Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WDLP6_RS25335 Genome accession   NZ_LR594671
Coordinates   5198961..5200559 (-) Length   532 a.a.
NCBI ID   WP_162594582.1    Uniprot ID   A0A6P2FKP8
Organism   Variovorax sp. PBL-E5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5193961..5205559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDLP6_RS25315 (E5CHR_05053) - 5195406..5195993 (+) 588 WP_162594579.1 cytochrome b -
  WDLP6_RS25320 (E5CHR_05054) - 5195990..5196676 (+) 687 WP_162594580.1 YceI family protein -
  WDLP6_RS25325 (E5CHR_05055) - 5196721..5197338 (+) 618 WP_162595254.1 YceI family protein -
  WDLP6_RS25330 (E5CHR_05056) - 5197351..5198949 (-) 1599 WP_162594581.1 phospholipase D family protein -
  WDLP6_RS25335 (E5CHR_05057) rpoN/rpoN1 5198961..5200559 (-) 1599 WP_162594582.1 RNA polymerase factor sigma-54 Machinery gene
  WDLP6_RS25340 (E5CHR_05058) lptB 5200573..5201367 (-) 795 WP_162569896.1 LPS export ABC transporter ATP-binding protein -
  WDLP6_RS25345 (E5CHR_05059) lptA 5201364..5202071 (-) 708 WP_162594583.1 lipopolysaccharide transport periplasmic protein LptA -
  WDLP6_RS25350 (E5CHR_05060) - 5202177..5202824 (-) 648 WP_162594584.1 thiol:disulfide interchange protein DsbA/DsbL -
  WDLP6_RS25355 (E5CHR_05061) - 5202908..5203609 (-) 702 WP_162594585.1 SPOR domain-containing protein -
  WDLP6_RS25360 (E5CHR_05062) argS 5203626..5205299 (-) 1674 WP_162594586.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58272.47 Da        Isoelectric Point: 4.7414

>NTDB_id=1128236 WDLP6_RS25335 WP_162594582.1 5198961..5200559(-) (rpoN/rpoN1) [Variovorax sp. PBL-E5]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLERTAEEAQREEFGLDAADAPVRDDDRLAEGDGE
LPAGPSAPLADAGVGSTSTAAEADAPATADSTDGEPGWEGDGTVDVAPDDSEWGGDAPARANNLGDDERADATELARSQE
SLQSFLHRQALGLRLDDNDRAALRFLIESLNDDGYLEDSLPALASGLAGEDNDQFDELVHHFQVALGLLQSLEPVGVGAR
SLGECLTIQLRALAHANEGDQEAQIRKTAIAVCKQPMELLARRDFKRLATLTRSSEDTVRAALQVIARLEPKPGRRFVDV
ERNVVVPDVIVTRIGQGTNVKFRVMLNPEVMPRLRVHDIYAGALKQHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVS
NAIVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPYGTVELKYFFGSALGTETGGNASSTAVRALI
KQFVSAEDTRKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=1128236 WDLP6_RS25335 WP_162594582.1 5198961..5200559(-) (rpoN/rpoN1) [Variovorax sp. PBL-E5]
ATGAAGCAGGGTCTCTCGCTTCGAGTCTCTCAGCATCTCGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAGCTGAGCCAGGAAGTCGAGCAGATGCTCGACGACAACCCGTTCCTCGAACGCACCGCCGAAGAAG
CGCAGCGCGAGGAGTTCGGGCTCGACGCGGCCGATGCACCGGTGCGCGACGACGATCGCCTGGCCGAGGGCGACGGCGAG
CTGCCGGCCGGCCCGTCCGCGCCGCTGGCTGACGCCGGCGTCGGCAGCACGAGCACCGCGGCCGAAGCCGACGCGCCGGC
CACGGCTGACAGCACGGATGGGGAGCCCGGCTGGGAAGGCGATGGCACCGTCGATGTGGCACCCGACGACAGCGAGTGGG
GCGGCGACGCGCCCGCGCGCGCCAACAACCTCGGCGACGACGAGCGTGCCGATGCCACCGAACTGGCGCGCAGCCAGGAG
TCGCTGCAATCTTTCCTGCACCGCCAGGCACTGGGCCTGCGGCTGGACGACAACGACCGCGCCGCGCTGCGCTTCCTGAT
CGAGTCGCTCAACGACGACGGCTACCTCGAGGACTCGCTGCCGGCGCTCGCCTCCGGCCTCGCGGGCGAAGACAACGACC
AGTTCGACGAGCTGGTGCACCACTTCCAGGTCGCGCTCGGTCTGCTGCAGAGCCTGGAGCCGGTCGGCGTCGGCGCCCGC
AGCCTCGGCGAATGCCTCACGATCCAGCTGCGCGCGCTGGCGCATGCGAACGAGGGCGACCAGGAGGCGCAGATCCGCAA
GACCGCGATCGCCGTCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGATTTCAAGCGCCTGGCCACGCTCACCCGCA
GCAGCGAAGACACGGTGCGTGCCGCGCTGCAGGTGATCGCCAGGCTCGAGCCCAAGCCGGGGCGCCGCTTCGTCGATGTC
GAGCGCAACGTCGTCGTGCCCGACGTGATCGTCACGCGCATCGGCCAGGGCACGAACGTCAAGTTCCGCGTCATGCTCAA
TCCCGAGGTCATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGCAGCACAAGGGCGAGGGCAGCCAGG
CGCTGTCGCAGCGCTTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTTCGCGTGTCC
AACGCGATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCATGGCGAGCTCGCGATGCGGCCGCTGGTGCTGCGCGAGATCGC
CGACGAGCTCGGCCTGCACGAGTCGACCATCAGCCGCGTGACCACCGCCAAGTACATGGCGACGCCCTATGGCACCGTCG
AGCTCAAGTACTTCTTCGGCTCGGCACTCGGCACCGAGACCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTGATC
AAGCAGTTCGTGAGCGCCGAGGACACGAGAAAGCCGCTGTCGGACAGCCAGGTCTCCGAGATGCTCAAGGAGCAGGGCAT
CGAATGCGCGCGCCGGACCGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCGGCCAATCTGCGCAAGGCCCTCTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-530)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(153-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2FKP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.487

100

0.521

Multiple sequence alignment