Detailed information NTG1132341

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: H1W81_RS05265
• Genome accession: NZ_LR824002
• Coordinates: 1012768..1013649 (+)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus isolate STH_CIRM_67
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1007768..1018649

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1W81_RS05240 (STHERMO_1145)	-	1009268..1009723 (+)	456	WP_041827047.1	DUF4832 domain-containing protein	-
H1W81_RS05245 (STHERMO_1146)	galE	1009738..1010751 (+)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
H1W81_RS05250 (STHERMO_1147)	-	1010939..1011175 (+)	237	WP_179971627.1	DUF1146 family protein	-
H1W81_RS05255 (STHERMO_1148)	murA	1011245..1012516 (+)	1272	WP_041828250.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
H1W81_RS05260 (STHERMO_1149)	-	1012518..1012706 (+)	189	WP_011226090.1	DNA-directed RNA polymerase subunit beta	-
H1W81_RS05265 (STHERMO_1150)	endA	1012768..1013649 (+)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
H1W81_RS05270 (STHERMO_1151)	-	1013720..1014370 (-)	651	WP_011226088.1	amino acid ABC transporter permease	-
H1W81_RS05275 (STHERMO_1152)	-	1014382..1015080 (-)	699	WP_011226087.1	amino acid ABC transporter permease	-
H1W81_RS05280 (STHERMO_1153)	-	1015098..1015931 (-)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
H1W81_RS05285 (STHERMO_1154)	-	1015943..1016710 (-)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
H1W81_RS05290 (STHERMO_1156)	vicR	1017242..1017949 (+)	708	WP_011227274.1	response regulator YycF	Regulator

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=1132341 H1W81_RS05265 WP_011226089.1 1012768..1013649(+) (endA) [Streptococcus thermophilus isolate STH_CIRM_67]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=1132341 H1W81_RS05265 WP_011226089.1 1012768..1013649(+) (endA) [Streptococcus thermophilus isolate STH_CIRM_67]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594