Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   PCPL58_RS26455 Genome accession   NZ_LT222319
Coordinates   5412759..5414978 (+) Length   739 a.a.
NCBI ID   WP_003365984.1    Uniprot ID   -
Organism   Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5407759..5419978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCPL58_RS26440 (PCPL58_4833) - 5410100..5411368 (-) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -
  PCPL58_RS26445 (PCPL58_4834) - 5411551..5412354 (-) 804 WP_065350826.1 thermonuclease family protein -
  PCPL58_RS26450 (PCPL58_4835) rpmE 5412368..5412589 (-) 222 WP_002551648.1 50S ribosomal protein L31 -
  PCPL58_RS26455 (PCPL58_4836) priA 5412759..5414978 (+) 2220 WP_003365984.1 primosomal protein N' Machinery gene
  PCPL58_RS26460 (PCPL58_4837) argS 5415076..5416812 (+) 1737 WP_003432149.1 arginine--tRNA ligase -
  PCPL58_RS26465 (PCPL58_4838) - 5416814..5417509 (+) 696 WP_002551645.1 SPOR domain-containing protein -
  PCPL58_RS26470 (PCPL58_4839) hslV 5417716..5418246 (+) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  PCPL58_RS26475 (PCPL58_4840) hslU 5418313..5419650 (+) 1338 WP_003365974.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81870.19 Da        Isoelectric Point: 8.5462

>NTDB_id=1143663 PCPL58_RS26455 WP_003365984.1 5412759..5414978(+) (priA) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1143663 PCPL58_RS26455 WP_003365984.1 5412759..5414978(+) (priA) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCAATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCCGCG
CTGTTCAAACTGTGCCTGTGGACCTCGCAGTATTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAGGCCCTGACCACCCTTGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTCAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAACTGGTGTACGTCGAGGTCCGCAGCCATGC
ACCCAGCGCACGCCATGAGCACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTCCTGCTGGCCGGCGTCACTGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAGACCCTCGAAGCGGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGGCTG
ATCATCATCGACGAGGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCTTGGAAAGCCTGCATAACGCCTACACCG
GCCGCTACGGCCTGCTGCGCCTGAATCAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGTCAGACGCTCGCGGCAGGCCAGCAGGTATTGGT
ATTCCTCAACCGTCGCGGCTTTGCACCCACCTTGCTGTGCCACGACTGCGGGTGGATGTCCGGCTGCCAGCGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGTGTACCGCGCCAGTGT
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGCCAGCCATGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTATCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTGCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAACCTGGCAAGGTGATCATCCAGACCCACTTGGCCGACCATCCACTGCTGATTCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
GGTTGCGCAGATGGCGCTGGGCGGCATTGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGACGTTATC
GCGCGCAGTTGCTGGTGCAGTCCAGCGCCCGTGCGCCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGCCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment