Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EO53_RS01070 Genome accession   NZ_CP009685
Coordinates   233938..234615 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228938..239615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO53_RS01055 (EO53_01055) chiA 230305..232998 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  EO53_RS01060 (EO53_01060) bfd 233167..233361 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  EO53_RS01065 (EO53_01065) bfr 233433..233909 (+) 477 WP_000675504.1 bacterioferritin -
  EO53_RS01070 (EO53_01070) pilD 233938..234615 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  EO53_RS01075 (EO53_01075) gspM 234615..235076 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  EO53_RS01080 (EO53_01080) gspL 235073..236236 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  EO53_RS01085 (EO53_01085) gspK 236251..237234 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  EO53_RS01090 (EO53_01090) gspJ 237227..237814 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  EO53_RS01095 (EO53_01095) gspI 237807..238184 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  EO53_RS01100 (EO53_01100) gspH 238181..238690 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  EO53_RS25000 (EO53_01105) gspG 238698..239135 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=113274 EO53_RS01070 WP_000178154.1 233938..234615(-) (pilD) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=113274 EO53_RS01070 WP_000178154.1 233938..234615(-) (pilD) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment