Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQN51_RS03075 Genome accession   NZ_LS483405
Coordinates   672703..674079 (+) Length   458 a.a.
NCBI ID   WP_042516665.1    Uniprot ID   -
Organism   Levilactobacillus brevis strain NCTC13768     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 667703..679079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN51_RS03050 (NCTC13768_00625) - 667728..668960 (+) 1233 WP_024525649.1 serine hydrolase -
  DQN51_RS03055 (NCTC13768_00626) - 669094..670428 (-) 1335 WP_035464379.1 aminopeptidase C -
  DQN51_RS12385 (NCTC13768_00627) - 670574..670744 (+) 171 WP_021741480.1 hypothetical protein -
  DQN51_RS03060 (NCTC13768_00628) rpiA 670855..671541 (-) 687 WP_011667345.1 ribose-5-phosphate isomerase RpiA -
  DQN51_RS03065 (NCTC13768_00629) - 671549..671890 (-) 342 WP_021741479.1 hypothetical protein -
  DQN51_RS03070 (NCTC13768_00630) - 672069..672608 (+) 540 WP_021741478.1 dUTP diphosphatase -
  DQN51_RS03075 (NCTC13768_00631) radA 672703..674079 (+) 1377 WP_042516665.1 DNA repair protein RadA Machinery gene
  DQN51_RS03080 (NCTC13768_00632) - 674115..675299 (+) 1185 WP_021741476.1 PIN/TRAM domain-containing protein -
  DQN51_RS03085 (NCTC13768_00633) gltX 675563..677053 (+) 1491 WP_011667350.1 glutamate--tRNA ligase -
  DQN51_RS03090 (NCTC13768_00634) cysS 677208..678620 (+) 1413 WP_021741474.1 cysteine--tRNA ligase -
  DQN51_RS03095 (NCTC13768_00635) - 678620..679027 (+) 408 WP_011667352.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49758.07 Da        Isoelectric Point: 7.0881

>NTDB_id=1140388 DQN51_RS03075 WP_042516665.1 672703..674079(+) (radA) [Levilactobacillus brevis strain NCTC13768]
MAKPRTTYVCQNCDYSSPRYLGRCPNCGEWNTMVEEVVTPAAAKPQSTRTTASGERSHPQLMSEIKHSTESRTKTQMEEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSQTGGKVLYVSGEESASQIKMRADRLVVNSDHLYLYPETDMASIR
ANIEQMKPDYVVIDSVQTMQAPGIESAIGSVSQIRAVTGELMQIAKTNGITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRAVKNRFGSTNELGIFEMREGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALITPSVF
GNAQRTSSGLDRNRVSLLMAVLEKRANLMLQNQDAFLKAAGGVKLDEPAIDLAIALSIASSYRDTATQPTDCFVGEVGLT
GEIRRVNRIEQRVAEAKKLGFKRIFIPKNNLQGWTPDPGIDVVGVSTLRQALKLALDV

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1140388 DQN51_RS03075 WP_042516665.1 672703..674079(+) (radA) [Levilactobacillus brevis strain NCTC13768]
GTGGCGAAACCAAGAACAACGTATGTGTGTCAAAATTGTGATTATAGCTCACCCCGTTATTTGGGGCGTTGTCCCAATTG
TGGTGAGTGGAACACGATGGTGGAAGAAGTCGTCACACCGGCTGCCGCCAAGCCACAATCAACCCGGACCACTGCTTCCG
GAGAACGGTCACACCCACAACTCATGAGTGAGATTAAACACTCAACGGAAAGCCGAACGAAGACGCAAATGGAAGAACTG
AACCGAGTGCTGGGTGGGGGAATTGTGCCGGGATCTTTGATCCTAATCGGTGGTGATCCTGGAATTGGAAAATCAACCCT
CTTGCTTCAAGTATCGGGGCAACTCAGCCAAACAGGCGGTAAAGTGTTGTATGTTTCTGGAGAAGAAAGTGCGTCACAGA
TTAAAATGCGGGCGGATCGGTTAGTCGTCAATAGCGACCACTTGTATCTTTATCCAGAAACTGATATGGCCAGTATTCGC
GCGAACATTGAACAGATGAAACCTGATTACGTGGTCATTGATTCCGTTCAGACCATGCAGGCGCCGGGAATCGAGTCGGC
GATTGGTTCGGTCTCACAAATTCGTGCGGTCACTGGTGAGCTGATGCAGATTGCCAAAACCAATGGCATCACTATTTTTG
TGGTGGGGCATGTGACGAAGGGCGGCGCAATTGCCGGTCCTAAGATTCTGGAACACATGGTAGATACGGTTCTTTACTTT
GAAGGTGACCTCCATCATACGTACCGAATTCTCCGAGCGGTCAAGAACCGATTCGGGTCAACGAATGAGTTGGGTATCTT
TGAAATGCGTGAAGGTGGCTTGTATGAAGTTGCTAATCCGTCAGAAATTTTCTTGGAAGAACGATTGAAGGACGCAACCG
GCTCGGCCATTGTGGTATCAATGGAAGGTACGCGGCCAATTTTGGTAGAAGTTCAGGCGTTAATTACACCATCGGTCTTT
GGTAATGCGCAACGCACATCCAGCGGCCTGGATCGTAATCGGGTGTCGTTGTTGATGGCTGTGCTAGAAAAACGGGCCAA
TCTAATGCTCCAAAATCAAGATGCATTTTTAAAGGCAGCAGGTGGCGTTAAGCTGGATGAACCGGCGATTGATTTGGCAA
TTGCACTGTCGATTGCATCGAGTTATCGCGATACGGCCACGCAGCCAACGGATTGTTTTGTCGGTGAAGTTGGCCTGACT
GGTGAAATCCGTCGGGTTAATCGAATTGAACAACGGGTTGCGGAAGCGAAAAAACTGGGCTTTAAGCGGATCTTCATTCC
CAAGAATAACCTTCAAGGGTGGACGCCCGATCCCGGAATTGATGTGGTCGGCGTGTCAACGTTACGACAAGCGTTAAAAC
TGGCTTTGGACGTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.667

98.908

0.659
  radA Streptococcus mitis NCTC 12261

66.446

98.908

0.657
  radA Streptococcus pneumoniae Rx1

66.446

98.908

0.657
  radA Streptococcus pneumoniae D39

66.446

98.908

0.657
  radA Streptococcus pneumoniae R6

66.446

98.908

0.657
  radA Streptococcus pneumoniae TIGR4

66.446

98.908

0.657
  radA Bacillus subtilis subsp. subtilis str. 168

64.254

99.563

0.64

Multiple sequence alignment