Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQM80_RS01060 Genome accession   NZ_LS483399
Coordinates   190338..191699 (+) Length   453 a.a.
NCBI ID   WP_111701765.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8227     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 185338..196699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM80_RS01045 (NCTC8227_00207) - 187030..188814 (+) 1785 WP_111701761.1 ABC transporter ATP-binding protein -
  DQM80_RS01050 (NCTC8227_00208) - 188932..189699 (+) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  DQM80_RS01055 (NCTC8227_00209) - 189809..190255 (+) 447 WP_111701763.1 dUTP diphosphatase -
  DQM80_RS01060 (NCTC8227_00210) radA 190338..191699 (+) 1362 WP_111701765.1 DNA repair protein RadA Machinery gene
  DQM80_RS01065 (NCTC8227_00211) - 191887..192384 (+) 498 WP_021340152.1 beta-class carbonic anhydrase -
  DQM80_RS01070 (NCTC8227_00212) - 192515..193225 (+) 711 WP_030127060.1 TIGR00266 family protein -
  DQM80_RS01075 (NCTC8227_00213) gltX 193407..194852 (+) 1446 WP_111701767.1 glutamate--tRNA ligase -
  DQM80_RS01080 (NCTC8227_00214) - 195246..196591 (+) 1346 Protein_173 GHKL domain-containing protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49367.66 Da        Isoelectric Point: 6.0944

>NTDB_id=1140212 DQM80_RS01060 WP_111701765.1 190338..191699(+) (radA) [Streptococcus pyogenes strain NCTC8227]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLTGEKSKPVKLKDVDNISYHRTQTDMAEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NLNPDFLIIDSIQTIISPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSAGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYAPKNALQGISIPNGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1140212 DQM80_RS01060 WP_111701765.1 190338..191699(+) (radA) [Streptococcus pyogenes strain NCTC8227]
ATAGCAAAGAAAAAAGCAACCTTCATTTGTCAAGAATGTGGGTATCAGTCACCCAAATATTTAGGGCGTTGCCCAAACTG
CTCGGCCTGGTCTTCCTTTGTGGAAGAAGTTGAGGTCAAAGAGGTTAAAAATGCTCGTGTCAGTTTGACAGGTGAGAAAA
GTAAGCCCGTTAAATTAAAAGATGTGGACAATATCAGCTATCATCGGACACAGACCGATATGGCAGAGTTTAACCGTGTC
CTTGGTGGCGGTGTCGTTCCGGGGAGCTTGATTTTAATTGGTGGGGATCCAGGTATTGGGAAGTCCACCTTGCTCTTGCA
GGTATCTACCCAGCTAGCTAATAAGGGAACTGTTCTGTATGTTTCTGGGGAAGAATCAGCAGAGCAGATTAAACTTCGGA
GTGAGCGTCTGGGGGATATTGACAATGAATTTTACCTCTATGCTGAAACCAATATGCAGGCCATTCGTACAGAGATTGAA
AACCTGAATCCAGATTTCTTGATTATTGATTCTATCCAAACCATTATAAGTCCTGATATTACAGGTGTTCAAGGATCAGT
GAGTCAGGTGAGAGAAGTCACTGCCGAATTGATGCAACTTGCAAAGACTAACAACATTGCAACCTTTATTGTAGGACATG
TTACCAAGGAAGGAACCTTAGCTGGTCCCCGTATGTTAGAACATATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCATACTTTTCGCATCTTGCGGGCGGTGAAAAACCGTTTTGGCTCTACCAACGAGATCGGCATTTTTGAAATGCAATC
AGCTGGACTTGTTGAGGTCTTAAACCCAAGCCAAGTTTTTCTTGAAGAACGATTAGATGGTGCTACAGGCTCCGCCATTG
TGGTGACCATGGAGGGTAGTCGTCCAATTTTAGCGGAAGTCCAATCTTTGGTAACCCCAACCGTTTTTGGTAATGCTAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATCATGGCAGTCTTAGAAAAACGCTGTGGGCTTCTCTTGCA
AAATCAAGATGCCTACCTCAAATCGGCTGGTGGTGTCAAGCTAGACGAACCTGCCATTGATTTAGCTGTAGCCGTAGCCA
TCGCATCCAGTTATAAAGAAAAACCAACCAACCCTCAGGAAGCCTTCTTAGGAGAGATTGGGTTGACTGGTGAGATTCGG
CGGGTGACCCGTATCGAACAACGAATTAATGAAGCAGCCAAGCTTGGTTTTACTAAAATTTACGCCCCTAAAAATGCTCT
GCAGGGCATCAGTATTCCTAATGGTATTGAAGTGGTGGGTGTGACAACAGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(57-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

86.313

100

0.863
  radA Streptococcus pneumoniae Rx1

86.093

100

0.861
  radA Streptococcus pneumoniae D39

86.093

100

0.861
  radA Streptococcus pneumoniae R6

86.093

100

0.861
  radA Streptococcus pneumoniae TIGR4

86.093

100

0.861
  radA Streptococcus mitis SK321

86.093

100

0.861
  radA Bacillus subtilis subsp. subtilis str. 168

62.252

100

0.623

Multiple sequence alignment