Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JM183_RS11070 Genome accession   NZ_LR962863
Coordinates   2314770..2316137 (-) Length   455 a.a.
NCBI ID   WP_126496070.1    Uniprot ID   -
Organism   Staphylococcus schleiferi strain NCTC12218     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2309770..2321137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM183_RS11045 (NCTC12218_02334) cysE 2309970..2310599 (-) 630 WP_171839470.1 serine O-acetyltransferase -
  JM183_RS11050 (NCTC12218_02336) gltX 2310986..2312440 (-) 1455 WP_016424287.1 glutamate--tRNA ligase -
  JM183_RS11055 ispF 2312517..2312997 (-) 481 Protein_2171 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  JM183_RS11060 (NCTC12218_02339) ispD 2312990..2313688 (-) 699 WP_016424285.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JM183_RS11065 (NCTC12218_02340) - 2313689..2314750 (-) 1062 WP_126496071.1 PIN/TRAM domain-containing protein -
  JM183_RS11070 (NCTC12218_02341) radA 2314770..2316137 (-) 1368 WP_126496070.1 DNA repair protein RadA Machinery gene
  JM183_RS11075 (NCTC12218_02343) - 2316573..2319033 (-) 2461 Protein_2175 ATP-dependent Clp protease ATP-binding subunit -
  JM183_RS11080 (NCTC12218_02344) - 2319051..2320055 (-) 1005 WP_016424281.1 protein arginine kinase -
  JM183_RS11085 (NCTC12218_02345) - 2320045..2320575 (-) 531 WP_016424280.1 UvrB/UvrC motif-containing protein -
  JM183_RS11090 (NCTC12218_02346) - 2320589..2321050 (-) 462 WP_016424279.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50007.54 Da        Isoelectric Point: 6.9560

>NTDB_id=1134921 JM183_RS11070 WP_126496070.1 2314770..2316137(-) (radA) [Staphylococcus schleiferi strain NCTC12218]
MAKKKVIFECTACGYQSPKWMGKCPNCGAWNTMEESIEQKVASPKHGVRSQKTSTSKIQKLNEIKQEQAPRILTNSDEFN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSQSKNVLYITGEESLNQTKLRADRLDEDASQLNVFAETDLEVIHEA
VKKIEPELIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLSIIIATASSFKDQAVDGLDCFIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVILPKTNIGGWQFPQGIEVIGVSTVNEALKYAIK

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1134921 JM183_RS11070 WP_126496070.1 2314770..2316137(-) (radA) [Staphylococcus schleiferi strain NCTC12218]
GTGGCGAAAAAGAAAGTGATATTTGAATGTACAGCTTGTGGTTATCAATCTCCAAAATGGATGGGCAAATGTCCAAATTG
TGGTGCATGGAATACAATGGAAGAATCGATAGAGCAAAAAGTAGCGAGCCCTAAACATGGGGTCAGATCTCAAAAAACGA
GTACTTCCAAAATTCAAAAACTAAATGAAATTAAACAAGAGCAAGCACCGCGTATCCTAACAAATAGTGATGAGTTTAAC
CGTGTACTTGGCGGAGGTATTGTAGAAGGATCGCTCGTTCTCATTGGTGGTGACCCAGGTATTGGGAAATCGACGTTGTT
ATTACAAATGTGTGCAGCATTGTCCCAAAGTAAAAATGTATTATATATTACAGGTGAGGAGTCACTTAATCAAACAAAGT
TAAGAGCAGATCGCCTAGACGAAGATGCGAGTCAATTGAATGTTTTTGCTGAGACAGACTTAGAAGTGATTCATGAAGCA
GTAAAGAAAATTGAACCGGAGCTTATTGTAGTGGACTCAATTCAAACGATTTATCATCCAGAGATTAGTTCGGCTCCAGG
CTCTGTGTCACAAGTTCGTGAAAGTACACAAAGCTTAATGCATATTGCAAAACAAATGAATATTGCTACTTTTATTGTAG
GGCATGTGACAAAAGAAGGCCAAATTGCTGGACCGCGTTTGTTAGAACACATGGTGGATACGGTGCTGTATTTCGAAGGC
GACGAACATCATGCTTATCGTATTTTAAGAGCCGTTAAAAACCGTTTTGGTTCCACGAATGAAATGGGCATATTCGAAAT
GAAACAGTCTGGATTGAAAAGTGTATTGAACCCATCAGAGATGTTTTTAGAAGAGCGTTCTACCAACGTACCTGGCTCAA
CAATCGTTGCGACGATGGAAGGGACACGTCCGCTACTTATTGAAGTTCAAGCGCTCGTTACGCCGACGACATTTAATAAC
CCTCGACGAATGGCTACAGGCATAGATCATAATCGTTTGAGTTTATTAATGGCAGTGTTAGAGAAAAAAGAAGGCTATCT
ATTACAACAACAAGACGCCTATATTAAAGTAGCAGGAGGCGTGAAGTTGTCTGAGCCAGCAGTGGATTTAAGTATTATTA
TCGCAACAGCATCAAGTTTTAAAGACCAAGCTGTCGATGGATTAGATTGCTTTATTGGAGAAGTTGGATTAACAGGTGAA
GTACGACGCGTTTCCCGTATCGAACAACGGGTACAAGAAGCGGCAAAACTCGGTTTTAAACGTGTGATATTACCTAAAAC
AAATATAGGGGGATGGCAATTCCCGCAAGGTATTGAAGTGATTGGTGTTTCTACTGTAAATGAAGCATTAAAATATGCCA
TAAAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.886

100

0.67
  radA Streptococcus pneumoniae Rx1

60.31

99.121

0.598
  radA Streptococcus pneumoniae D39

60.31

99.121

0.598
  radA Streptococcus pneumoniae R6

60.31

99.121

0.598
  radA Streptococcus pneumoniae TIGR4

60.31

99.121

0.598
  radA Streptococcus mitis NCTC 12261

60.31

99.121

0.598
  radA Streptococcus mitis SK321

60.31

99.121

0.598

Multiple sequence alignment