Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQM95_RS09170 Genome accession   NZ_LS483397
Coordinates   1786777..1788144 (-) Length   455 a.a.
NCBI ID   WP_015911975.1    Uniprot ID   B9DVX0
Organism   Streptococcus uberis strain NCTC3858     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1781777..1793144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM95_RS09150 (NCTC3858_01862) - 1782035..1783321 (-) 1287 WP_037592586.1 hypothetical protein -
  DQM95_RS09155 (NCTC3858_01863) gltX 1783582..1785027 (-) 1446 WP_046388361.1 glutamate--tRNA ligase -
  DQM95_RS09160 (NCTC3858_01864) - 1785211..1785924 (-) 714 WP_015911973.1 TIGR00266 family protein -
  DQM95_RS09165 (NCTC3858_01865) - 1786120..1786617 (-) 498 WP_015911974.1 carbonic anhydrase -
  DQM95_RS09170 (NCTC3858_01866) radA 1786777..1788144 (-) 1368 WP_015911975.1 DNA repair protein RadA Machinery gene
  DQM95_RS09175 (NCTC3858_01867) - 1788192..1788638 (-) 447 WP_037592584.1 dUTP diphosphatase -
  DQM95_RS09180 (NCTC3858_01868) - 1788754..1789113 (-) 360 WP_015911977.1 YbaN family protein -
  DQM95_RS09185 (NCTC3858_01869) - 1789302..1789904 (+) 603 WP_015911978.1 NADPH-dependent FMN reductase -
  DQM95_RS09190 (NCTC3858_01870) - 1789923..1791170 (+) 1248 WP_037592582.1 NAD(P)H-dependent oxidoreductase -
  DQM95_RS09195 (NCTC3858_01871) - 1791213..1791977 (-) 765 WP_037592581.1 epoxyqueuosine reductase QueH -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49734.21 Da        Isoelectric Point: 6.3763

>NTDB_id=1140184 DQM95_RS09170 WP_015911975.1 1786777..1788144(-) (radA) [Streptococcus uberis strain NCTC3858]
MAKKKATYVCQECGYQSPKYLGRCPNCSAWSSFIEEVEVKEVKNARVSLTGEKSRPVKLKDVDNINYARTKTDMEEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIAPDFLIIDSIQTIMSPEIQGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTIAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRISLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEMPTNPQEAFLGEIGLTGEIR
RVTRMEQRINEAAKLGFTKIYAPKNALHGIDIPKGIQVVGVTTVGEVLKAVFSKA

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1140184 DQM95_RS09170 WP_015911975.1 1786777..1788144(-) (radA) [Streptococcus uberis strain NCTC3858]
ATGGCTAAAAAGAAAGCGACATATGTTTGTCAGGAGTGTGGCTATCAATCCCCTAAATACCTTGGCAGATGTCCTAATTG
CTCAGCTTGGTCTTCTTTTATAGAAGAGGTTGAGGTGAAAGAGGTAAAAAATGCGCGTGTGAGTTTGACTGGGGAGAAAA
GTAGACCTGTAAAGCTTAAAGATGTTGATAATATCAATTACGCCCGAACTAAGACGGATATGGAGGAGTTTAACAGAGTT
CTTGGCGGAGGTGTCGTACCAGGCAGTTTAATCTTAATCGGTGGCGATCCGGGCATTGGAAAATCTACTTTGCTATTACA
AGTGTCCACACAATTAGCAAATAAGGGAACTGTCTTGTATGTATCTGGCGAAGAATCAGCCGAACAAATTAAGCTAAGAA
GTGAGCGTTTAGGTGATATTGATAATGAATTTTACCTTTATGCTGAAACCAATATGCAGAGCATTCGTTCGGAAATCGAA
AAAATAGCACCTGATTTTTTGATTATAGACTCTATTCAAACCATCATGAGTCCAGAGATTCAAGGTGTTCAGGGATCTGT
TAGTCAGGTCCGTGAAGTGACAGCTGAATTAATGCAATTGGCTAAAACGAATAATATAGCGACTTTCATTGTGGGTCATG
TCACAAAGGAAGGAACAATTGCTGGCCCACGGATGCTTGAACATATGGTTGACACTGTTCTTTATTTTGAAGGTGAAAGG
CATCATACCTTCCGAATTTTGAGGGCAGTTAAAAATCGATTCGGCTCTACAAATGAAATTGGTATTTTTGAAATGCAGTC
AGGTGGTCTGGTTGAAGTTCTCAATCCGAGTCAAGTGTTTTTAGAAGAACGCTTGGATGGTGCCACAGGTTCTGCCATTG
TCGTTACCATGGAAGGCAGTCGTCCGATTTTGGCTGAGGTCCAATCTTTAGTTACGCCTACCGTTTTTGGAAATGCCAGA
AGAACGACCACAGGTCTTGATTTTAACAGAATTAGCTTGATTATGGCTGTTTTAGAAAAACGATGTGGTCTTTTATTACA
AAATCAAGATGCCTATCTGAAGTCAGCTGGTGGAGTTAAGTTAGATGAACCTGCTATCGATTTGGCTGTCGCAGTAGCCA
TTGCCTCTAGCTACAAAGAAATGCCGACAAACCCCCAAGAAGCCTTTTTAGGTGAAATTGGGCTGACAGGTGAAATCAGA
AGGGTAACTCGAATGGAACAAAGAATCAACGAAGCGGCTAAATTAGGCTTTACAAAAATTTATGCCCCCAAAAATGCATT
ACATGGTATTGATATTCCCAAAGGGATTCAAGTTGTAGGGGTCACAACCGTCGGTGAGGTTTTAAAGGCAGTATTTTCCA
AAGCTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(55-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9DVX0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

87.417

99.56

0.87
  radA Streptococcus pneumoniae D39

87.417

99.56

0.87
  radA Streptococcus pneumoniae R6

87.417

99.56

0.87
  radA Streptococcus pneumoniae TIGR4

87.417

99.56

0.87
  radA Streptococcus mitis NCTC 12261

87.417

99.56

0.87
  radA Streptococcus mitis SK321

87.196

99.56

0.868
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

99.56

0.609

Multiple sequence alignment