Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M942_RS03145 Genome accession   NZ_CP006580
Coordinates   635602..637035 (-) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain P101     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 630602..642035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M942_RS03115 (M942_03090) elbB 632135..632788 (+) 654 WP_025202921.1 isoprenoid biosynthesis glyoxalase ElbB -
  M942_RS03120 (M942_03095) mtgA 632785..633510 (+) 726 WP_025202922.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  M942_RS03125 (M942_03100) npr 633549..633821 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  M942_RS03130 (M942_03105) rapZ 633818..634672 (-) 855 WP_014171820.1 RNase adapter RapZ -
  M942_RS03135 (M942_03110) ptsN 634718..635209 (-) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  M942_RS03140 (M942_03115) hpf 635292..635579 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  M942_RS03145 (M942_03120) rpoN/rpoN1 635602..637035 (-) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  M942_RS03150 (M942_03125) lptB 637083..637808 (-) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  M942_RS03155 (M942_03130) lptA 637815..638369 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  M942_RS03160 (M942_03135) lptC 638338..638913 (-) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  M942_RS03165 (M942_03140) kdsC 638910..639476 (-) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  M942_RS03170 (M942_03145) kdsD 639491..640477 (-) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  M942_RS03175 (M942_03150) - 640491..641468 (-) 978 WP_020883528.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=113185 M942_RS03145 WP_014171817.1 635602..637035(-) (rpoN/rpoN1) [Enterobacter ludwigii strain P101]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=113185 M942_RS03145 WP_014171817.1 635602..637035(-) (rpoN/rpoN1) [Enterobacter ludwigii strain P101]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATCGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAACTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCGGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTTGAACAGCAGCAGGCGTTCTTTGAGCAGGGTGAAGAGTATATGAAACCGATGGTGTTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486

Multiple sequence alignment