Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E5CHR_RS27975 Genome accession   NZ_LR594675
Coordinates   5877100..5878689 (+) Length   529 a.a.
NCBI ID   WP_162583042.1    Uniprot ID   A0A6P2FPU8
Organism   Variovorax sp. PBS-H4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5872100..5883689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5CHR_RS27950 (H4CHR_05580) argS 5872255..5873940 (+) 1686 WP_162583037.1 arginine--tRNA ligase -
  E5CHR_RS27955 (H4CHR_05581) - 5873955..5874686 (+) 732 WP_162583038.1 SPOR domain-containing protein -
  E5CHR_RS27960 (H4CHR_05582) - 5874772..5875416 (+) 645 WP_162583039.1 thiol:disulfide interchange protein DsbA/DsbL -
  E5CHR_RS27965 (H4CHR_05583) lptA 5875602..5876288 (+) 687 WP_162583040.1 lipopolysaccharide transport periplasmic protein LptA -
  E5CHR_RS27970 (H4CHR_05584) lptB 5876303..5877085 (+) 783 WP_162583041.1 LPS export ABC transporter ATP-binding protein -
  E5CHR_RS27975 (H4CHR_05585) rpoN/rpoN1 5877100..5878689 (+) 1590 WP_162583042.1 RNA polymerase factor sigma-54 Machinery gene
  E5CHR_RS27980 (H4CHR_05586) - 5878692..5880296 (+) 1605 WP_232062220.1 phospholipase D family protein -
  E5CHR_RS27985 (H4CHR_05587) - 5880360..5881028 (-) 669 WP_232062221.1 YceI family protein -
  E5CHR_RS27990 (H4CHR_05588) - 5881038..5881556 (-) 519 WP_232062328.1 YceI family protein -
  E5CHR_RS27995 (H4CHR_05589) - 5881622..5882233 (-) 612 WP_162583044.1 cytochrome b -
  E5CHR_RS28000 (H4CHR_05591) - 5882433..5882702 (-) 270 WP_162583045.1 hypothetical protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58456.56 Da        Isoelectric Point: 4.6440

>NTDB_id=1128254 E5CHR_RS27975 WP_162583042.1 5877100..5878689(+) (rpoN/rpoN1) [Variovorax sp. PBS-H4]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTADEAPREEFGLESADTPVREDDRQAEGESE
FIAGPAPAPIDSAPTSAADSETAATAESAEGEPDWEGDGTVDLVPDDSEWGGDAPARNSGTADGERPDATELASSQESLQ
SFLHRQALALRLEENDRAALRFLIESLNDDGYLEDSLPSLASGLAGDDNDQFDELVHHFQVALGLLQSLEPVGVGARSLA
ECLALQLRAQQEEDDTEEDRQVRRTAIAVCKQPMELLAKRDFKRLATLTRSNEEEVRAALQLIARLEPKPGRRFANVERN
IVIPDVIVTRIGHGTSAKFRVMLNPEVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKNYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPYGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSAEDVKKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1128254 E5CHR_RS27975 WP_162583042.1 5877100..5878689(+) (rpoN/rpoN1) [Variovorax sp. PBS-H4]
ATGAAGCAAGGGCTCTCGCTGCGTGTCTCGCAGCACCTGGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGCCTGCTGCA
GCTGTCCACGCTCGAGCTGAGCCAGGAGGTCGAGCAGATGCTCGACGAGAACCCTTTCCTGGAGCGAACCGCCGATGAAG
CCCCGCGCGAGGAGTTCGGGCTGGAATCCGCCGACACACCGGTGCGCGAGGATGACCGCCAAGCCGAGGGGGAGAGCGAG
TTCATCGCGGGTCCGGCCCCGGCTCCGATCGACAGTGCTCCGACCTCGGCAGCCGACAGCGAGACCGCCGCCACTGCCGA
AAGCGCGGAGGGCGAGCCCGACTGGGAAGGCGACGGCACGGTCGACCTGGTGCCGGACGACAGCGAATGGGGCGGCGACG
CGCCCGCGCGCAACAGCGGCACGGCCGACGGCGAGCGTCCCGATGCGACCGAGCTCGCCAGCAGCCAGGAGTCGCTGCAG
TCCTTCCTGCACCGCCAGGCGCTGGCCTTGCGCCTGGAGGAGAACGACCGCGCCGCGCTGCGCTTCCTGATCGAATCGCT
CAACGACGATGGCTACCTCGAGGACTCGCTGCCGTCTTTGGCCTCGGGCTTGGCCGGCGACGACAACGACCAGTTCGACG
AACTGGTGCACCACTTCCAGGTCGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGTCGGCGTCGGTGCGCGCAGCCTGGCA
GAGTGTCTCGCGCTCCAGCTGCGCGCGCAGCAGGAAGAAGACGACACCGAGGAAGACAGGCAGGTGCGCCGGACGGCGAT
TGCGGTCTGCAAGCAGCCGATGGAGCTGCTCGCCAAGCGCGACTTCAAGCGCCTTGCCACGCTCACCCGCAGCAACGAAG
AGGAAGTGCGCGCCGCCCTGCAGCTGATCGCACGGCTGGAGCCGAAGCCGGGGCGCCGCTTCGCCAATGTCGAGCGCAAC
ATCGTCATCCCCGACGTGATCGTCACGCGCATCGGCCACGGCACCAGCGCCAAGTTCCGCGTCATGCTCAACCCCGAGGT
GATGCCGCGGTTGCGCGTGCACGACATCTACGCCGGCGCGCTGAAGGCGCACAAGGGCGAAGGCAGCCAGGCGCTCTCGC
AACGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAACGCGATC
GTCGAGCGGCAGAAGAACTACTTCGTCCACGGCGAGCTCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAATCCACCATCAGCCGCGTGACGACCGCCAAGTACATGTCCACGCCTTATGGCACGGTCGAGCTCAAGT
ACTTCTTCGGTTCGGCGCTGGGCACCGAGACCGGCGGAAATGCCTCCAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTC
GTGAGCGCCGAGGACGTGAAAAAGCCGCTATCGGACAGCCAGGTCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTGTGC
GCGCCGCACCGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCGGCCAACCTGCGCAAGGCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2FPU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.06

100

0.526

Multiple sequence alignment