Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQL35_RS01260 Genome accession   NZ_LS483325
Coordinates   241786..243147 (+) Length   453 a.a.
NCBI ID   WP_012514884.1    Uniprot ID   B4U0R5
Organism   Streptococcus equi subsp. zooepidemicus strain NCTC7022     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 236786..248147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL35_RS01245 (NCTC7022_00251) - 238347..240143 (+) 1797 WP_111677698.1 ABC transporter ATP-binding protein -
  DQL35_RS01250 (NCTC7022_00252) - 240305..241072 (+) 768 WP_043040009.1 epoxyqueuosine reductase QueH -
  DQL35_RS01255 (NCTC7022_00253) - 241273..241719 (+) 447 WP_111677699.1 dUTP diphosphatase -
  DQL35_RS01260 (NCTC7022_00254) radA 241786..243147 (+) 1362 WP_012514884.1 DNA repair protein RadA Machinery gene
  DQL35_RS01265 (NCTC7022_00255) - 243344..243586 (-) 243 WP_111677700.1 hypothetical protein -
  DQL35_RS01270 (NCTC7022_00256) - 243918..244424 (+) 507 WP_111677701.1 beta-class carbonic anhydrase -
  DQL35_RS01275 (NCTC7022_00257) - 244552..245265 (+) 714 WP_111677702.1 TIGR00266 family protein -
  DQL35_RS01280 (NCTC7022_00258) gltX 245867..247312 (+) 1446 WP_111677703.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49333.77 Da        Isoelectric Point: 6.7545

>NTDB_id=1136557 DQL35_RS01260 WP_012514884.1 241786..243147(+) (radA) [Streptococcus equi subsp. zooepidemicus strain NCTC7022]
MAKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLKHVNGVRYQRIQTEMNEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLYVSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIE
AIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQALVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKLYAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1136557 DQL35_RS01260 WP_012514884.1 241786..243147(+) (radA) [Streptococcus equi subsp. zooepidemicus strain NCTC7022]
ATAGCTAAGAAAAAGGCGAGGTTTATCTGTGGAGAATGTGGCTATCAGTCACCCAAATATCTGGGGCGCTGCCCTAATTG
CTCTGCCTGGTCTTCCTTTGTAGAGGAGACAGAGGTCAAGGACGTCAAAAATGCCCGTATTAGCCTGACTGGAGAAAAAA
GTAAGCCCATCAAGCTAAAGCATGTCAATGGCGTTCGCTACCAGCGTATCCAGACAGAGATGAATGAGTTCAATCGTGTG
CTGGGCGGTGGTGTTGTGCCGGGGAGCTTGGTTTTGATTGGTGGTGATCCTGGTATTGGTAAGTCAACGCTACTCTTGCA
AGTTTCTATCCAGCTTGCGGATAAGGGAACTGTTCTATATGTCTCTGGAGAGGAATCGGCAGAGCAGATCAAGATGCGCA
GTGATCGCTTGGGACAGACCGACAATGACTTTTACCTCTATGCGGAGACCAATATGCAGGCTATTCGGGCAGAGATTGAA
GCCATTCAGCCGGATTTTTTGATTATTGATTCTATTCAGACGATCATGAGTCCGGAGATTTCGGGTGTTCAGGGCTCGGT
CAGTCAGGTGAGAGAGGTTACTGCTGAGCTACTCCAGCTAGCTAAGTCTAATCATATTGCAACCTTTATCGTAGGACATG
TCACCAAGGAGGGTACCCTAGCAGGGCCACGTATGCTGGAGCATATGGTTGATACTGTGCTTTATTTTGAAGGGGAAAGA
CACCACACCTTCCGAATTCTGCGAGCTGTTAAAAACCGTTTTGGCTCAACCAATGAGATTGGTATTTTTGACATGCAATC
AGGTGGCTTAGTGGAGGTTCTCAATCCCAGTCAGGTTTTCTTAGAGGAACGATTAGATGGGGCAACAGGCTCTGCTGTGG
TTGTCACCATGGAGGGGAGCAGACCGATTTTGGCAGAGGTGCAGGCCTTAGTCACACCGACTGTTTTTGGCAATGCTAGA
CGAACCACTACTGGATTAGACTTTAATCGTGTTAGCCTGATTATGGCTGTCCTAGAAAAGCGCTGCGGCCTGCTGCTACA
AAATCAAGATGCCTACCTCAAATCAGCAGGAGGTGTGAAGCTAGATGAGCCTGCTATTGATTTGGCAGTTGCTGTGGCTA
TTGCTTCTAGCTACAAGGAAAAACCAACCAATCCTCAGGAGGCCTTCTTAGGAGAGATTGGCTTGACGGGCGAGATTCGG
CGGGTCACTCGTATTGAGCAGCGGATCAATGAGGCTGCAAAGCTTGGCTTTACCAAGCTTTACGCTCCTAAAAGCTCATT
GCAGGGCATTGACATACCAAGTGGGATTGAGGTTGTTGGTGTAATGACAGTAGGAGAGGTCTTAAAGGCAGTCTTTGCTT
AG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4U0R5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

84.327

100

0.843
  radA Streptococcus pneumoniae D39

84.327

100

0.843
  radA Streptococcus pneumoniae R6

84.327

100

0.843
  radA Streptococcus pneumoniae TIGR4

84.327

100

0.843
  radA Streptococcus mitis SK321

84.327

100

0.843
  radA Streptococcus mitis NCTC 12261

84.106

100

0.841
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment