Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQL46_RS11270 Genome accession   NZ_LS483482
Coordinates   2352922..2354289 (-) Length   455 a.a.
NCBI ID   WP_002459953.1    Uniprot ID   A0A853V434
Organism   Staphylococcus lugdunensis strain NCTC12217     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2347922..2359289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL46_RS11255 (NCTC12217_02285) cysE 2349035..2349682 (-) 648 WP_172412359.1 serine O-acetyltransferase -
  DQL46_RS11260 (NCTC12217_02287) gltX 2350039..2351496 (-) 1458 WP_002459951.1 glutamate--tRNA ligase -
  DQL46_RS11265 (NCTC12217_02288) - 2351820..2352887 (-) 1068 WP_002459952.1 PIN/TRAM domain-containing protein -
  DQL46_RS11270 (NCTC12217_02289) radA 2352922..2354289 (-) 1368 WP_002459953.1 DNA repair protein RadA Machinery gene
  DQL46_RS11275 (NCTC12217_02290) - 2354520..2356979 (-) 2460 WP_002459954.1 ATP-dependent Clp protease ATP-binding subunit -
  DQL46_RS11280 (NCTC12217_02291) - 2356993..2358006 (-) 1014 WP_070647769.1 protein arginine kinase -
  DQL46_RS11285 (NCTC12217_02292) - 2357999..2358556 (-) 558 WP_002459956.1 UvrB/UvrC motif-containing protein -
  DQL46_RS11290 (NCTC12217_02293) - 2358583..2359044 (-) 462 WP_002459957.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50027.45 Da        Isoelectric Point: 7.0479

>NTDB_id=1142532 DQL46_RS11270 WP_002459953.1 2352922..2354289(-) (radA) [Staphylococcus lugdunensis strain NCTC12217]
MAKKKIIFECMACGYQSPKWMGKCPNCGAWNQMEEIIEQKAAHPKHGVKAQTATGKVQKLNSIQHESTPRVQTKSQEFNR
VLGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQQLNVLYITGEESLNQTKLRAERLEEDSSQLNVLAETDLEVIHQTV
SETKPDILVVDSIQTIYHPEIASAPGSVSQVRESTQSLMNIAKQMDIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLSVIVATASSFKDEAVDGLDCYIGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVILPKTNIGGWTFPDNIQVIGVSTVHEALKYAFKA

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1142532 DQL46_RS11270 WP_002459953.1 2352922..2354289(-) (radA) [Staphylococcus lugdunensis strain NCTC12217]
GTGGCCAAGAAGAAAATCATTTTTGAGTGTATGGCATGTGGCTATCAATCTCCTAAATGGATGGGTAAATGTCCTAATTG
TGGTGCTTGGAACCAAATGGAAGAGATTATAGAACAGAAGGCTGCACATCCTAAACATGGGGTTAAAGCACAAACGGCTA
CCGGAAAAGTTCAAAAATTAAACAGTATTCAACATGAATCGACACCACGTGTGCAAACGAAATCTCAAGAGTTTAATCGT
GTGTTAGGTGGGGGGATTGTTAGTGGTTCTTTAGTGTTGATTGGTGGAGATCCAGGTATCGGGAAATCAACACTATTACT
ACAAATCTGTGCCTCGCTTTCGCAACAATTAAACGTGCTGTATATTACAGGTGAGGAATCATTAAATCAAACAAAATTGA
GAGCAGAACGCTTAGAGGAAGACTCTAGTCAATTAAATGTGTTAGCTGAGACAGATTTAGAAGTTATTCACCAAACCGTT
TCAGAAACTAAGCCAGATATTTTAGTGGTGGACTCTATTCAGACGATTTACCATCCTGAGATTGCATCGGCACCCGGATC
TGTTTCTCAAGTGCGTGAGAGTACTCAAAGTTTGATGAATATTGCGAAGCAGATGGATATTGCCACATTTATTGTAGGAC
ATGTGACTAAAGAGGGGCAGATAGCTGGTCCACGATTGTTAGAACACATGGTAGATACAGTGCTTTATTTTGAAGGTGAT
GAACATCATGCGTATCGTATTTTACGTGCTGTTAAAAACCGTTTTGGTTCAACAAATGAAATGGGTATTTTCGAAATGAA
GCAAAGTGGTTTGAAAGGTGTCAAAAACCCGTCCGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCGGGTTCAACAA
TTGTGTCGACAATGGAAGGAACACGACCATTACTTATTGAAGTACAAGCTTTAGTGACCCCAACAACGTTTAATAATCCA
CGACGTATGGCAACAGGTATTGACCATAACCGTCTGAGCCTGCTTATGGCAGTGCTTGAAAAGAAAGAAAATTATTTATT
GCAGCAACAAGATGCATATATTAAAGTGGCAGGTGGTGTAAGATTAACAGAACCGGCTGTAGACTTAAGTGTAATTGTAG
CAACTGCATCAAGCTTTAAAGATGAAGCGGTTGATGGTTTAGATTGTTATATTGGAGAAGTAGGTTTGACTGGTGAAGTC
AGACGTGTGTCACGTATTGAACAACGCGTTCAAGAAGCAGCTAAACTAGGATTTAAACGTGTGATACTCCCTAAAACAAA
TATCGGCGGATGGACATTTCCTGACAATATACAAGTCATAGGTGTTTCGACAGTACATGAGGCATTGAAATATGCTTTCA
AAGCTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(68-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A853V434

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.502

100

0.659
  radA Streptococcus pneumoniae Rx1

59.603

99.56

0.593
  radA Streptococcus pneumoniae D39

59.603

99.56

0.593
  radA Streptococcus pneumoniae R6

59.603

99.56

0.593
  radA Streptococcus pneumoniae TIGR4

59.603

99.56

0.593
  radA Streptococcus mitis NCTC 12261

59.603

99.56

0.593
  radA Streptococcus mitis SK321

59.603

99.56

0.593

Multiple sequence alignment