Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DENOEST_RS18075 Genome accession   NZ_LR778301
Coordinates   3958575..3960011 (-) Length   478 a.a.
NCBI ID   WP_145770419.1    Uniprot ID   A0A6S6Y0W3
Organism   Denitratisoma oestradiolicum strain DSM 16959     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3953575..3965011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DENOEST_RS18040 (DENOEST_3782) - 3953991..3955193 (+) 1203 WP_145770425.1 alanine--glyoxylate aminotransferase family protein -
  DENOEST_RS18045 (DENOEST_3783) - 3955203..3956099 (+) 897 WP_145770424.1 adenylate/guanylate cyclase domain-containing protein -
  DENOEST_RS18050 (DENOEST_3784) - 3956081..3956257 (-) 177 WP_170228167.1 hypothetical protein -
  DENOEST_RS18055 (DENOEST_3785) - 3956286..3956600 (-) 315 WP_145770423.1 PsiF family protein -
  DENOEST_RS18060 (DENOEST_3786) hprK 3956731..3957675 (-) 945 WP_145770422.1 HPr(Ser) kinase/phosphatase -
  DENOEST_RS18065 (DENOEST_3787) ptsN 3957660..3958118 (-) 459 WP_145770421.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DENOEST_RS18070 (DENOEST_3788) hpf 3958249..3958563 (-) 315 WP_145770420.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DENOEST_RS18075 (DENOEST_3789) rpoN/rpoN1 3958575..3960011 (-) 1437 WP_145770419.1 RNA polymerase factor sigma-54 Machinery gene
  DENOEST_RS18080 (DENOEST_3790) lptB 3960014..3960736 (-) 723 WP_145770418.1 LPS export ABC transporter ATP-binding protein -
  DENOEST_RS18085 (DENOEST_3791) recX 3960959..3961393 (-) 435 WP_145770417.1 recombination regulator RecX -
  DENOEST_RS18090 (DENOEST_3792) recA 3961390..3962415 (-) 1026 WP_145770416.1 recombinase RecA Machinery gene
  DENOEST_RS18095 (DENOEST_3793) - 3962443..3962955 (-) 513 WP_145770415.1 CinA family protein -
  DENOEST_RS18100 (DENOEST_3794) - 3962955..3963458 (-) 504 WP_183148284.1 phosphatidylglycerophosphatase A -
  DENOEST_RS18105 (DENOEST_3795) - 3963506..3963994 (+) 489 WP_145770414.1 hypothetical protein -
  DENOEST_RS18110 (DENOEST_3796) thiL 3963960..3964982 (-) 1023 WP_145770413.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 53157.29 Da        Isoelectric Point: 5.1722

>NTDB_id=1130271 DENOEST_RS18075 WP_145770419.1 3958575..3960011(-) (rpoN/rpoN1) [Denitratisoma oestradiolicum strain DSM 16959]
MKQTLQLKLSQHLALTPQLQQSIRLLQLSTLELNQEIEQFLQENPLLERVEPGDDGAAFAPPADLLTQTTTPEAPPERDD
TPPGPEGDEPGWGSELPMGHGNNRDPDDDSDAGDIQAAATSLRDHLLTQMALTQVSARERALVAFLVEALDEDGYLTQSL
EDLLDLLPEDFGGDREEQLEELQIALRHLQHFDPAGIGARNPRECLELQLLARPDCPAKELARTLVRDHLDQLAARDFIR
IKKALNCSDDELRDAQALILTLNPRPGAQYAALDTRYIVPDVVVRKLRGQWLASLNGEAMPRLRINRLYANILRQNRGSS
LAGHLQEAKWLIKNVQQRFDTILRVTQAIVDRQRAFFDHGEVAMRPLTLREIADTVGLHESTISRVTTQKYLASPRGIFE
LKYFFGSHVATDSGGAASSTAIRALIKQLVGAENGKKPLSDSRLAEILGEQGIVVARRTVAKYRESLNIPPVNLRKTL

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=1130271 DENOEST_RS18075 WP_145770419.1 3958575..3960011(-) (rpoN/rpoN1) [Denitratisoma oestradiolicum strain DSM 16959]
ATGAAACAGACGCTTCAGCTCAAGCTCTCCCAACACCTGGCCCTGACGCCCCAGTTGCAACAGTCGATCCGGCTGCTGCA
ACTGTCCACGCTGGAACTCAACCAGGAAATCGAGCAGTTCCTTCAGGAAAATCCCCTGCTGGAACGTGTTGAGCCGGGCG
ACGACGGTGCCGCCTTCGCCCCCCCCGCGGACCTTCTTACCCAGACCACAACCCCCGAAGCGCCTCCCGAGCGGGATGAC
ACGCCCCCTGGCCCGGAAGGCGACGAACCCGGCTGGGGCAGTGAGCTGCCCATGGGCCATGGCAACAACCGGGATCCGGA
TGACGACAGCGATGCCGGGGATATTCAGGCCGCAGCCACCAGCCTGCGGGATCACCTGCTGACCCAGATGGCCCTGACCC
AGGTCAGCGCCCGGGAGCGGGCTCTGGTGGCCTTTCTGGTGGAGGCTCTGGACGAGGATGGCTACCTGACCCAGTCCCTG
GAGGATTTGCTGGATCTGCTGCCTGAGGATTTCGGCGGTGACCGGGAGGAGCAACTGGAGGAATTGCAGATCGCCCTGCG
CCATTTGCAGCATTTCGATCCGGCGGGTATCGGTGCCCGCAACCCGCGGGAGTGCCTGGAGCTCCAACTGTTGGCCCGGC
CCGACTGTCCGGCCAAGGAACTCGCCCGGACTCTCGTCAGGGATCATCTGGACCAACTGGCGGCGCGGGATTTCATCCGC
ATCAAGAAAGCCCTGAACTGTTCCGACGACGAGTTGCGGGACGCCCAGGCCCTGATCCTCACCCTGAACCCCCGGCCCGG
CGCCCAGTACGCGGCCCTGGATACCCGCTACATCGTGCCCGATGTGGTGGTGCGCAAGCTGCGTGGTCAGTGGCTGGCCA
GCCTCAACGGCGAGGCCATGCCCCGGCTGCGCATCAACCGGCTCTATGCCAATATCCTGCGCCAGAACCGGGGCAGCAGC
CTGGCAGGCCACCTGCAGGAAGCCAAGTGGCTGATCAAGAACGTGCAGCAGCGTTTCGACACCATCCTGCGGGTGACCCA
GGCCATCGTGGATCGACAGCGGGCTTTTTTCGACCATGGCGAAGTGGCCATGCGGCCCCTGACCCTGCGGGAAATCGCCG
ACACGGTGGGACTGCACGAATCCACCATCTCCCGGGTGACCACCCAGAAATACCTGGCCAGTCCCCGGGGCATTTTTGAA
CTCAAATACTTTTTTGGCAGCCACGTCGCCACCGATAGCGGCGGCGCGGCCTCCAGTACCGCGATCCGGGCGCTGATCAA
GCAGTTGGTCGGCGCCGAGAACGGCAAGAAGCCGCTCTCGGACAGCCGGCTCGCGGAAATCCTCGGCGAGCAGGGTATTG
TGGTGGCGCGGCGTACCGTCGCCAAGTACCGCGAATCCCTGAACATCCCGCCGGTCAACCTGAGAAAGACGCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(323-476)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(115-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S6Y0W3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.48

100

0.611

Multiple sequence alignment