Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DQL78_RS00270 Genome accession   NZ_LS483306
Coordinates   56588..57958 (+) Length   456 a.a.
NCBI ID   WP_016250427.1    Uniprot ID   S1RPR1
Organism   Enterococcus cecorum strain NCTC12421     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 51588..62958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL78_RS00245 (NCTC12421_00051) - 52940..54187 (-) 1248 WP_016250431.1 glutamate-5-semialdehyde dehydrogenase -
  DQL78_RS00250 (NCTC12421_00052) proB 54203..55006 (-) 804 WP_235426847.1 glutamate 5-kinase -
  DQL78_RS00255 (NCTC12421_00053) - 55322..55831 (-) 510 WP_016250429.1 hypothetical protein -
  DQL78_RS00265 (NCTC12421_00054) - 56054..56575 (+) 522 WP_026209954.1 hypothetical protein -
  DQL78_RS00270 (NCTC12421_00055) radA 56588..57958 (+) 1371 WP_016250427.1 DNA repair protein RadA Machinery gene
  DQL78_RS00275 (NCTC12421_00056) - 58158..59264 (+) 1107 WP_016250426.1 PIN/TRAM domain-containing protein -
  DQL78_RS00280 (NCTC12421_00057) - 59304..59861 (+) 558 WP_016250425.1 dTDP-4-dehydrorhamnose 3,5-epimerase family protein -
  DQL78_RS00285 (NCTC12421_00058) - 60155..60424 (+) 270 WP_231482343.1 hypothetical protein -
  DQL78_RS12655 - 60493..60621 (+) 129 WP_018345333.1 hypothetical protein -
  DQL78_RS00290 (NCTC12421_00059) - 60646..60969 (+) 324 WP_016250423.1 GNAT family N-acetyltransferase -
  DQL78_RS00295 (NCTC12421_00060) gltX 61064..62521 (+) 1458 WP_016250422.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49914.75 Da        Isoelectric Point: 7.3668

>NTDB_id=1135707 DQL78_RS00270 WP_016250427.1 56588..57958(+) (radA) [Enterococcus cecorum strain NCTC12421]
MAKKAKTQFECQHCGYISPRYLGKCPNCNSWNSMMEVKIENTTDVRQAVSFTGIKSQPTKITEVVPKKTPCVQTEIGELN
RVLGGGVVPGSLILLGGDPGIGKSTLLLQVSEQLTKAHGKVLYVSGEESAEQIKMRAERLGVLHESFYVYAENDMEAIRQ
AIEQLSPDYVIIDSIQTMIQPSISSVAGSVSQVRETTAELMRIAKSNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GERQQSFRILRAVKNRFGSTNEIGIFEMHEDGLHEVINPSQVFLEERLQGATGSAIVVTMEGTRPLLVEIQALITPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGMLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQATDCFIGEIGLTG
EIRRVNAIEQRIKEVQKLGFKRVFVPKNNLANLEIPAGIEVIGVSTLAETMKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1135707 DQL78_RS00270 WP_016250427.1 56588..57958(+) (radA) [Enterococcus cecorum strain NCTC12421]
GTGGCAAAAAAAGCAAAAACACAATTTGAGTGTCAACATTGTGGCTATATTTCCCCACGTTATTTAGGGAAATGTCCGAA
TTGTAATAGTTGGAACTCGATGATGGAAGTCAAAATCGAAAATACCACCGATGTCCGTCAAGCTGTTTCGTTTACCGGAA
TCAAGAGCCAACCAACCAAAATTACCGAAGTGGTGCCGAAAAAAACACCGTGCGTCCAAACAGAAATTGGTGAGTTGAAT
CGCGTGCTAGGTGGCGGCGTGGTGCCAGGTTCCTTGATTTTACTAGGAGGCGATCCGGGTATTGGCAAGTCGACACTGTT
ATTACAAGTATCCGAGCAGCTAACCAAAGCCCACGGGAAAGTCTTGTACGTCTCAGGTGAAGAAAGTGCCGAGCAAATCA
AGATGCGTGCGGAACGTCTAGGGGTCTTGCATGAGTCCTTTTATGTCTATGCGGAAAATGATATGGAGGCCATTCGTCAA
GCCATCGAACAACTTTCGCCTGATTATGTGATTATTGATTCGATTCAAACCATGATTCAGCCAAGCATTTCTTCTGTGGC
GGGAAGTGTCAGTCAGGTTCGCGAAACCACCGCTGAGTTGATGCGCATTGCTAAGAGTAATCAGATTGCCATTTTTATCG
TTGGTCATGTGACAAAAGAAGGGGCACTAGCAGGTCCTAGAATGTTAGAACACATGGTTGATACCGTGTTATATTTTGAA
GGAGAACGCCAACAGTCCTTTAGAATTTTACGTGCGGTGAAAAATCGTTTCGGTTCGACCAATGAAATCGGCATTTTCGA
GATGCATGAAGATGGGTTGCATGAAGTCATCAATCCTTCGCAAGTCTTTTTAGAAGAACGTCTGCAAGGAGCGACTGGTT
CGGCAATCGTGGTGACGATGGAAGGAACGCGCCCATTATTAGTGGAGATTCAAGCGTTGATTACACCCACCATGTTTGGA
AATGCCAAGCGTACCACGACAGGTCTAGACTTTAATCGGGTATCTTTAATTATGGCGGTCTTGGAAAAGCGCGCTGGAAT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCAGCCGGTGGTGTGAAGCTAAACGAGCCAGCAATCGATTTAGCTATTG
CGGTGAGTATTGCCTCTAGTTACAAGGAAAAAGGCACCCAGGCAACGGATTGTTTTATCGGTGAAATTGGCTTGACCGGT
GAAATCAGACGCGTCAATGCCATCGAACAACGCATCAAAGAAGTGCAAAAGTTAGGCTTTAAACGGGTATTTGTACCTAA
AAATAATTTAGCTAATTTAGAAATTCCAGCGGGTATTGAAGTTATCGGGGTTTCTACTTTGGCTGAAACGATGAAAAAAG
TCTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(74-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S1RPR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

70.549

99.781

0.704
  radA Streptococcus pneumoniae D39

70.549

99.781

0.704
  radA Streptococcus pneumoniae R6

70.549

99.781

0.704
  radA Streptococcus pneumoniae TIGR4

70.549

99.781

0.704
  radA Streptococcus mitis SK321

70.549

99.781

0.704
  radA Streptococcus mitis NCTC 12261

70.549

99.781

0.704
  radA Bacillus subtilis subsp. subtilis str. 168

62.637

99.781

0.625

Multiple sequence alignment