Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQM55_RS01215 Genome accession   NZ_LS483364
Coordinates   219617..220984 (+) Length   455 a.a.
NCBI ID   WP_111675216.1    Uniprot ID   -
Organism   Streptococcus sanguinis strain NCTC11086     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 197393..222626 219617..220984 within 0


Gene organization within MGE regions


Location: 197393..222626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM55_RS01075 (NCTC11086_00210) - 197393..199099 (+) 1707 WP_111675194.1 ABC transporter ATP-binding protein -
  DQM55_RS11905 - 199101..199239 (+) 139 Protein_186 ABC transporter ATP-binding protein -
  DQM55_RS11910 - 199218..199946 (+) 729 Protein_187 ABC transporter ATP-binding protein -
  DQM55_RS11915 rcrQ 200082..200870 (+) 789 WP_415244968.1 ABC transporter ATP-binding protein Regulator
  DQM55_RS01085 (NCTC11086_00212) amiA 200987..202960 (-) 1974 WP_111675196.1 peptide ABC transporter substrate-binding protein Regulator
  DQM55_RS01090 (NCTC11086_00213) - 203189..203878 (+) 690 WP_002893948.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  DQM55_RS01100 (NCTC11086_00214) - 204144..204593 (+) 450 WP_002899180.1 8-oxo-dGTP diphosphatase -
  DQM55_RS11700 (NCTC11086_00215) - 205091..205435 (-) 345 WP_172454694.1 hypothetical protein -
  DQM55_RS01115 (NCTC11086_00216) - 205650..205841 (-) 192 WP_111675197.1 hypothetical protein -
  DQM55_RS01120 (NCTC11086_00217) - 206762..208027 (-) 1266 WP_111675198.1 phage portal protein -
  DQM55_RS01125 (NCTC11086_00218) - 208153..208482 (-) 330 WP_111675199.1 hypothetical protein -
  DQM55_RS01130 (NCTC11086_00219) - 208495..209040 (-) 546 WP_111675200.1 hypothetical protein -
  DQM55_RS01135 (NCTC11086_00220) - 209692..211341 (-) 1650 WP_111675201.1 phage/plasmid primase, P4 family -
  DQM55_RS01140 (NCTC11086_00221) - 211338..211853 (-) 516 WP_072074691.1 hypothetical protein -
  DQM55_RS01145 (NCTC11086_00222) - 211850..212140 (-) 291 WP_111675202.1 hypothetical protein -
  DQM55_RS01150 (NCTC11086_00223) - 212142..212360 (-) 219 WP_111675203.1 hypothetical protein -
  DQM55_RS01155 (NCTC11086_00224) - 212371..212613 (-) 243 WP_111675204.1 hypothetical protein -
  DQM55_RS01160 (NCTC11086_00225) - 212603..212899 (-) 297 WP_111675205.1 hypothetical protein -
  DQM55_RS11855 (NCTC11086_00226) - 212926..213060 (-) 135 WP_255297061.1 hypothetical protein -
  DQM55_RS01165 (NCTC11086_00227) - 213085..213345 (-) 261 WP_111675206.1 hypothetical protein -
  DQM55_RS01175 (NCTC11086_00229) - 213585..213992 (-) 408 WP_111675208.1 DUF1492 domain-containing protein -
  DQM55_RS01180 (NCTC11086_00230) - 214248..214433 (-) 186 WP_111675209.1 hypothetical protein -
  DQM55_RS01185 (NCTC11086_00231) - 214575..215213 (+) 639 WP_111675210.1 helix-turn-helix domain-containing protein -
  DQM55_RS01190 (NCTC11086_00232) - 215409..216386 (+) 978 WP_111675211.1 Abi family protein -
  DQM55_RS01195 (NCTC11086_00233) - 216724..217869 (+) 1146 WP_111675212.1 tyrosine-type recombinase/integrase -
  DQM55_RS01200 (NCTC11086_00234) - 217964..218410 (+) 447 WP_111675213.1 dUTP diphosphatase -
  DQM55_RS01205 (NCTC11086_00235) - 218412..218927 (+) 516 WP_111675214.1 histidine phosphatase family protein -
  DQM55_RS01210 (NCTC11086_00236) - 218979..219611 (+) 633 WP_111675215.1 methyltransferase domain-containing protein -
  DQM55_RS01215 (NCTC11086_00237) radA 219617..220984 (+) 1368 WP_111675216.1 DNA repair protein RadA Machinery gene
  DQM55_RS01220 (NCTC11086_00238) - 221101..221817 (+) 717 WP_111675217.1 TIGR00266 family protein -
  DQM55_RS01225 (NCTC11086_00239) - 221931..222626 (+) 696 WP_002893942.1 TIGR00266 family protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49804.17 Da        Isoelectric Point: 5.7873

>NTDB_id=1138647 DQM55_RS01215 WP_111675216.1 219617..220984(+) (radA) [Streptococcus sanguinis strain NCTC11086]
MTIAKKKTTFVCQNCEYHSPKYLGRCPNCGSWSSFVEEVEAVEVKHARVSLTGEKTRPMKLAEVTSIDVNRTKTEMDEFN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSHQGTVLYVSGEESAEQIKLRAERLGDIDSEFYLYAETNMQNIRTE
IEKIKPDFLIIDSIQTVMSPEISSVQGSVSQVREVTAELMQLAKTNNIATFIVGHMTKEGTLAGPRTLEHMVDTVLYFEG
ERQHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVINPSEVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGN
AKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDLPTNPQECFIGEIGLTGE
IRRVNRIEQRINEAAKLGFTKVYAPKNSLNGLKVPDNIQVIGVTTIGEVLKKVFS

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1138647 DQM55_RS01215 WP_111675216.1 219617..220984(+) (radA) [Streptococcus sanguinis strain NCTC11086]
ATCACCATCGCTAAGAAAAAAACGACCTTTGTCTGTCAAAATTGTGAATATCATTCGCCCAAGTATCTAGGTCGCTGTCC
TAACTGCGGCTCTTGGTCTTCTTTCGTTGAGGAAGTAGAGGCTGTAGAAGTCAAGCATGCTCGTGTTTCCTTGACAGGTG
AAAAGACCCGGCCCATGAAGCTGGCTGAGGTCACTTCGATTGATGTCAACCGAACCAAGACAGAAATGGATGAGTTCAAC
CGTGTGCTGGGTGGTGGCGTAGTGCCTGGCAGTTTGGTCCTGATTGGCGGGGATCCCGGTATCGGGAAGTCCACCTTGCT
TCTACAGGTATCCACTCAACTTTCTCATCAGGGCACCGTCCTTTATGTCAGCGGGGAGGAATCGGCTGAACAGATTAAGC
TGCGGGCGGAGCGACTCGGTGATATTGACAGTGAGTTTTATCTCTATGCCGAGACCAATATGCAAAATATCCGCACGGAG
ATTGAGAAAATCAAGCCAGATTTTCTGATTATTGACTCCATTCAGACAGTGATGTCGCCGGAAATTTCCAGTGTTCAAGG
TTCTGTTTCTCAGGTTCGAGAGGTGACAGCTGAGCTCATGCAGCTGGCCAAGACCAATAATATTGCGACCTTTATCGTTG
GTCACATGACCAAGGAAGGAACCTTAGCTGGCCCACGAACTTTGGAGCATATGGTGGACACCGTGCTTTATTTTGAGGGC
GAACGTCAGCACACTTTCCGTATTTTACGGGCAGTTAAAAACCGCTTTGGCTCTACCAATGAGATTGGTATTTTTGAGAT
GCAGTCTGGTGGGCTAGTTGAGGTTATCAACCCCAGTGAAGTCTTTCTGGAAGAGCGTCTGGATGGCGCGACAGGTTCTT
CTATCGTTGTGACCATGGAAGGAACGAGGCCGATTTTAGCTGAGGTGCAGGCTTTGGTGACACCGACCATGTTCGGCAAT
GCCAAGCGAACCACGACTGGACTGGACTTTAACCGAGCCAGCCTTATCATGGCGGTTCTGGAAAAACGGGCAGGGCTCCT
CCTGCAAAATCAAGACGCCTACCTCAAATCAGCTGGTGGTGTCAAGCTAGATGAGCCAGCCATTGATTTGGCAGTAGCGG
TCGCCATTGCTTCCAGCTACAAGGATCTGCCTACCAATCCTCAAGAGTGCTTTATCGGTGAAATTGGCCTGACTGGTGAA
ATCCGTCGAGTCAATCGCATCGAGCAGCGCATCAACGAAGCAGCAAAGCTGGGTTTCACCAAAGTCTACGCTCCTAAAAA
CTCTCTGAACGGCCTCAAAGTTCCAGACAATATCCAAGTCATTGGTGTGACGACGATTGGGGAAGTCTTAAAGAAAGTGT
TTTCCTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(63-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

Subunit ChlI of Mg-chelatase (ChlI)

(362-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

91.391

99.56

0.91
  radA Streptococcus pneumoniae Rx1

91.17

99.56

0.908
  radA Streptococcus pneumoniae D39

91.17

99.56

0.908
  radA Streptococcus pneumoniae R6

91.17

99.56

0.908
  radA Streptococcus pneumoniae TIGR4

91.17

99.56

0.908
  radA Streptococcus mitis SK321

91.17

99.56

0.908
  radA Bacillus subtilis subsp. subtilis str. 168

62.472

99.56

0.622

Multiple sequence alignment