Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LLA12_RS11465 Genome accession   NZ_LT599049
Coordinates   2346677..2348038 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain A12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2341677..2353038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLA12_RS11430 (LLA12_02424) - 2342226..2342720 (+) 495 WP_021723080.1 GNAT family N-acetyltransferase -
  LLA12_RS11435 (LLA12_02425) - 2342811..2343080 (-) 270 WP_021723079.1 DUF1048 domain-containing protein -
  LLA12_RS14230 - 2343120..2343467 (-) 348 Protein_2251 DUF1048 domain-containing protein -
  LLA12_RS11445 (LLA12_02428) - 2343454..2343816 (-) 363 WP_021723076.1 PadR family transcriptional regulator -
  LLA12_RS11450 (LLA12_02429) - 2343937..2344566 (-) 630 WP_021723075.1 hypothetical protein -
  LLA12_RS11455 (LLA12_02430) - 2344631..2345716 (-) 1086 WP_021723074.1 PIN/TRAM domain-containing protein -
  LLA12_RS11460 (LLA12_02432) - 2345947..2346633 (+) 687 WP_050573393.1 YoaK family protein -
  LLA12_RS11465 (LLA12_02433) radA 2346677..2348038 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  LLA12_RS11475 (LLA12_02435) rplQ 2348665..2349045 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  LLA12_RS11480 (LLA12_02436) - 2349064..2350002 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  LLA12_RS11485 (LLA12_02437) rpsK 2350050..2350433 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  LLA12_RS11490 (LLA12_02438) rpsM 2350454..2350819 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  LLA12_RS11495 (LLA12_02439) rpmJ 2350837..2350953 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  LLA12_RS11500 (LLA12_02440) infA 2351007..2351225 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  LLA12_RS11505 (LLA12_02441) - 2351522..2352169 (-) 648 WP_010906298.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=1144883 LLA12_RS11465 WP_003130563.1 2346677..2348038(-) (radA) [Lactococcus lactis subsp. lactis strain A12]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1144883 LLA12_RS11465 WP_003130563.1 2346677..2348038(-) (radA) [Lactococcus lactis subsp. lactis strain A12]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACTTAGATGAATTTAATCGTGTA
TTAGGAGGGGGTGTTGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGTCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTGGGAGATATTGACCGAGACTTCTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATTATGACACCAGAAATTCAATCAACTCAAGGCTCAGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACAAATGATATTGCGACCTTTATTGTTGGTCATG
TGACTAAAGAAGGTCAGCTGGCTGGGCCAAGGATGTTAGAGCATATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCCTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCCATATTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTTTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTCGCGGTCGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTGAATGAAGCTGCAAAACTAGGGTTTAAAAAAGTATATGCTCCAAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment