Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E5P1_RS13250 Genome accession   NZ_LR594689
Coordinates   2686847..2688436 (+) Length   529 a.a.
NCBI ID   WP_068675421.1    Uniprot ID   A0A2J7VSM2
Organism   Variovorax sp. WDL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2681847..2693436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P1_RS13225 (G3W93_RS13250) argS 2682013..2683698 (+) 1686 WP_068675411.1 arginine--tRNA ligase -
  E5P1_RS13230 (G3W93_RS13255) - 2683713..2684426 (+) 714 WP_068675413.1 SPOR domain-containing protein -
  E5P1_RS13235 (G3W93_RS13260) - 2684513..2685157 (+) 645 WP_068675414.1 thiol:disulfide interchange protein DsbA/DsbL -
  E5P1_RS13240 (G3W93_RS13265) lptA 2685412..2686035 (+) 624 WP_232081171.1 lipopolysaccharide transport periplasmic protein LptA -
  E5P1_RS13245 (G3W93_RS13270) lptB 2686050..2686832 (+) 783 WP_068675418.1 LPS export ABC transporter ATP-binding protein -
  E5P1_RS13250 (G3W93_RS13275) rpoN/rpoN1 2686847..2688436 (+) 1590 WP_068675421.1 RNA polymerase factor sigma-54 Machinery gene
  E5P1_RS13255 (G3W93_RS13280) - 2688439..2690043 (+) 1605 WP_068675423.1 phospholipase D family protein -
  E5P1_RS13260 (G3W93_RS13285) - 2690114..2690743 (-) 630 WP_068675496.1 YceI family protein -
  E5P1_RS13265 (G3W93_RS13290) - 2690790..2691380 (-) 591 WP_068675425.1 YceI family protein -
  E5P1_RS13270 (G3W93_RS13295) - 2691377..2691988 (-) 612 WP_068675427.1 cytochrome b -
  E5P1_RS13275 (G3W93_RS13300) - 2692183..2692452 (-) 270 WP_102905638.1 hypothetical protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58384.47 Da        Isoelectric Point: 4.6158

>NTDB_id=1128265 E5P1_RS13250 WP_068675421.1 2686847..2688436(+) (rpoN/rpoN1) [Variovorax sp. WDL1]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERAADEAPREEFGLESADAPVREDDRQVDSESE
FVAPLAAASADSTPASPADGETAAAAEGPEGEPDWEGDGTVDLAPDDSEWGGDAPARNSGTGDDERPDAAELASSQESLQ
SFLHRQALSLRLEENDRAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPVGVGARSLA
ECLAIQLRAQQDEDETEDEKQVRRTAMAICKQPMELLAKRDFKRLATLTRSNEEEVRAALQLIARLEPKPGRRFANVERN
IVIPDVIVTRIGHGTSARFRVMLNPEVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKNYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPYGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSAEDVKKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1128265 E5P1_RS13250 WP_068675421.1 2686847..2688436(+) (rpoN/rpoN1) [Variovorax sp. WDL1]
ATGAAGCAAGGGCTCTCGCTGCGCGTCTCGCAGCACCTCGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAGTTGAGCCAGGAGGTCGAGCAGATGCTCGACGAGAACCCTTTCCTCGAGCGCGCGGCTGACGAGG
CGCCGCGCGAGGAGTTCGGGCTCGAGTCGGCCGATGCGCCGGTGCGCGAAGATGACCGCCAGGTCGACAGCGAGTCCGAG
TTCGTCGCGCCCCTCGCTGCCGCGTCCGCCGACAGTACGCCGGCCTCCCCTGCCGACGGCGAGACAGCCGCGGCCGCCGA
AGGCCCGGAGGGCGAGCCCGACTGGGAAGGCGACGGCACGGTCGACCTCGCACCGGACGACAGCGAATGGGGCGGGGACG
CGCCCGCGCGCAACAGCGGCACCGGCGACGACGAGCGCCCGGATGCGGCCGAGCTCGCCAGCAGCCAGGAGTCGCTGCAG
TCCTTCCTGCATCGCCAGGCGCTGAGCCTGCGCCTGGAGGAGAACGACCGCGCCGCGCTGCGCTTCCTGATCGAGTCGCT
CAACGACGACGGTTACCTCGAGGACTCCCTGCCGGCCCTGGCTTCCGGACTGGCCGGCGACGACAACGACCAGTTCGACG
AGCTGGTTCACCACTTCCAGGTCGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGTCGGCGTCGGTGCGCGCAGCCTGGCC
GAGTGCCTCGCCATCCAGCTGCGCGCGCAGCAGGACGAGGACGAGACCGAGGACGAGAAGCAGGTGCGCCGAACCGCGAT
GGCGATCTGCAAGCAGCCGATGGAGCTTCTCGCCAAGCGCGACTTCAAGCGCCTGGCCACGCTCACCCGCAGCAACGAGG
AGGAAGTGCGCGCCGCCCTGCAGCTGATTGCGCGGCTCGAGCCCAAGCCCGGGCGCCGCTTCGCCAACGTCGAGCGCAAC
ATCGTCATCCCCGACGTGATCGTCACGCGCATCGGCCACGGCACCAGCGCCAGGTTCCGCGTCATGCTCAACCCCGAGGT
GATGCCGCGGCTGCGCGTGCACGACATCTACGCCGGCGCGCTGAAGGCGCACAAGGGCGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCGAACGCGATC
GTGGAGCGGCAGAAGAACTACTTCGTCCACGGCGAGCTCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAATCCACCATCAGCCGCGTGACCACCGCCAAGTACATGTCCACGCCCTACGGCACGGTCGAGCTCAAGT
ACTTCTTCGGCTCGGCGCTGGGGACCGAGACCGGCGGCAACGCCTCCAGCACCGCGGTGCGCGCGCTCATCAAGCAGTTC
GTGAGCGCCGAGGACGTGAAGAAGCCGCTGTCGGACAGCCAGGTTTCCGAGATGCTCAAGGAACAGGGCATCGAGTGCGC
ACGCCGCACGGTGGCCAAGTACCGCGAGGCGCTACGCATCGCGCCCGCCAACCTGCGCAAGGCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J7VSM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.509

100

0.516

Multiple sequence alignment