Detailed information NTG1135457

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: DQM39_RS01785
• Genome accession: NZ_LS483298
• Coordinates: 307535..308605 (+)
• Length: 356 a.a.
• NCBI ID: WP_002986002.1
• Uniprot ID: Q1JNF7
• Organism: Streptococcus pyogenes strain NCTC8225
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 302535..313605

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM39_RS01770 (NCTC8225_00360)	amiA	303063..305033 (+)	1971	WP_002986009.1	peptide ABC transporter substrate-binding protein	Regulator
DQM39_RS01775 (NCTC8225_00361)	amiC	305098..306600 (+)	1503	WP_002986006.1	ABC transporter permease	Regulator
DQM39_RS01780 (NCTC8225_00362)	amiD	306600..307526 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
DQM39_RS01785 (NCTC8225_00363)	amiE	307535..308605 (+)	1071	WP_002986002.1	ABC transporter ATP-binding protein	Regulator
DQM39_RS01790 (NCTC8225_00364)	amiF	308598..309521 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
DQM39_RS10345	-	309559..309648 (-)	90	WP_110002794.1	IS3 family transposase	-
DQM39_RS09930	-	309669..309914 (-)	246	WP_002985998.1	hypothetical protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39749.85 Da
• Isoelectric Point: 5.7015

>NTDB_id=1135457 DQM39_RS01785 WP_002986002.1 307535..308605(+) (amiE) [Streptococcus pyogenes strain NCTC8225]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGRQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=1135457 DQM39_RS01785 WP_002986002.1 307535..308605(+) (amiE) [Streptococcus pyogenes strain NCTC8225]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGACAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTAATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

• ABC transporter (ABC_tran) (29-187)

3D structure

• AlphaFold DB: Q1JNF7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.389	100	0.772
amiE	Streptococcus thermophilus LMG 18311	75.278	100	0.761
amiE	Streptococcus thermophilus LMD-9	75.278	100	0.761
oppD	Streptococcus mutans UA159	56.677	94.663	0.537