Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQL28_RS01165 Genome accession   NZ_LS483334
Coordinates   201066..202427 (+) Length   453 a.a.
NCBI ID   WP_002986109.1    Uniprot ID   A0A4Q1R665
Organism   Streptococcus pyogenes strain NCTC12050     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 196066..207427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL28_RS01150 (NCTC12050_00223) - 197760..199544 (+) 1785 WP_111676101.1 ABC transporter ATP-binding protein -
  DQL28_RS01155 (NCTC12050_00224) - 199662..200429 (+) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  DQL28_RS01160 (NCTC12050_00226) - 200539..200985 (+) 447 WP_002986111.1 dUTP diphosphatase -
  DQL28_RS01165 (NCTC12050_00227) radA 201066..202427 (+) 1362 WP_002986109.1 DNA repair protein RadA Machinery gene
  DQL28_RS01170 (NCTC12050_00228) - 202616..203113 (+) 498 WP_111676103.1 beta-class carbonic anhydrase -
  DQL28_RS01175 (NCTC12050_00229) - 203244..203954 (+) 711 WP_168389576.1 TIGR00266 family protein -
  DQL28_RS01180 (NCTC12050_00230) gltX 204136..205581 (+) 1446 WP_002986105.1 glutamate--tRNA ligase -
  DQL28_RS01185 (NCTC12050_00231) - 205976..207322 (+) 1347 WP_111676105.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49386.68 Da        Isoelectric Point: 6.0962

>NTDB_id=1137063 DQL28_RS01165 WP_002986109.1 201066..202427(+) (radA) [Streptococcus pyogenes strain NCTC12050]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1137063 DQL28_RS01165 WP_002986109.1 201066..202427(+) (radA) [Streptococcus pyogenes strain NCTC12050]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAATATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGGCCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTTTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q1R665

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.872

100

0.859
  radA Streptococcus pneumoniae D39

85.651

100

0.857
  radA Streptococcus pneumoniae R6

85.651

100

0.857
  radA Streptococcus pneumoniae Rx1

85.651

100

0.857
  radA Streptococcus mitis SK321

85.651

100

0.857
  radA Streptococcus pneumoniae TIGR4

85.651

100

0.857
  radA Bacillus subtilis subsp. subtilis str. 168

61.369

100

0.614

Multiple sequence alignment