Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   DQL89_RS00375 Genome accession   NZ_LS483313
Coordinates   81390..82751 (-) Length   453 a.a.
NCBI ID   WP_103364426.1    Uniprot ID   -
Organism   Staphylococcus simulans strain NCTC11046     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 54774..87173 81390..82751 within 0


Gene organization within MGE regions


Location: 54774..87173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL89_RS00245 (NCTC11046_00049) ccrC 54774..56459 (+) 1686 WP_032603677.1 cassette chromosome recombinase CcrC -
  DQL89_RS00250 (NCTC11046_00050) - 56548..56886 (+) 339 WP_002504276.1 SAUGI family uracil-DNA glycosylase inhibitor -
  DQL89_RS00260 (NCTC11046_00052) - 56980..57291 (+) 312 WP_002449385.1 DUF960 family protein -
  DQL89_RS00265 (NCTC11046_00053) - 57307..57810 (+) 504 WP_002504277.1 DUF1643 domain-containing protein -
  DQL89_RS00270 (NCTC11046_00054) - 57825..58046 (+) 222 WP_002504278.1 hypothetical protein -
  DQL89_RS00275 (NCTC11046_00056) - 58306..58713 (+) 408 WP_002504279.1 DUF2992 family protein -
  DQL89_RS00280 (NCTC11046_00057) - 58766..61885 (-) 3120 WP_103364226.1 type I restriction endonuclease subunit R -
  DQL89_RS00285 (NCTC11046_00058) - 61869..63164 (-) 1296 WP_002504281.1 restriction endonuclease subunit S -
  DQL89_RS00290 (NCTC11046_00059) - 63154..64668 (-) 1515 WP_002504282.1 type I restriction-modification system subunit M -
  DQL89_RS00295 (NCTC11046_00060) - 64801..65364 (+) 564 WP_231911565.1 hypothetical protein -
  DQL89_RS00300 (NCTC11046_00061) - 65431..65928 (+) 498 WP_231911591.1 HEPN domain-containing protein -
  DQL89_RS00305 (NCTC11046_00062) - 66560..67234 (+) 675 WP_064587222.1 IS6 family transposase -
  DQL89_RS00310 (NCTC11046_00063) - 67371..68444 (+) 1074 WP_002504431.1 hypothetical protein -
  DQL89_RS00315 (NCTC11046_00064) - 68479..69228 (+) 750 WP_002504430.1 hypothetical protein -
  DQL89_RS00320 (NCTC11046_00065) - 69270..70247 (+) 978 WP_002504429.1 DUF1002 domain-containing protein -
  DQL89_RS00325 (NCTC11046_00066) - 70432..73224 (+) 2793 WP_103364435.1 type I restriction endonuclease subunit R -
  DQL89_RS00330 (NCTC11046_00067) - 73315..73896 (+) 582 WP_103364434.1 restriction endonuclease subunit S -
  DQL89_RS00335 (NCTC11046_00068) - 73897..75453 (+) 1557 WP_103364433.1 type I restriction-modification system subunit M -
  DQL89_RS00340 (NCTC11046_00069) - 75446..76675 (+) 1230 WP_103364432.1 restriction endonuclease subunit S -
  DQL89_RS00345 (NCTC11046_00070) - 76986..77483 (-) 498 WP_103364431.1 GNAT family N-acetyltransferase -
  DQL89_RS00350 (NCTC11046_00071) - 77761..77982 (-) 222 WP_103364430.1 hypothetical protein -
  DQL89_RS00355 (NCTC11046_00072) - 77996..78499 (-) 504 WP_162496177.1 DUF1643 domain-containing protein -
  DQL89_RS00360 (NCTC11046_00073) - 78514..78825 (-) 312 WP_103364428.1 DUF960 domain-containing protein -
  DQL89_RS00365 (NCTC11046_00075) - 78912..79262 (-) 351 WP_103364427.1 SAUGI family uracil-DNA glycosylase inhibitor -
  DQL89_RS00370 (NCTC11046_00076) ccrB 79765..81393 (-) 1629 WP_001631035.1 cassette chromosome recombinase CcrB Machinery gene
  DQL89_RS00375 (NCTC11046_00077) ccrA 81390..82751 (-) 1362 WP_103364426.1 cassette chromosome recombinase CcrA Machinery gene
  DQL89_RS00380 (NCTC11046_00078) - 82938..83567 (-) 630 Protein_72 hypothetical protein -
  DQL89_RS00385 - 83576..83914 (+) 339 Protein_73 recombinase family protein -
  DQL89_RS00390 (NCTC11046_00080) - 84026..84385 (-) 360 WP_001631039.1 hypothetical protein -
  DQL89_RS00395 (NCTC11046_00081) - 84592..84918 (+) 327 WP_000391495.1 ArsR/SmtB family transcription factor -
  DQL89_RS00405 (NCTC11046_00083) - 85248..87173 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53253.38 Da        Isoelectric Point: 9.9020

>NTDB_id=1135967 DQL89_RS00375 WP_103364426.1 81390..82751(-) (ccrA) [Staphylococcus simulans strain NCTC11046]
MVEAIGYVRQSTLKQQSLATQQSLILNTAKQYGWSNVAFYDDKKTGRHTKRSGYQKMVEIITSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKKHHITIISVNDGYFDLSKEFDCFRLNILMSLAEMESNNISEQTKNGIREKAKQGKLITTHAPFGYRYR
KGCFVVQEEEAYTVKSVYRWYLQGLGYKKISQHLDSNPNLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIIDIDT
FEEAQERRIHKQLNRSNSRNKLKRRIKCPYCQSTLTNLTIKKENYSLRYYVCPKNMNDAYRTCPFKSINAPELESEVLDT
CKKYFEEQSFHKRLNNTILKVLKQQQMKHKETHLTQEQLIEKLAQNRIDVKTFKRLSASCKSEENYSTYSTYQIDDKLKY
VIKNKITLQDIAPFIDCIKITQTKQLQGIYFKNSPLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1135967 DQL89_RS00375 WP_103364426.1 81390..82751(-) (ccrA) [Staphylococcus simulans strain NCTC11046]
ATGGTAGAAGCCATTGGTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAGCAATCTCTGATTTTAAA
TACTGCTAAGCAATATGGATGGTCTAATGTGGCGTTTTATGATGATAAGAAAACAGGTCGACATACAAAACGTTCAGGTT
ATCAAAAAATGGTAGAAATAATTACGTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCTTGAACCGACTACATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAAAGCATCACATCACAATCATCAGTGTTAACGATGGATATTTTGA
TTTGTCTAAAGAGTTTGATTGCTTTCGTTTGAACATACTTATGAGTCTAGCGGAAATGGAATCAAACAATATCAGCGAAC
AAACAAAAAATGGGATTAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGCTATCGTTATAGA
AAAGGTTGTTTTGTTGTACAAGAAGAAGAAGCGTATACAGTTAAATCGGTTTATCGCTGGTACTTACAAGGTCTAGGCTA
CAAAAAGATTTCCCAACATTTGGATAGTAATCCTAACCTTATTCCTCGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAACTATTGCGGTCGTGTCATCAATAAATATGGCACGTTTAATGATATCGTTCCACCTATTATTGATATTGATACA
TTTGAAGAAGCACAAGAGCGTCGTATTCATAAACAACTCAATCGATCCAATTCTAGAAATAAGCTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAGAACTATTCTCTACGTTACTATGTTTGTCCTA
AAAATATGAATGACGCTTATCGTACATGCCCATTTAAAAGTATCAATGCACCAGAACTTGAAAGTGAAGTGCTCGACACT
TGTAAAAAGTATTTTGAAGAACAATCGTTTCATAAACGATTGAATAACACAATACTCAAAGTCCTAAAACAGCAACAAAT
GAAGCATAAAGAAACCCATCTAACACAAGAACAACTTATCGAAAAGCTAGCTCAAAATCGTATTGATGTAAAAACGTTCA
AACGTTTATCTGCTAGTTGTAAAAGTGAAGAGAATTACTCTACTTATTCAACCTACCAAATCGATGACAAATTAAAATAT
GTCATAAAAAATAAAATAACTTTACAGGATATAGCACCTTTTATCGATTGCATCAAAATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAACAGTCCACTCAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTAATAT
GA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

54.628

97.792

0.534
  ccrA Staphylococcus aureus N315

50.564

97.792

0.494

Multiple sequence alignment