Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E5P2_RS25580 Genome accession   NZ_LR594666
Coordinates   5296501..5298099 (-) Length   532 a.a.
NCBI ID   WP_162569895.1    Uniprot ID   A0A8B6LT62
Organism   Variovorax sp. SRS16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5291501..5303099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P2_RS25560 (G3W92_RS25540) - 5292946..5293533 (+) 588 WP_162569893.1 cytochrome b -
  E5P2_RS25565 (G3W92_RS25545) - 5293716..5294216 (+) 501 WP_162570664.1 YceI family protein -
  E5P2_RS25570 (G3W92_RS25550) - 5294261..5294878 (+) 618 WP_162570665.1 YceI family protein -
  E5P2_RS25575 (G3W92_RS25555) - 5294891..5296489 (-) 1599 WP_162569894.1 phospholipase D family protein -
  E5P2_RS25580 (G3W92_RS25560) rpoN/rpoN1 5296501..5298099 (-) 1599 WP_162569895.1 RNA polymerase factor sigma-54 Machinery gene
  E5P2_RS25585 (G3W92_RS25565) lptB 5298113..5298907 (-) 795 WP_162569896.1 LPS export ABC transporter ATP-binding protein -
  E5P2_RS25590 (G3W92_RS25570) lptA 5298904..5299611 (-) 708 WP_162569897.1 lipopolysaccharide transport periplasmic protein LptA -
  E5P2_RS25595 (G3W92_RS25575) - 5299717..5300364 (-) 648 WP_162569898.1 thiol:disulfide interchange protein DsbA/DsbL -
  E5P2_RS25600 (G3W92_RS25580) - 5300448..5301149 (-) 702 WP_162569899.1 SPOR domain-containing protein -
  E5P2_RS25605 (G3W92_RS25585) argS 5301166..5302839 (-) 1674 WP_162569900.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58283.53 Da        Isoelectric Point: 4.7767

>NTDB_id=1128215 E5P2_RS25580 WP_162569895.1 5296501..5298099(-) (rpoN/rpoN1) [Variovorax sp. SRS16]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLERTAEEAQREEFGLDAADAPVRDDDRLAEGDGE
LPSGPSAPLADAGVGSTSTAAEADAPATADSTDGEPGWEGDGTVDVAPDDSEWGGDAPARANNLGNDERADATELARSQE
SLQSFLHRQALGLRLDDNDRAALRFLIESLNDDGYLEDSLPALASGLAGEDNDQFDELVHHFQVALGLLQSLEPVGVGAR
SLGECLTIQLRALAHANEGDQEAQIRKIAIAVCKQPMELLARRDFKRLATLTRSSEDTVRAALQVIARLEPKPGRRFVDV
ERNVVVPDVIVTRIGQGTNVKFRVMLNPEVMPRLRVHDIYAGALKQHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVS
NAIVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTVELKYFFGSALGTETGGNASSTAVRALI
KQFVSAEDTRKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=1128215 E5P2_RS25580 WP_162569895.1 5296501..5298099(-) (rpoN/rpoN1) [Variovorax sp. SRS16]
ATGAAGCAGGGTCTCTCGCTTCGAGTCTCTCAGCATCTCGCGCTCACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAGCTGAGCCAGGAAGTCGAGCAGATGCTCGACGACAACCCGTTCCTCGAACGCACCGCCGAAGAAG
CGCAGCGCGAGGAGTTCGGGCTCGACGCGGCCGATGCACCGGTGCGCGACGACGATCGCCTGGCCGAGGGCGACGGCGAG
CTGCCGTCCGGCCCGTCCGCGCCGCTGGCTGACGCCGGCGTCGGCAGCACGAGCACCGCGGCCGAAGCCGACGCGCCGGC
CACGGCCGACAGCACGGATGGGGAGCCCGGCTGGGAAGGCGATGGCACCGTCGATGTGGCACCCGACGACAGCGAGTGGG
GCGGCGACGCGCCCGCGCGCGCCAACAACCTCGGCAACGACGAGCGTGCCGATGCCACCGAACTGGCGCGCAGCCAGGAG
TCGCTGCAATCTTTCCTGCACCGCCAGGCATTGGGCCTGCGGCTGGACGACAACGACCGCGCCGCGCTGCGCTTCCTGAT
CGAGTCGCTCAACGACGACGGCTACCTCGAGGACTCGCTGCCGGCGCTCGCCTCCGGCCTCGCGGGCGAAGACAACGACC
AGTTCGACGAGCTGGTGCACCACTTCCAGGTCGCGCTCGGTCTGCTGCAGAGCCTGGAGCCAGTCGGCGTCGGCGCCCGC
AGCCTCGGCGAATGCCTCACGATCCAGCTGCGCGCGCTGGCGCATGCGAACGAGGGCGACCAGGAGGCGCAGATCCGCAA
GATCGCGATCGCCGTCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGATTTCAAGCGCCTGGCCACGCTCACCCGCA
GCAGCGAAGACACGGTGCGTGCCGCGCTGCAGGTGATCGCCAGGCTCGAGCCCAAGCCGGGGCGCCGCTTCGTCGATGTC
GAGCGCAACGTCGTCGTGCCCGACGTGATCGTCACGCGCATCGGCCAAGGCACGAACGTCAAGTTCCGCGTCATGCTCAA
TCCCGAGGTCATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGCAGCACAAGGGCGAGGGCAGCCAGG
CGCTGTCGCAGCGCTTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTTCGCGTGTCC
AACGCGATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCATGGCGAGCTCGCGATGCGTCCGCTGGTGCTGCGCGAGATCGC
CGACGAGCTCGGCCTGCACGAGTCGACCATCAGCCGCGTGACCACCGCCAAGTACATGGCGACGCCCTTTGGCACCGTCG
AGCTCAAGTACTTCTTCGGCTCGGCACTCGGCACCGAGACCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTGATC
AAGCAGTTCGTGAGCGCCGAGGACACGAGAAAGCCGCTGTCGGACAGCCAGGTCTCCGAGATGCTCAAGGAGCAGGGCAT
CGAATGCGCGCGCCGGACCGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCGGCCAATCTGCGCAAGGCCCTCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(153-360)

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-530)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B6LT62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.032

100

0.511

Multiple sequence alignment