Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   DQL82_RS00220 Genome accession   NZ_LS483319
Coordinates   45946..47574 (-) Length   542 a.a.
NCBI ID   WP_016170668.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NCTC13140     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39202..48932 45946..47574 within 0


Gene organization within MGE regions


Location: 39202..48932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL82_RS00180 (NCTC13140_00035) mecA 39202..41211 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  DQL82_RS00185 (NCTC13140_00036) - 41308..42294 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  DQL82_RS00190 (NCTC13140_00037) - 42284..42526 (+) 243 Protein_32 type I restriction enzyme endonuclease domain-containing protein -
  DQL82_RS00195 (NCTC13140_00038) - 42517..44127 (-) 1611 Protein_33 IS1182 family transposase -
  DQL82_RS00200 (NCTC13140_00039) - 44176..44682 (-) 507 WP_001092188.1 DUF1643 domain-containing protein -
  DQL82_RS00205 (NCTC13140_00040) - 44697..45008 (-) 312 WP_000700851.1 DUF960 family protein -
  DQL82_RS00215 (NCTC13140_00042) - 45095..45445 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  DQL82_RS00220 (NCTC13140_00043) ccrB 45946..47574 (-) 1629 WP_016170668.1 cassette chromosome recombinase CcrB Machinery gene
  DQL82_RS00225 (NCTC13140_00044) ccrA 47571..48932 (-) 1362 WP_016170667.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62405.78 Da        Isoelectric Point: 9.7442

>NTDB_id=1136238 DQL82_RS00220 WP_016170668.1 45946..47574(-) (ccrB) [Staphylococcus aureus strain NCTC13140]
MKQLKKKRIGLYARVSTEMQTDGYSIQGQLNQLTEYCQFQGYEVVDEYTDRGISGKTMQRPELQRMLKDANEGKLDCIMV
YKTNRLARNTSDLLNIVEELYKINVEFFSLTEKIEIASSTGKLMLQILASFAEFERNTIVENVYNGQRQRAIEGYYQGNL
PLGYDKVPDSKKELMINQHEANIVKYIFKSYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYIISNPFYIGKIQFAKY
RHWSDKRRKGLNEEPIIADGKHAPIIDKALWDKVQFKRQESRKKPQVHGKGTNLLTGIVKCPKCGAAMAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRADVLEKYVMDQILEIIKSKKVLKQLVEKVNKKSQIDVSSLNHDIAYKQSQCEE
IKAKMHTLTKTIEDSPDLDSVLKPTILSYQDELNQINNQIHQLEQDKQAEAPHYDADMIANVLQAIFKDIDKLEKSQLKS
LYLTVIDRIDIRKHEHHKKQFYVTLKLNNDIIKQLFDNHPLDEVLLSTSSLFLSQTLYITIE

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=1136238 DQL82_RS00220 WP_016170668.1 45946..47574(-) (ccrB) [Staphylococcus aureus strain NCTC13140]
ATGAAACAACTCAAAAAGAAACGTATTGGATTATACGCACGTGTATCAACTGAAATGCAAACGGATGGTTACAGTATTCA
AGGTCAATTAAACCAACTTACAGAGTATTGTCAATTTCAAGGATATGAAGTCGTTGATGAATATACCGATCGAGGTATAT
CGGGTAAAACAATGCAACGTCCAGAATTACAACGTATGTTAAAAGATGCCAACGAAGGTAAATTAGATTGTATTATGGTC
TACAAAACCAATCGTTTAGCTAGAAATACCTCAGATTTGTTAAATATTGTCGAGGAATTGTATAAGATTAATGTCGAATT
TTTTAGTCTCACTGAAAAAATAGAAATTGCCAGCTCCACAGGTAAGTTGATGCTCCAAATTTTAGCCAGCTTCGCAGAAT
TTGAAAGAAATACGATTGTAGAGAATGTCTACAATGGCCAAAGACAACGTGCGATTGAAGGTTATTATCAAGGTAATTTG
CCTCTAGGCTATGATAAAGTACCTGATAGTAAAAAAGAGCTCATGATTAATCAACATGAAGCGAATATTGTTAAATATAT
CTTTAAGTCATACGCCAAAGGTCATGGTTATCGTAAGATTGCGAACGCATTGAATCATAAAGGTTATGTGACTAAGAAAG
GGAAACCGTTTAGTATTTCATCCATTACTTATATCATCTCAAACCCTTTTTATATTGGAAAGATACAGTTTGCGAAGTAT
AGACACTGGAGCGATAAGAGACGAAAAGGACTCAATGAGGAACCCATTATTGCTGATGGTAAGCATGCACCTATTATTGA
TAAAGCTTTATGGGATAAAGTACAGTTTAAACGCCAAGAGAGTCGCAAGAAACCTCAAGTTCATGGTAAAGGGACCAATC
TACTGACTGGAATTGTAAAGTGCCCAAAATGTGGTGCAGCCATGGCGGCAAGTAATACAACCAATACGTTAAAAGATGGC
ACTAAGAAAAGAATCAGATATTACTCATGTAGTAATTTTAGAAACAAAGGTTCTAAAGTATGTTCAGCCAATAGTGTTAG
AGCAGATGTATTAGAAAAATATGTTATGGATCAGATATTGGAAATTATCAAAAGTAAAAAGGTATTGAAACAATTGGTTG
AAAAGGTAAATAAAAAAAGTCAAATAGACGTCTCTTCTTTGAATCATGATATTGCCTATAAACAGTCACAATGTGAAGAA
ATAAAAGCCAAAATGCATACACTAACGAAAACCATCGAAGACTCACCTGACTTAGATAGCGTATTGAAACCAACAATACT
TAGCTACCAAGATGAATTGAATCAAATAAATAATCAAATACATCAACTAGAACAAGATAAACAAGCAGAAGCTCCACATT
ATGATGCTGATATGATTGCCAACGTACTACAAGCAATATTCAAAGATATAGACAAATTAGAGAAATCTCAGTTGAAATCA
CTCTATCTGACAGTTATCGATCGTATTGATATCCGAAAACATGAACATCATAAGAAGCAATTTTATGTTACGCTCAAATT
GAATAACGATATTATTAAACAACTATTTGATAATCATCCACTTGACGAAGTGCTCCTTAGCACTTCGTCTTTATTTTTGT
CCCAAACTTTATATATAACTATTGAATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-154)

Ogr/Delta-like zinc finger (Ogr_Delta)

(300-355)

Recombinase (Recombinase)

(179-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

72.694

100

0.727

Multiple sequence alignment