Detailed information NTG1138317

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: DQM46_RS01435
• Genome accession: NZ_LS483357
• Coordinates: 262252..263322 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain NCTC8326
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 257252..268322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM46_RS01420 (NCTC8326_00286)	amiA	257771..259750 (+)	1980	WP_021299351.1	peptide ABC transporter substrate-binding protein	Regulator
DQM46_RS01425 (NCTC8326_00287)	amiC	259815..261317 (+)	1503	WP_011017334.1	ABC transporter permease	Regulator
DQM46_RS01430 (NCTC8326_00288)	amiD	261317..262243 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
DQM46_RS01435 (NCTC8326_00289)	amiE	262252..263322 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
DQM46_RS01440 (NCTC8326_00290)	amiF	263315..264238 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
DQM46_RS10115	-	264276..264359 (-)	84	Protein_230	IS3 family transposase	-
DQM46_RS10120	-	264493..264812 (-)	320	Protein_231	IS3 family transposase	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=1138317 DQM46_RS01435 WP_010921893.1 262252..263322(+) (amiE) [Streptococcus pyogenes strain NCTC8326]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=1138317 DQM46_RS01435 WP_010921893.1 262252..263322(+) (amiE) [Streptococcus pyogenes strain NCTC8326]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATTGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537