Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   H1W81_RS02250 Genome accession   NZ_LR824002
Coordinates   407522..408883 (+) Length   453 a.a.
NCBI ID   WP_082302317.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_67     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 402522..413883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W81_RS02225 (STHERMO_0461) - 403601..404174 (-) 574 Protein_382 MptD family putative ECF transporter S component -
  H1W81_RS02230 - 404183..404973 (-) 791 Protein_383 formate/nitrite transporter family protein -
  H1W81_RS02235 (STHERMO_0464) - 405091..406061 (-) 971 Protein_384 PAS domain-containing protein -
  H1W81_RS02240 - 406089..406684 (-) 596 Protein_385 NADPH-dependent FMN reductase -
  H1W81_RS02245 (STHERMO_0468) - 406898..407344 (+) 447 WP_002947069.1 dUTP diphosphatase -
  H1W81_RS09945 - 407345..407486 (+) 142 Protein_387 cysteine hydrolase -
  H1W81_RS02250 (STHERMO_0469) radA 407522..408883 (+) 1362 WP_082302317.1 DNA repair protein RadA Machinery gene
  H1W81_RS02255 - 408972..409647 (+) 676 Protein_389 AIM24 family protein -
  H1W81_RS02260 (STHERMO_0474) - 409815..410312 (+) 498 WP_002947059.1 beta-class carbonic anhydrase -
  H1W81_RS02265 (STHERMO_0475) - 410404..410757 (-) 354 WP_002953536.1 DUF3397 domain-containing protein -
  H1W81_RS02270 (STHERMO_0476) rplK 410929..411354 (+) 426 WP_002953535.1 50S ribosomal protein L11 -
  H1W81_RS02275 (STHERMO_0477) rplA 411454..412143 (+) 690 WP_002946412.1 50S ribosomal protein L1 -
  H1W81_RS02280 - 412262..413289 (+) 1028 Protein_394 peptide ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49450.86 Da        Isoelectric Point: 6.6528

>NTDB_id=1132324 H1W81_RS02250 WP_082302317.1 407522..408883(+) (radA) [Streptococcus thermophilus isolate STH_CIRM_67]
MAKKKSTFVCRECGYNSPKYLGRCPNCSSWSSFEEEVEMQEVKNARVSLTGEKSKPTKLKEVSSISYARIKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAVRSQVE
GIKPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELIQIAKTNNIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKELPTNPQEAFVGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYVPKNSLHGMKIPEGIQVIGVTTVGEVLKKVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1132324 H1W81_RS02250 WP_082302317.1 407522..408883(+) (radA) [Streptococcus thermophilus isolate STH_CIRM_67]
ATAGCTAAGAAAAAATCAACTTTTGTTTGTAGAGAATGTGGCTACAATTCACCCAAATATCTAGGGCGCTGTCCCAATTG
TTCTTCTTGGTCCTCTTTTGAGGAAGAGGTTGAGATGCAGGAGGTTAAAAATGCCCGTGTCAGTTTGACGGGTGAAAAGT
CCAAACCTACTAAGTTAAAAGAAGTCTCTTCAATTAGTTATGCCCGCATAAAAACCGATATGGACGAGTTTAACCGTGTT
TTAGGTGGCGGTGTGGTTCCGGGGAGTTTGGTTCTTATTGGAGGAGATCCAGGAATAGGGAAATCGACCCTCCTCTTACA
GGTGTCAACACAGCTGGCTAACAAGGGAACTGTCCTCTATGTTTCTGGGGAAGAATCGGCCGAGCAAATCAAATTGCGGA
GTGAACGTTTAGGTGATATTGATAACGAATTTTATCTGTATGCTGAGACCAATATGCAAGCAGTCCGTAGTCAAGTGGAG
GGTATCAAACCCGATTTCCTCATTATTGATTCTATTCAGACCATCATGAGCCCAGAGATCTCTGGCGTTCAAGGTTCTGT
TTCCCAGGTTCGTGAAGTAACTGCCGAACTCATACAGATTGCCAAAACCAATAACATTGCCACTTTCATTGTTGGGCATG
TGACCAAGGAGGGACAGCTGGCTGGACCACGTATGTTGGAGCACATGGTGGATACTGTGCTTTATTTTGAGGGGGAACGT
CATCATACCTTCCGTATTCTGAGGGCAGTTAAAAACCGTTTTGGTTCAACCAATGAGATTGGGATTTTTGAAATGCAGTC
AGGTGGTCTGGTCGAGGTACTAAACCCTAGCCAAGTTTTCTTAGAAGAACGCCTAGACGGTGCCACAGGTTCTGCCATTG
TTGTCACAATGGAGGGGAGTCGACCTATCCTAGCTGAGGTTCAAGCTTTGGTGACACCGACCGTCTTTGGTAACGCAAAG
CGGACAACGACGGGACTGGACTTTAACCGTGTCAGCCTTATCATGGCTGTCTTAGAGAAACGTTGCGGCCTTCTTCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAGTTGGACGAGCCAGCTATTGACCTTGCAGTTGCCGTTGCTA
TCGCTTCTAGTTACAAGGAGCTTCCAACCAATCCACAAGAAGCCTTCGTAGGAGAAATTGGCTTGACCGGAGAGATTCGT
CGTGTGACTCGTATTGAGCAGCGTATCAATGAAGCCGCTAAACTAGGTTTTACTAAAATTTATGTTCCTAAAAATTCGCT
TCATGGCATGAAGATTCCAGAAGGTATCCAGGTCATTGGGGTCACAACGGTAGGAGAAGTACTCAAAAAAGTCTTTTCTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.742

100

0.887
  radA Streptococcus pneumoniae D39

88.742

100

0.887
  radA Streptococcus pneumoniae R6

88.742

100

0.887
  radA Streptococcus pneumoniae TIGR4

88.742

100

0.887
  radA Streptococcus mitis NCTC 12261

88.742

100

0.887
  radA Streptococcus mitis SK321

88.742

100

0.887
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment