Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQM82_RS01240 Genome accession   NZ_LS483386
Coordinates   218051..219412 (+) Length   453 a.a.
NCBI ID   WP_023612415.1    Uniprot ID   A0A5S4TFF9
Organism   Streptococcus pyogenes strain NCTC13742     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 213051..224412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM82_RS01225 (NCTC13742_00244) - 214745..216529 (+) 1785 WP_023612401.1 ABC transporter ATP-binding protein -
  DQM82_RS01230 (NCTC13742_00245) - 216647..217414 (+) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  DQM82_RS01235 (NCTC13742_00246) - 217524..217970 (+) 447 WP_002986111.1 dUTP diphosphatase -
  DQM82_RS01240 (NCTC13742_00247) radA 218051..219412 (+) 1362 WP_023612415.1 DNA repair protein RadA Machinery gene
  DQM82_RS01245 (NCTC13742_00248) - 219601..220098 (+) 498 WP_023612406.1 beta-class carbonic anhydrase -
  DQM82_RS01250 (NCTC13742_00249) - 220229..220939 (+) 711 WP_011017311.1 TIGR00266 family protein -
  DQM82_RS01255 (NCTC13742_00250) gltX 221121..222566 (+) 1446 WP_023612410.1 glutamate--tRNA ligase -
  DQM82_RS01260 (NCTC13742_00251) - 222961..224307 (+) 1347 WP_023610780.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49458.74 Da        Isoelectric Point: 5.8210

>NTDB_id=1139679 DQM82_RS01240 WP_023612415.1 218051..219412(+) (radA) [Streptococcus pyogenes strain NCTC13742]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGIQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQDIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1139679 DQM82_RS01240 WP_023612415.1 218051..219412(+) (radA) [Streptococcus pyogenes strain NCTC13742]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAATATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTATTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGATATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(56-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TFF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.651

100

0.857
  radA Streptococcus pneumoniae D39

85.43

100

0.854
  radA Streptococcus pneumoniae R6

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.43

100

0.854
  radA Streptococcus mitis SK321

85.43

100

0.854
  radA Streptococcus pneumoniae TIGR4

85.43

100

0.854
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment