Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OB04_RS13150 Genome accession   NZ_CP009796
Coordinates   2530872..2531282 (-) Length   136 a.a.
NCBI ID   WP_038429332.1    Uniprot ID   -
Organism   Bacillus subtilis strain SG6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2525872..2536282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OB04_RS13110 - 2525958..2526098 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  OB04_RS13120 (OB04_02571) yqeF 2526404..2527135 (-) 732 WP_015714294.1 SGNH/GDSL hydrolase family protein -
  OB04_RS13130 (OB04_02572) cwlH 2527388..2528140 (-) 753 WP_038429329.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  OB04_RS13135 (OB04_02573) yqeD 2528327..2528953 (+) 627 WP_038429330.1 TVP38/TMEM64 family protein -
  OB04_RS13140 (OB04_02574) gnd 2528972..2529865 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  OB04_RS13145 (OB04_02575) yqeB 2530117..2530839 (+) 723 WP_010886572.1 hypothetical protein -
  OB04_RS13150 (OB04_02576) nucA/comI 2530872..2531282 (-) 411 WP_038429332.1 sporulation-specific Dnase NucB Machinery gene
  OB04_RS13155 (OB04_02577) sigK 2531478..2532206 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  OB04_RS22445 - 2532206..2532298 (+) 93 Protein_2556 hypothetical protein -
  OB04_RS21735 (OB04_02578) - 2532281..2532421 (-) 141 Protein_2557 recombinase -
  OB04_RS13170 (OB04_02579) yrkL 2532966..2533490 (-) 525 WP_038429334.1 NAD(P)H-dependent oxidoreductase -
  OB04_RS13175 (OB04_02580) yrkK 2533738..2534212 (-) 475 Protein_2559 HXXEE domain-containing protein -
  OB04_RS13180 (OB04_02582) - 2534716..2536095 (-) 1380 WP_038429335.1 aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14891.88 Da        Isoelectric Point: 5.1854

>NTDB_id=113759 OB04_RS13150 WP_038429332.1 2530872..2531282(-) (nucA/comI) [Bacillus subtilis strain SG6]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSSPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=113759 OB04_RS13150 WP_038429332.1 2530872..2531282(-) (nucA/comI) [Bacillus subtilis strain SG6]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTCCCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.739

84.559

0.522