Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQL27_RS01305 Genome accession   NZ_LS483329
Coordinates   222555..223916 (+) Length   453 a.a.
NCBI ID   WP_076634182.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC12058     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 217555..228916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL27_RS01290 (NCTC12058_00256) - 219249..221033 (+) 1785 WP_002992923.1 ABC transporter ATP-binding protein -
  DQL27_RS01295 (NCTC12058_00257) - 221151..221918 (+) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  DQL27_RS01300 (NCTC12058_00258) - 222028..222474 (+) 447 WP_002986111.1 dUTP diphosphatase -
  DQL27_RS01305 (NCTC12058_00259) radA 222555..223916 (+) 1362 WP_076634182.1 DNA repair protein RadA Machinery gene
  DQL27_RS01310 (NCTC12058_00260) - 224105..224602 (+) 498 WP_076634181.1 beta-class carbonic anhydrase -
  DQL27_RS01315 (NCTC12058_00261) - 224733..225443 (+) 711 WP_002991188.1 TIGR00266 family protein -
  DQL27_RS01320 (NCTC12058_00262) gltX 225625..227070 (+) 1446 WP_076634180.1 glutamate--tRNA ligase -
  DQL27_RS01325 (NCTC12058_00263) - 227465..228811 (+) 1347 WP_002991186.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49399.76 Da        Isoelectric Point: 6.6511

>NTDB_id=1136786 DQL27_RS01305 WP_076634182.1 222555..223916(+) (radA) [Streptococcus pyogenes strain NCTC12058]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIKVVGVTTIGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1136786 DQL27_RS01305 WP_076634182.1 222555..223916(+) (radA) [Streptococcus pyogenes strain NCTC12058]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCGGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATCGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTAAGGTTGTCGGAGTGACTACGATTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

86.093

100

0.861
  radA Streptococcus pneumoniae D39

85.872

100

0.859
  radA Streptococcus pneumoniae R6

85.872

100

0.859
  radA Streptococcus pneumoniae Rx1

85.872

100

0.859
  radA Streptococcus mitis SK321

85.872

100

0.859
  radA Streptococcus pneumoniae TIGR4

85.872

100

0.859
  radA Bacillus subtilis subsp. subtilis str. 168

60.927

100

0.609

Multiple sequence alignment