Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ECLTO80_RS21100 Genome accession   NZ_LS999206
Coordinates   4288864..4290297 (+) Length   477 a.a.
NCBI ID   WP_133983259.1    Uniprot ID   -
Organism   Enterobacter hormaechei isolate EC-TO80     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4283864..4295297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECLTO80_RS21070 (ECLTO80_04240) - 4284431..4285408 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  ECLTO80_RS21075 (ECLTO80_04241) kdsD 4285422..4286408 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  ECLTO80_RS21080 (ECLTO80_04242) kdsC 4286423..4286989 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ECLTO80_RS21085 (ECLTO80_04243) lptC 4286986..4287561 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  ECLTO80_RS21090 (ECLTO80_04244) lptA 4287530..4288084 (+) 555 WP_062938089.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ECLTO80_RS21095 (ECLTO80_04245) lptB 4288091..4288816 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  ECLTO80_RS21100 (ECLTO80_04246) rpoN/rpoN1 4288864..4290297 (+) 1434 WP_133983259.1 RNA polymerase factor sigma-54 Machinery gene
  ECLTO80_RS21105 (ECLTO80_04247) hpf 4290320..4290607 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  ECLTO80_RS21110 (ECLTO80_04248) ptsN 4290690..4291181 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ECLTO80_RS21115 (ECLTO80_04249) rapZ 4291227..4292081 (+) 855 WP_003861870.1 RNase adapter RapZ -
  ECLTO80_RS21120 (ECLTO80_04250) npr 4292078..4292350 (+) 273 WP_133983261.1 PTS phosphocarrier protein NPr -
  ECLTO80_RS21125 (ECLTO80_04251) mtgA 4292390..4293115 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ECLTO80_RS21130 (ECLTO80_04252) elbB 4293112..4293765 (-) 654 WP_032655241.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54100.00 Da        Isoelectric Point: 4.3617

>NTDB_id=1143464 ECLTO80_RS21100 WP_133983259.1 4288864..4290297(+) (rpoN/rpoN1) [Enterobacter hormaechei isolate EC-TO80]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEKKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1143464 ECLTO80_RS21100 WP_133983259.1 4288864..4290297(+) (rpoN/rpoN1) [Enterobacter hormaechei isolate EC-TO80]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAAAAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCTGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATTTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment