAAGACAAGGACAAGCTTTTGGCAAAATGGCGGACCTCCGCCTCCCATGCCGAATGGCTGCTTCCTGATTTCGA
ACGCTTTCAGGCGAAATCGTATCTCGATTTTATCGGCCCAGCTTTAATGCGGCACAGGGATATGGCAGACGGTGATCTGA
ATTTGCCGAATGATGACATCCGTCAGCATCCGTCCCGTTTTACGATCAGCTGGATATCAGCAGGAGAGCTGCAGGCCGAA
GATAGAGCGCAGACTGAAGAAGAAAAACACGAACGGCTGTCACTGGTAAAAAAAGGGCTGCCGATCGATGGGGCTTTCGA
TTATGAAAAAGAAGTCCGCGAACGGCTGACATGGTCGTATCCTTTCCGGGATGCGTCACAGGTCCGCACAAAACAGTCTG
TCTCCGAAATCAAGAGACAGAAGGAGTACGAGGATGAGTACGGCGACCGTTCATTGATTCGTCCGGCAAATGAAACTTTG
CTGTTCAGAAGACCCTCTTTCATGATGGCAAAAGGGCTGACCGCCGCTGAGCGGGGAACGGCAATGCATACAGTCATGCA
GCATCTTCCGCTCACTCGCGTCCCCGAAAAGGATGAGCTCGGCCGTCTGCTGGACATGCTTATCGAAAAAGAGCTTCTAA
CGGAAGAGCAACGCGCAGCTATCGAAGAAGATGACATTCTCGCGTTTTTTGATACGGATATAGGCGGAAAACTGCTTGGC
GCCCGCCGGGTTGAGCGGGAAGTGCCCTTCAACATGACGCTGCCTGCACGAGAGGTTTATCCAGGCTTCAAGACTGCCGA
TGACCCCGTCTTAATTCAAGGGATTATCGACTGCTTGTTCGAAACCGATGACGGCTTTTATTTGCTTGACTACAAAACCG
ACCGGATACACGGCAAATTTCAAAACGGCTTTGAAGGAGCGGAGCCGATTTTACGAAAACGCTACGAGACGCAGATCAAC
CTTTACGCCCGGGCTGTCGAAACGATGCTTAAGACGCCTCTGAGAGGAAAGGCTCTTTACTTTTTCGACGGCGGGCACGT
GATCCTGTTTTGA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	73.74	100	0.737