Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQN28_RS01135 Genome accession   NZ_LS483430
Coordinates   197788..199149 (+) Length   453 a.a.
NCBI ID   WP_020904882.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC12044     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 192788..204149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN28_RS01120 (NCTC12044_00220) - 194482..196266 (+) 1785 WP_002986115.1 ABC transporter ATP-binding protein -
  DQN28_RS01125 (NCTC12044_00221) - 196384..197151 (+) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  DQN28_RS01130 (NCTC12044_00223) - 197261..197707 (+) 447 WP_002986111.1 dUTP diphosphatase -
  DQN28_RS01135 (NCTC12044_00224) radA 197788..199149 (+) 1362 WP_020904882.1 DNA repair protein RadA Machinery gene
  DQN28_RS01140 (NCTC12044_00225) - 199338..199835 (+) 498 WP_010921867.1 beta-class carbonic anhydrase -
  DQN28_RS01145 (NCTC12044_00226) - 199966..200676 (+) 711 WP_002991188.1 TIGR00266 family protein -
  DQN28_RS01150 (NCTC12044_00227) gltX 200858..202303 (+) 1446 WP_111686159.1 glutamate--tRNA ligase -
  DQN28_RS01155 (NCTC12044_00228) - 202698..204044 (+) 1347 WP_002991186.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49416.66 Da        Isoelectric Point: 5.8210

>NTDB_id=1141250 DQN28_RS01135 WP_020904882.1 197788..199149(+) (radA) [Streptococcus pyogenes strain NCTC12044]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVDQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1141250 DQN28_RS01135 WP_020904882.1 197788..199149(+) (radA) [Streptococcus pyogenes strain NCTC12044]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCTACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGCGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCTTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGATCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(56-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.651

100

0.857
  radA Streptococcus pneumoniae D39

85.43

100

0.854
  radA Streptococcus pneumoniae R6

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.43

100

0.854
  radA Streptococcus mitis SK321

85.43

100

0.854
  radA Streptococcus pneumoniae TIGR4

85.43

100

0.854
  radA Bacillus subtilis subsp. subtilis str. 168

61.369

100

0.614

Multiple sequence alignment