Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M395_RS12300 Genome accession   NZ_CP006030
Coordinates   2543030..2544400 (-) Length   456 a.a.
NCBI ID   WP_002293096.1    Uniprot ID   A0A4Y3JR63
Organism   Enterococcus faecium T110     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2538030..2549400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M395_RS12285 (M395_12465) epsC 2539350..2539886 (-) 537 WP_002293094.1 serine O-acetyltransferase EpsC -
  M395_RS12290 (M395_12470) gltX 2540209..2541669 (-) 1461 WP_002293095.1 glutamate--tRNA ligase -
  M395_RS12295 (M395_12475) - 2541839..2542996 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  M395_RS12300 (M395_12480) radA 2543030..2544400 (-) 1371 WP_002293096.1 DNA repair protein RadA Machinery gene
  M395_RS12305 (M395_12485) - 2544463..2544990 (-) 528 WP_002373359.1 dUTP diphosphatase -
  M395_RS12310 (M395_12490) proB 2545220..2546026 (+) 807 WP_010733122.1 glutamate 5-kinase -
  M395_RS12315 (M395_12495) - 2546023..2547270 (+) 1248 WP_002373354.1 glutamate-5-semialdehyde dehydrogenase -
  M395_RS12320 (M395_12500) - 2547346..2547690 (+) 345 WP_002293100.1 hypothetical protein -
  M395_RS12325 (M395_12505) - 2547945..2548271 (-) 327 WP_002373352.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49935.60 Da        Isoelectric Point: 7.7416

>NTDB_id=112933 M395_RS12300 WP_002293096.1 2543030..2544400(-) (radA) [Enterococcus faecium T110]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRVKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=112933 M395_RS12300 WP_002293096.1 2543030..2544400(-) (radA) [Enterococcus faecium T110]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGGCGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACAGACCGCAGAACGCGGACAAGTTTGACAGGAG
AAAAAGCGAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGGTCAAAACGAAATTAGAAGAACTGAAC
CGTGTGCTAGGTGGAGGTGTGGTACCGGGATCCATGGTCTTGATCGGTGGTGATCCAGGTATTGGTAAATCCACTTTGCT
ATTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGGAAAGTCTTGTATGTATCCGGCGAAGAAAGCGCAGAACAGATAA
AATTACGCGCGGAGCGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAATGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATTATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGTGTCAGCCAAGTAAGAGAAACCACTGCAGAATTACTCAAAATCGCTAAAACGAATGGGATTGCCATCTTTATCG
TCGGTCATGTTACGAAAGAAGGTTCGATCGCCGGTCCAAGAATGTTAGAGCATATGGTGGATACAGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTCCGAATTTTACGAGCGGTAAAAAACCGATTTGGCTCAACGAACGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAACCCTTCACAGATTTTCTTAGAGGAACGCCTAGACGGTGCGACTGGCT
CTGCTATCGTTGTGGCAATGGAAGGAACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCTAAGCGAACGACAACAGGTCTTGATTTCAATCGTGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGACT
ACTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTACAAAGAAAAAGGAACACAGCCGACGGAATGTTTCATCGGTGAAATCGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGGGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTTTACCTGCCTAA
AAACAATTTAGGCAACTGGGAAGCGCCAAAAGGAATCGAGATCGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(70-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3JR63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus pneumoniae Rx1

74.286

99.781

0.741
  radA Streptococcus pneumoniae D39

74.286

99.781

0.741
  radA Streptococcus pneumoniae R6

74.286

99.781

0.741
  radA Streptococcus pneumoniae TIGR4

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

67.627

98.904

0.669

Multiple sequence alignment