Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QOY30_RS02185 Genome accession   NZ_LR699166
Coordinates   438230..439669 (-) Length   479 a.a.
NCBI ID   WP_283743000.1    Uniprot ID   -
Organism   Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 422646..443097 438230..439669 within 0


Gene organization within MGE regions


Location: 422646..443097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOY30_RS02080 - 422646..422897 (-) 252 WP_283742981.1 helix-turn-helix transcriptional regulator -
  QOY30_RS02085 - 423049..423471 (+) 423 WP_283742982.1 hypothetical protein -
  QOY30_RS02090 (SIDCL21_0432) - 423837..424352 (-) 516 WP_283742983.1 3'-5' exoribonuclease -
  QOY30_RS02095 (SIDCL21_0433) - 424354..424854 (-) 501 WP_283742984.1 HAD domain-containing protein -
  QOY30_RS02100 (SIDCL21_0434) - 425081..425491 (-) 411 WP_283742985.1 hypothetical protein -
  QOY30_RS02105 (SIDCL21_0435) - 425502..425936 (-) 435 WP_283742986.1 hypothetical protein -
  QOY30_RS02110 (SIDCL21_0436) - 425940..426572 (-) 633 WP_283742987.1 metallophosphoesterase family protein -
  QOY30_RS02115 (SIDCL21_0437) - 426569..426979 (-) 411 WP_283742988.1 hypothetical protein -
  QOY30_RS02120 (SIDCL21_0438) - 427005..427757 (-) 753 WP_283742989.1 metallophosphoesterase -
  QOY30_RS02125 (SIDCL21_0439) - 427772..429103 (-) 1332 WP_283742990.1 AAA family ATPase -
  QOY30_RS02130 (SIDCL21_0441) - 429633..429776 (+) 144 WP_349496672.1 HU family DNA-binding protein -
  QOY30_RS02135 (SIDCL21_0442) - 430217..431209 (-) 993 WP_283742991.1 hypothetical protein -
  QOY30_RS02140 (SIDCL21_0443) - 431640..432674 (+) 1035 WP_283742992.1 site-specific integrase -
  QOY30_RS02145 (SIDCL21_0444) - 432971..433681 (+) 711 WP_283742993.1 hypothetical protein -
  QOY30_RS02150 (SIDCL21_0445) - 434017..434616 (-) 600 WP_283742994.1 flavin prenyltransferase UbiX -
  QOY30_RS02155 (SIDCL21_0446) - 434613..435095 (-) 483 WP_283746010.1 Gx transporter family protein -
  QOY30_RS02160 (SIDCL21_0447) - 435214..435582 (-) 369 WP_283742995.1 NusG domain II-containing protein -
  QOY30_RS02165 (SIDCL21_0448) rapZ 435585..436430 (-) 846 WP_283742996.1 RNase adapter RapZ -
  QOY30_RS02170 (SIDCL21_0449) hprK 436432..437367 (-) 936 WP_283742997.1 HPr(Ser) kinase/phosphatase -
  QOY30_RS02175 (SIDCL21_0450) - 437378..437833 (-) 456 WP_283742998.1 PTS sugar transporter subunit IIA -
  QOY30_RS02180 (SIDCL21_0451) raiA 437890..438213 (-) 324 WP_283742999.1 ribosome-associated translation inhibitor RaiA -
  QOY30_RS02185 (SIDCL21_0452) rpoN/rpoN1 438230..439669 (-) 1440 WP_283743000.1 RNA polymerase factor sigma-54 Machinery gene
  QOY30_RS02190 (SIDCL21_0453) lptB 439707..440429 (-) 723 WP_283743001.1 LPS export ABC transporter ATP-binding protein -
  QOY30_RS02195 (SIDCL21_0454) lptA 440426..441025 (-) 600 WP_283743002.1 lipopolysaccharide transport periplasmic protein LptA -
  QOY30_RS02200 (SIDCL21_0455) lptC 441006..441593 (-) 588 WP_283743003.1 LPS export ABC transporter periplasmic protein LptC -
  QOY30_RS02205 (SIDCL21_0456) - 441593..442114 (-) 522 WP_283743004.1 HAD family hydrolase -
  QOY30_RS02210 (SIDCL21_0457) - 442111..443097 (-) 987 WP_283743005.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53896.96 Da        Isoelectric Point: 4.6896

>NTDB_id=1129316 QOY30_RS02185 WP_283743000.1 438230..439669(-) (rpoN/rpoN1) [Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21]
MKHSLQLRQSQQLMLTPQLQQAIKLLQLSTLEINQETARLLDENPFLEREDDSTNQTYSGNSSSDIPAPSGSTETSSAPA
EAESRKDDGNEWPEPTFSSSSSSSGPDDEDDGYSEVAAEKPSMREHLLWELNMSQMDPRDKKIIGLLIDALDENAYLSQP
LEEIFELLPPELDISLDDLETALVQLQYLDAPGIGARNLSECLALQLRAMPEDIPGRDLALAIVSDHLDLLAARDFSKLK
KALRCDDDALRCAQDLIVHLQPKPGSAFEHRAADYVVPDVLVERHGGIWRARLNPEAMPRLRINQVYANILQQRNEKNAQ
EMAGQLQEARWFIKNLQQRFETILRVSQAIVERQRQFFEHGDIAMRPLVLREIAEQLELHESTISRVTTQKFMLTPRGIY
EFKYFFGSGLATEAGGACSSTAIRALIKQLVSEEDGKHPLTDSRMSEILAQQGILVARRTVAKYREGMNILPVNLRKSL

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1129316 QOY30_RS02185 WP_283743000.1 438230..439669(-) (rpoN/rpoN1) [Sideroxydans sp. CL21 isolate Sideroxydans sp. CL21]
ATGAAACACTCTCTCCAACTCAGGCAATCCCAACAACTGATGCTCACGCCCCAGTTGCAGCAGGCCATCAAGCTGCTGCA
ACTTTCGACGCTGGAGATCAATCAGGAGACTGCGCGCCTGCTGGACGAGAATCCCTTCCTTGAGCGCGAGGACGACAGTA
CCAACCAGACCTATAGCGGCAACAGCAGCTCCGACATCCCCGCCCCTTCCGGCAGTACCGAAACGAGCAGCGCCCCTGCC
GAAGCTGAATCACGCAAAGACGACGGCAACGAATGGCCCGAACCTACGTTCTCTTCATCGTCATCCTCGTCCGGCCCTGA
CGACGAGGACGACGGCTATTCCGAAGTCGCCGCCGAGAAACCCAGCATGCGTGAGCACTTGCTCTGGGAATTGAACATGA
GCCAGATGGATCCGCGCGACAAGAAGATCATCGGACTACTCATCGACGCGCTGGACGAAAATGCCTACCTGTCGCAACCG
TTGGAAGAAATTTTCGAACTGCTGCCGCCCGAACTGGACATTTCGCTGGACGATCTGGAAACGGCTCTGGTGCAGTTGCA
ATATCTGGATGCACCCGGTATCGGCGCACGCAACCTGAGCGAATGCCTGGCATTGCAACTGCGTGCCATGCCGGAAGATA
TACCGGGCCGCGATCTGGCATTGGCCATTGTCAGCGATCATCTGGACTTGCTGGCCGCGCGGGATTTCAGCAAATTGAAA
AAGGCCCTGCGCTGCGACGATGACGCCTTGCGCTGCGCACAGGATCTGATCGTGCATCTGCAACCCAAACCCGGTTCGGC
CTTCGAGCATCGTGCTGCGGACTATGTGGTGCCGGACGTACTGGTGGAACGCCACGGCGGCATCTGGCGCGCACGTTTGA
ATCCGGAAGCCATGCCGCGCCTGCGCATCAATCAGGTGTATGCCAACATCCTGCAACAGCGCAACGAGAAGAACGCGCAG
GAAATGGCCGGGCAACTGCAGGAAGCGCGCTGGTTCATCAAAAACCTGCAGCAACGTTTCGAAACTATTTTGCGCGTATC
GCAGGCCATCGTCGAAAGGCAGCGCCAGTTCTTCGAGCACGGTGACATCGCCATGCGCCCGCTGGTGCTGCGCGAGATCG
CCGAACAACTCGAACTGCATGAATCCACCATTTCGCGCGTGACTACACAGAAGTTCATGCTCACCCCGCGCGGCATCTAC
GAATTCAAATACTTCTTCGGCAGCGGCCTTGCCACCGAAGCGGGCGGTGCGTGCTCATCCACCGCCATCCGTGCACTGAT
CAAGCAATTGGTCAGCGAGGAAGACGGGAAACACCCGCTCACCGACAGTCGCATGTCAGAAATCCTGGCACAGCAGGGCA
TTCTAGTCGCCCGCCGTACCGTGGCAAAATACCGCGAGGGGATGAACATCCTTCCGGTAAATCTTCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(321-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(114-307)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.976

100

0.557

Multiple sequence alignment