Detailed information    

insolico Bioinformatically predicted

Overview


Name   amiF   Type   Regulator
Locus tag   H1W87_RS06655 Genome accession   NZ_LR822037
Coordinates   1272659..1273588 (-) Length   309 a.a.
NCBI ID   WP_002951426.1    Uniprot ID   A0A7U7CIU5
Organism   Streptococcus thermophilus isolate STH_CIRM_1121     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1257123..1327202 1272659..1273588 within 0


Gene organization within MGE regions


Location: 1257123..1327202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W87_RS06580 (STHERMO_1466) liaF 1257131..1257829 (-) 699 WP_011226289.1 cell wall-active antibiotics response protein LiaF -
  H1W87_RS09765 (STHERMO_1467) - 1258084..1258251 (-) 168 WP_232088837.1 potassium channel family protein -
  H1W87_RS06590 (STHERMO_1470) stkP/pknB 1258888..1260759 (-) 1872 WP_180478115.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  H1W87_RS06595 (STHERMO_1471) - 1260759..1261496 (-) 738 WP_180478117.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  H1W87_RS06600 (STHERMO_1472) rsmB 1261540..1262862 (-) 1323 WP_014608533.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  H1W87_RS06605 (STHERMO_1473) fmt 1262852..1263787 (-) 936 WP_002951411.1 methionyl-tRNA formyltransferase -
  H1W87_RS06610 (STHERMO_1474) - 1263805..1266201 (-) 2397 WP_180478119.1 primosomal protein N' -
  H1W87_RS06615 (STHERMO_1475) rpoZ 1266343..1266657 (-) 315 WP_002951415.1 DNA-directed RNA polymerase subunit omega -
  H1W87_RS06620 (STHERMO_1476) gmk 1266679..1267308 (-) 630 WP_022096878.1 guanylate kinase -
  H1W87_RS06625 (STHERMO_1477) ftsY 1267541..1268932 (-) 1392 WP_180478121.1 signal recognition particle-docking protein FtsY -
  H1W87_RS06630 (STHERMO_1478) - 1268946..1269761 (-) 816 WP_024010001.1 Cof-type HAD-IIB family hydrolase -
  H1W87_RS06635 (STHERMO_1479) - 1269754..1270548 (-) 795 WP_180478123.1 HAD-IIB family hydrolase -
  H1W87_RS06640 (STHERMO_1481) - 1270732..1271109 (+) 378 WP_011226300.1 GntR family transcriptional regulator -
  H1W87_RS06645 (STHERMO_1482) - 1271114..1271812 (+) 699 WP_011226301.1 ABC transporter ATP-binding protein -
  H1W87_RS06650 (STHERMO_1483) - 1271824..1272609 (+) 786 WP_002951425.1 hypothetical protein -
  H1W87_RS06655 (STHERMO_1484) amiF 1272659..1273588 (-) 930 WP_002951426.1 ATP-binding cassette domain-containing protein Regulator
  H1W87_RS06660 (STHERMO_1485) amiE 1273581..1274666 (-) 1086 WP_011226304.1 ABC transporter ATP-binding protein Regulator
  H1W87_RS06665 (STHERMO_1486) amiD 1274676..1275602 (-) 927 WP_002946409.1 oligopeptide ABC transporter permease OppC Regulator
  H1W87_RS06670 (STHERMO_1487) amiC 1275602..1277095 (-) 1494 WP_002946410.1 ABC transporter permease Regulator
  H1W87_RS06675 - 1277157..1279123 (-) 1967 Protein_1261 peptide ABC transporter substrate-binding protein -
  H1W87_RS09370 (STHERMO_1490) - 1279479..1280710 (+) 1232 Protein_1262 ISL3 family transposase -
  H1W87_RS06685 (STHERMO_1491) amiA3 1280755..1282728 (-) 1974 WP_011226306.1 peptide ABC transporter substrate-binding protein Regulator
  H1W87_RS06690 - 1282932..1283122 (-) 191 Protein_1264 IS3 family transposase -
  H1W87_RS09770 - 1283116..1283526 (-) 411 Protein_1265 ATP-binding cassette domain-containing protein -
  H1W87_RS06705 (STHERMO_1494) - 1283558..1283872 (-) 315 Protein_1266 TatD family hydrolase -
  H1W87_RS06710 (STHERMO_1495) - 1284219..1285424 (+) 1206 WP_011227415.1 OFA family MFS transporter -
  H1W87_RS06715 - 1285471..1286824 (-) 1354 Protein_1268 IS3 family transposase -
  H1W87_RS06720 (STHERMO_1501) pta 1286910..1287893 (-) 984 WP_011227420.1 phosphate acetyltransferase -
  H1W87_RS06725 (STHERMO_1502) - 1287903..1288802 (-) 900 WP_180478127.1 RluA family pseudouridine synthase -
  H1W87_RS06730 (STHERMO_1503) - 1288799..1289635 (-) 837 WP_096811572.1 NAD kinase -
  H1W87_RS06735 (STHERMO_1504) - 1289607..1290281 (-) 675 WP_002951443.1 GTP pyrophosphokinase family protein -
  H1W87_RS06740 (STHERMO_1505) - 1290377..1290952 (+) 576 WP_002951444.1 CYTH domain-containing protein -
  H1W87_RS06745 (STHERMO_1507) - 1291317..1292288 (+) 972 WP_002951446.1 ribose-phosphate diphosphokinase -
  H1W87_RS06750 (STHERMO_1508) - 1292292..1293404 (+) 1113 WP_180478129.1 cysteine desulfurase family protein -
  H1W87_RS06755 (STHERMO_1509) - 1293406..1293753 (+) 348 WP_014608555.1 DUF1831 domain-containing protein -
  H1W87_RS06760 (STHERMO_1510) - 1293897..1294556 (+) 660 WP_173940607.1 redox-sensing transcriptional repressor Rex -
  H1W87_RS06765 (STHERMO_1511) - 1294549..1295244 (+) 696 WP_002951449.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  H1W87_RS06770 (STHERMO_1512) radC 1295297..1295983 (-) 687 WP_180478131.1 RadC family protein -
  H1W87_RS06775 (STHERMO_1513) - 1296032..1297816 (-) 1785 WP_011226325.1 rhamnan synthesis F family protein -
  H1W87_RS06780 (STHERMO_1514) - 1297813..1298868 (-) 1056 WP_096811576.1 glycosyltransferase family 2 protein -
  H1W87_RS06785 (STHERMO_1515) - 1298888..1300093 (-) 1206 WP_014727633.1 ABC transporter ATP-binding protein -
  H1W87_RS06790 (STHERMO_1516) - 1300093..1300902 (-) 810 WP_002948020.1 ABC transporter permease -
  H1W87_RS06795 (STHERMO_1517) - 1300886..1301842 (-) 957 WP_011226328.1 glycosyltransferase family 2 protein -
  H1W87_RS06800 (STHERMO_1518) cps2T 1301839..1302987 (-) 1149 WP_180478133.1 beta 1-4 rhamnosyltransferase Cps2T -
  H1W87_RS06805 (STHERMO_1519) - 1303096..1304106 (-) 1011 WP_011226330.1 glycosyltransferase family 2 protein -
  H1W87_RS06810 (STHERMO_1520) - 1304103..1305380 (-) 1278 WP_180478136.1 lipopolysaccharide biosynthesis protein -
  H1W87_RS06815 (STHERMO_1521) - 1305373..1305705 (-) 333 WP_011226332.1 DUF2304 domain-containing protein -
  H1W87_RS06820 (STHERMO_1522) - 1305711..1306406 (-) 696 WP_171073564.1 glycosyltransferase family 2 protein -
  H1W87_RS06825 (STHERMO_1523) rfbD 1306483..1307334 (-) 852 WP_014621820.1 dTDP-4-dehydrorhamnose reductase -
  H1W87_RS06830 (STHERMO_1524) - 1307411..1308745 (-) 1335 WP_002946422.1 DUF6056 family protein -
  H1W87_RS06835 (STHERMO_1525) - 1308858..1309775 (-) 918 WP_375708554.1 glycosyltransferase family 2 protein -
  H1W87_RS06840 (STHERMO_1526) - 1309838..1311253 (-) 1416 WP_096811580.1 DUF2142 domain-containing protein -
  H1W87_RS06845 (STHERMO_1528) - 1311587..1311922 (-) 336 WP_002891474.1 metal-sulfur cluster assembly factor -
  H1W87_RS06850 (STHERMO_1529) rpoD 1311976..1313085 (-) 1110 WP_011226338.1 RNA polymerase sigma factor RpoD -
  H1W87_RS06855 (STHERMO_1530) dnaG 1313089..1314900 (-) 1812 WP_011681448.1 DNA primase -
  H1W87_RS06860 (STHERMO_1531) mscL 1315041..1315124 (+) 84 Protein_1297 large conductance mechanosensitive channel protein MscL -
  H1W87_RS06865 (STHERMO_1532) rpsU 1315284..1315460 (-) 177 WP_082309068.1 30S ribosomal protein S21 -
  H1W87_RS06870 (STHERMO_1533) - 1315655..1316449 (-) 795 WP_023909836.1 ABC transporter substrate-binding protein -
  H1W87_RS06875 (STHERMO_1534) - 1316542..1317651 (-) 1110 WP_041828295.1 aminotransferase -
  H1W87_RS06880 (STHERMO_1535) - 1317998..1318795 (-) 798 WP_179966739.1 transporter substrate-binding domain-containing protein -
  H1W87_RS06885 (STHERMO_1536) - 1318792..1319619 (-) 828 WP_180478138.1 transporter substrate-binding domain-containing protein -
  H1W87_RS06890 (STHERMO_1537) - 1319832..1320296 (-) 465 WP_011226345.1 8-oxo-dGTP diphosphatase -
  H1W87_RS06895 (STHERMO_1538) uvrB 1320341..1322332 (-) 1992 WP_180478140.1 excinuclease ABC subunit UvrB -
  H1W87_RS06905 - 1323019..1323955 (-) 937 Protein_1305 CPBP family intramembrane glutamic endopeptidase -
  H1W87_RS06910 (STHERMO_1543) - 1324154..1326364 (+) 2211 WP_180478144.1 ABC transporter substrate-binding protein/permease -
  H1W87_RS06915 (STHERMO_1544) - 1326364..1327104 (+) 741 WP_002945131.1 amino acid ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 309 a.a.        Molecular weight: 35171.28 Da        Isoelectric Point: 6.5989

>NTDB_id=1131892 H1W87_RS06655 WP_002951426.1 1272659..1273588(-) (amiF) [Streptococcus thermophilus isolate STH_CIRM_1121]
MPEKLVEVKDVEISFGEGRKKFVAVHNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSNGEIIFDGKKINGYLSHS
EKNDLIRRIQMIFQDPAASLNERATVDYILSEGLYNFHLYKDEEERKAKIKEIIKEVGLLEEHLTRYPHEFSGGQRQRIG
IARSLVMQPDLVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGTIVEVAETEELYN
NPIHPYTKSLLSAVPIPDPILERKKVLKVYDPNQHDYSVDKPEMVEVRPGHFVWGNKTEIETYRKEQSK

Nucleotide


Download         Length: 930 bp        

>NTDB_id=1131892 H1W87_RS06655 WP_002951426.1 1272659..1273588(-) (amiF) [Streptococcus thermophilus isolate STH_CIRM_1121]
ATGCCTGAGAAATTAGTTGAAGTAAAAGATGTGGAAATTTCCTTCGGCGAAGGAAGAAAGAAGTTCGTTGCTGTCCACAA
TGCTAATTTTTTCATCAACAAGGGTGAAACCTTCTCCCTCGTTGGTGAGTCTGGTAGTGGTAAAACGACTATTGGACGTG
CCATTATCGGTTTAAATGACACAAGTAATGGTGAGATTATTTTTGACGGTAAGAAGATCAATGGATACTTATCTCACTCT
GAGAAAAACGACCTTATCCGTCGTATTCAGATGATTTTCCAAGACCCTGCGGCTAGTTTGAATGAACGTGCGACAGTCGA
TTATATCTTGTCTGAGGGCTTGTACAATTTCCATCTTTATAAAGATGAGGAAGAACGTAAGGCTAAAATCAAGGAAATCA
TCAAAGAAGTAGGACTTCTTGAGGAGCACTTAACACGTTACCCTCACGAATTTTCTGGGGGACAACGTCAACGTATCGGG
ATTGCGCGTTCTTTGGTCATGCAGCCTGATTTGGTTATCGCTGATGAACCAATCTCAGCCCTTGACGTGTCAGTTCGTGC
CCAAGTTTTGAATTTGCTTAAGAAATTCCAAAAAGAGTTGGGGTTAACCTATCTCTTTATCGCTCACGATTTGTCAGTGG
TCCGTTTCATTTCTGACCGTATCGCTGTTATCTATAAGGGGACAATCGTGGAAGTTGCTGAGACAGAAGAGCTCTACAAC
AATCCTATCCATCCTTACACCAAGTCACTCTTGTCTGCTGTTCCTATTCCAGATCCAATCTTGGAACGTAAGAAAGTCTT
GAAGGTTTATGATCCAAACCAACACGACTATTCGGTTGATAAACCAGAAATGGTGGAAGTACGCCCAGGTCACTTCGTTT
GGGGTAACAAGACAGAAATTGAGACTTATCGTAAAGAACAAAGTAAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A7U7CIU5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMG 18311

100

100

1

  amiF Streptococcus thermophilus LMD-9

99.676

100

0.997

  amiF Streptococcus salivarius strain HSISS4

98.058

100

0.981


Multiple sequence alignment