Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQL02_RS01195 Genome accession   NZ_LS483355
Coordinates   209592..210953 (+) Length   453 a.a.
NCBI ID   WP_002986109.1    Uniprot ID   A0A4Q1R665
Organism   Streptococcus pyogenes strain NCTC12067     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 177070..209511 209592..210953 flank 81


Gene organization within MGE regions


Location: 177070..210953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL02_RS01030 (NCTC12067_00204) polA 177070..179712 (+) 2643 WP_031488641.1 DNA polymerase I -
  DQL02_RS01035 (NCTC12067_00205) - 179890..180345 (+) 456 WP_111703048.1 CoA-binding protein -
  DQL02_RS01040 (NCTC12067_00206) perR 180397..180864 (+) 468 WP_002986328.1 peroxide-responsive transcriptional repressor PerR -
  DQL02_RS01045 - 181083..181319 (+) 237 WP_014635252.1 hypothetical protein -
  DQL02_RS01050 (NCTC12067_00208) - 181541..182875 (+) 1335 WP_031488638.1 phosphoadenosine phosphosulfate reductase -
  DQL02_RS01055 (NCTC12067_00209) - 182868..183398 (+) 531 WP_002987925.1 IbrB-like domain-containing protein -
  DQL02_RS08860 - 183445..183594 (-) 150 Protein_158 ISL3 family transposase -
  DQL02_RS01065 - 183726..184877 (-) 1152 Protein_159 IS3 family transposase -
  DQL02_RS01075 (NCTC12067_00214) - 184955..186265 (-) 1311 WP_031488784.1 SLC13 family permease -
  DQL02_RS01080 (NCTC12067_00215) nadC 186489..187361 (+) 873 WP_031488783.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DQL02_RS01090 (NCTC12067_00218) - 189089..189952 (-) 864 WP_031488685.1 DUF975 family protein -
  DQL02_RS09135 - 189989..190174 (+) 186 WP_002986321.1 hypothetical protein -
  DQL02_RS01100 (NCTC12067_00219) tgt 190171..191313 (+) 1143 WP_031488684.1 tRNA guanosine(34) transglycosylase Tgt -
  DQL02_RS01110 (NCTC12067_00220) - 191531..191842 (+) 312 WP_002987947.1 CHY zinc finger protein -
  DQL02_RS01115 (NCTC12067_00221) - 191846..192385 (+) 540 WP_002986314.1 biotin transporter BioY -
  DQL02_RS01120 (NCTC12067_00222) - 192525..193304 (+) 780 WP_011528209.1 MBL fold metallo-hydrolase -
  DQL02_RS01125 (NCTC12067_00223) tadA 193304..193819 (+) 516 WP_002992549.1 tRNA adenosine(34) deaminase TadA -
  DQL02_RS01130 (NCTC12067_00224) - 194433..195665 (-) 1233 WP_031488683.1 transglutaminase domain-containing protein -
  DQL02_RS01140 (NCTC12067_00226) speG 196065..196769 (+) 705 WP_031488682.1 streptococcal pyrogenic exotoxin SpeG -
  DQL02_RS01145 (NCTC12067_00228) - 197225..198574 (+) 1350 WP_014635263.1 glucose-6-phosphate isomerase -
  DQL02_RS01150 - 198923..200431 (-) 1509 WP_002986134.1 helix-turn-helix domain-containing protein -
  DQL02_RS01155 (NCTC12067_00231) - 201119..201790 (+) 672 WP_031488681.1 rhomboid family intramembrane serine protease -
  DQL02_RS01160 (NCTC12067_00232) galU 201889..202788 (-) 900 WP_002986125.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  DQL02_RS01165 (NCTC12067_00233) - 202821..203837 (-) 1017 WP_002986123.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  DQL02_RS01170 (NCTC12067_00234) - 204135..204584 (+) 450 WP_002986120.1 MarR family winged helix-turn-helix transcriptional regulator -
  DQL02_RS01175 (NCTC12067_00235) - 204577..206283 (+) 1707 WP_031488680.1 ABC transporter ATP-binding protein -
  DQL02_RS01180 (NCTC12067_00236) - 206286..208070 (+) 1785 WP_023610145.1 ABC transporter ATP-binding protein -
  DQL02_RS01185 (NCTC12067_00237) - 208188..208955 (+) 768 WP_011054158.1 epoxyqueuosine reductase QueH -
  DQL02_RS01190 (NCTC12067_00238) - 209065..209511 (+) 447 WP_002986111.1 dUTP diphosphatase -
  DQL02_RS01195 (NCTC12067_00239) radA 209592..210953 (+) 1362 WP_002986109.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49386.68 Da        Isoelectric Point: 6.0962

>NTDB_id=1138203 DQL02_RS01195 WP_002986109.1 209592..210953(+) (radA) [Streptococcus pyogenes strain NCTC12067]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1138203 DQL02_RS01195 WP_002986109.1 209592..210953(+) (radA) [Streptococcus pyogenes strain NCTC12067]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAATATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCGGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATCGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q1R665

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.872

100

0.859
  radA Streptococcus pneumoniae D39

85.651

100

0.857
  radA Streptococcus pneumoniae R6

85.651

100

0.857
  radA Streptococcus pneumoniae Rx1

85.651

100

0.857
  radA Streptococcus mitis SK321

85.651

100

0.857
  radA Streptococcus pneumoniae TIGR4

85.651

100

0.857
  radA Bacillus subtilis subsp. subtilis str. 168

61.369

100

0.614

Multiple sequence alignment