Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NMG84_RS00440 Genome accession   NZ_LR882963
Coordinates   108758..110761 (-) Length   667 a.a.
NCBI ID   WP_042153548.1    Uniprot ID   A0A1J1JPF8
Organism   Planktothrix agardhii strain No.66     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103758..115761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMG84_RS00420 (PANO66_00088) - 104823..105413 (+) 591 WP_042153557.1 DUF4126 domain-containing protein -
  NMG84_RS00425 (PANO66_00089) - 105438..106046 (-) 609 WP_042153554.1 hypothetical protein -
  NMG84_RS00430 (PANO66_00090) - 106212..107345 (-) 1134 WP_227350428.1 type II secretion system F family protein -
  NMG84_RS00435 (PANO66_00091) - 107473..108573 (-) 1101 WP_042153550.1 type IV pilus twitching motility protein PilT -
  NMG84_RS00440 (PANO66_00092) pilB/pilB1 108758..110761 (-) 2004 WP_042153548.1 GspE/PulE family protein Machinery gene
  NMG84_RS00445 (PANO66_00093) - 110776..111168 (-) 393 WP_193372725.1 hypothetical protein -
  NMG84_RS00450 (PANO66_00094) grpE 111522..112256 (+) 735 WP_042153542.1 nucleotide exchange factor GrpE -
  NMG84_RS00455 (PANO66_00095) dnaK 112461..114542 (+) 2082 WP_227350427.1 molecular chaperone DnaK -
  NMG84_RS00460 (PANO66_00096) dnaJ 114561..115691 (+) 1131 WP_026796142.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74881.77 Da        Isoelectric Point: 5.2090

>NTDB_id=1132941 NMG84_RS00440 WP_042153548.1 108758..110761(-) (pilB/pilB1) [Planktothrix agardhii strain No.66]
MTNTSQRRALVVQSNTPFGNKLIQSGYINPEQLQQAIKTARPGQPLTEILESITGQPLPPELARQYKKQQLFELRIIHGV
EAYDPEIEPMTTEELEAMLKFLETSLETCKRHRFIPLRKVESDPSSVLIAMVDPDNLTALEELRNQILRPQALKLQRRVI
THEDYDQIISAYSDEDVKRKAVEAAIRDKEKLDFTAGDFDTTDLEDMGDEGGGMDLADEIEQSETAPVIKAVNVILAKAL
SEGVSDIHVEPQEHEMRIRMRRDGVLEEYYKFPKKVIPAITSRFKILAEMDIAERRQAQDGRIRRKFRGRTIDFRVNSLP
SRYGEKIVLRILDSSSTQLGLDKLITDQETLALVREIASRPFGLILVTGPTGSGKSTSLYSVLAERNDPAINISTAEDPI
EYALPGITQCQVIREKNLTFANILRAFLRQDPDVLLVGETRDLETAKTSIEAALTGHLVLTTLHTNDAAGAIARLDEMGV
EPFMVSAALLGILAQRLMRRVCGECRIPYTPTVEELGRYGLSASQEVDITFYKANTIPIEQRKHLADSGQLCKKCNGLGY
KGRVGVYEFLKVTERLQTLITKGAPTEQIKEAAVEEGMKTILAYSLQLVREGYTTFEEVERVTFTDSGLEAELKAKRKQG
LTCKSCEAELKPEWLDCPYCMTPRFTS

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=1132941 NMG84_RS00440 WP_042153548.1 108758..110761(-) (pilB/pilB1) [Planktothrix agardhii strain No.66]
ATGACTAATACATCCCAAAGACGCGCCTTGGTTGTTCAAAGTAATACTCCCTTTGGCAACAAATTAATTCAATCTGGTTA
TATTAATCCTGAGCAGTTGCAGCAGGCTATAAAAACAGCCCGCCCCGGTCAGCCCCTAACGGAAATTTTAGAATCGATCA
CGGGTCAGCCCCTGCCCCCGGAGTTAGCCCGTCAGTACAAAAAACAACAGTTATTTGAACTGAGAATTATTCATGGGGTC
GAAGCCTATGATCCTGAAATTGAACCGATGACGACGGAAGAACTCGAAGCCATGCTGAAATTTCTGGAGACAAGTTTAGA
AACCTGTAAACGCCATCGCTTTATTCCCCTACGCAAGGTCGAAAGTGATCCATCTTCGGTCTTAATTGCGATGGTTGACC
CCGATAACCTGACGGCGTTGGAGGAGTTAAGAAACCAAATTCTACGACCCCAAGCCCTGAAGTTACAACGGCGGGTAATT
ACCCATGAAGATTACGATCAAATCATTTCCGCCTACAGTGATGAAGATGTTAAACGCAAAGCCGTCGAAGCTGCCATTCG
TGACAAAGAAAAATTAGATTTTACCGCCGGGGATTTTGACACCACAGACCTCGAAGATATGGGGGATGAAGGCGGTGGCA
TGGATTTGGCCGATGAAATCGAACAATCGGAAACCGCCCCGGTGATCAAAGCGGTGAACGTGATTTTAGCCAAAGCTCTC
TCGGAAGGGGTGTCTGATATTCACGTCGAACCCCAAGAACATGAGATGCGGATTCGGATGCGTCGAGATGGGGTTTTAGA
AGAATATTATAAGTTTCCGAAAAAGGTGATTCCCGCCATTACCTCCCGGTTTAAGATTCTAGCGGAAATGGATATCGCCG
AACGCCGTCAAGCCCAGGATGGTCGGATCCGTCGGAAGTTTCGAGGACGGACGATCGATTTTCGGGTGAATAGTCTCCCC
AGTCGCTACGGGGAAAAAATTGTTTTGCGGATTTTGGATAGTTCCTCAACTCAATTAGGGTTAGATAAATTAATTACTGA
TCAAGAAACCTTAGCCTTAGTTCGGGAAATCGCTTCTCGACCCTTTGGGTTGATTTTAGTCACCGGGCCAACGGGTTCAG
GAAAATCCACCTCCTTGTATTCGGTTTTAGCCGAACGCAACGATCCAGCGATTAATATTAGTACGGCCGAAGATCCGATT
GAATATGCCTTACCCGGAATTACCCAGTGTCAGGTAATTCGAGAGAAAAATTTAACTTTTGCTAATATTTTACGGGCGTT
TTTACGACAAGATCCCGATGTTTTATTGGTGGGGGAAACGCGAGATTTAGAAACGGCCAAGACTTCTATTGAAGCCGCCT
TAACCGGACACTTAGTATTAACTACTTTACACACAAACGACGCGGCTGGGGCGATCGCCCGTTTGGATGAAATGGGGGTG
GAACCCTTCATGGTGTCAGCCGCCTTATTAGGGATTCTCGCCCAACGTCTGATGCGTCGGGTTTGTGGCGAGTGTCGGAT
TCCCTATACCCCCACGGTTGAAGAACTCGGTCGTTATGGACTTTCCGCTTCCCAAGAAGTTGATATTACCTTCTATAAAG
CCAATACCATTCCTATTGAACAACGGAAACATCTGGCGGATTCGGGTCAGTTGTGCAAAAAATGTAATGGACTGGGTTAC
AAAGGTCGGGTAGGGGTGTACGAATTCTTGAAGGTGACTGAAAGACTGCAAACCCTAATTACCAAAGGTGCCCCCACGGA
ACAAATTAAAGAAGCTGCTGTAGAAGAAGGGATGAAAACCATCTTAGCCTACAGTTTGCAGCTAGTCCGCGAAGGCTATA
CCACCTTTGAAGAAGTGGAGCGGGTGACGTTTACCGATTCTGGTTTAGAAGCGGAACTCAAAGCCAAACGAAAACAGGGC
TTAACCTGTAAGAGTTGTGAAGCGGAATTGAAACCGGAATGGTTAGATTGTCCCTATTGCATGACTCCACGCTTTACTAG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(22-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J1JPF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.596

99.55

0.613

Multiple sequence alignment