Detailed information NTG1142538

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: DQL77_RS00330
• Genome accession: NZ_LS483484
• Coordinates: 64420..65769 (-)
• Length: 449 a.a.
• NCBI ID: WP_000815654.1
• Uniprot ID: Q4JFB2
• Organism: Staphylococcus aureus strain NCTC13277
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	44403..65769	64420..65769	within	0

Gene organization within MGE regions

Location: 44403..65769

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL77_RS00210 (NCTC13277_00041)	mecA	44403..46412 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
DQL77_RS00215 (NCTC13277_00042)	mecR1	46509..48266 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
DQL77_RS00220 (NCTC13277_00043)	mecI	48266..48610 (+)	345	WP_000369213.1	mecA-type methicillin resistance repressor MecI	-
DQL77_RS00225	psm-mec	48722..48790 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
DQL77_RS00230 (NCTC13277_00044)	-	49124..50254 (+)	1131	WP_111764890.1	ROK family transcriptional regulator	-
DQL77_RS00240 (NCTC13277_00046)	cstB	50368..51702 (-)	1335	Protein_40	persulfide dioxygenase-sulfurtransferase CstB	-
DQL77_RS00245 (NCTC13277_00047)	-	51735..52799 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
DQL77_RS00250 (NCTC13277_00048)	cstR	52935..53195 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
DQL77_RS00255 (NCTC13277_00049)	-	53195..53833 (+)	639	Protein_43	sulfite exporter TauE/SafE family protein	-
DQL77_RS00265 (NCTC13277_00050)	-	54178..54840 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
DQL77_RS00280 (NCTC13277_00051)	erm(A)	55374..56105 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
DQL77_RS00285 (NCTC13277_00052)	-	56231..57013 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
DQL77_RS00290 (NCTC13277_00053)	-	57164..57541 (-)	378	WP_000361059.1	DUF6262 family protein	-
DQL77_RS00295 (NCTC13277_00054)	-	57548..59441 (-)	1894	Protein_48	site-specific integrase	-
DQL77_RS00300 (NCTC13277_00056)	-	59438..60523 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
DQL77_RS00305 (NCTC13277_00057)	-	60642..60959 (-)	318	WP_000659050.1	JAB domain-containing protein	-
DQL77_RS00310 (NCTC13277_00058)	-	60980..61486 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
DQL77_RS00315 (NCTC13277_00059)	-	61504..61815 (-)	312	WP_000700853.1	DUF960 family protein	-
DQL77_RS00320 (NCTC13277_00061)	-	61902..62252 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
DQL77_RS00325 (NCTC13277_00062)	ccrB	62770..64398 (-)	1629	WP_001186599.1	recombinase family protein	Machinery gene
DQL77_RS00330 (NCTC13277_00063)	ccrA	64420..65769 (-)	1350	WP_000815654.1	recombinase family protein	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52665.36 Da
• Isoelectric Point: 10.0809

>NTDB_id=1142538 DQL77_RS00330 WP_000815654.1 64420..65769(-) (ccrA) [Staphylococcus aureus strain NCTC13277]
MKQVIGYLRQSTMKQQSLAAQKQAIEAITEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQKQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQNTIQ
NIIQKRFTLNMLYPYIDEILITKSKTLMGIYFKNEPLNIVNQTTQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=1142538 DQL77_RS00330 WP_000815654.1 64420..65769(-) (ccrA) [Staphylococcus aureus strain NCTC13277]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAACCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTGCATGATGGTTATTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATTATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAAAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAAAACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATGCTGTATCCCTACATTGATGAAATTCTTATCACAAAAAGTAAAACACT
AATGGGTATCTATTTCAAAAATGAACCCTTAAACATCGTCAATCAAACAACGCAATCGTCAATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-248)

• Resolvase, N terminal domain (Resolvase) (4-147)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

3D structure

• AlphaFold DB: Q4JFB2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	95.323	100	0.953
ccrA	Staphylococcus aureus COL	75.501	100	0.755