Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E5P3_RS27990 Genome accession   NZ_LR594662
Coordinates   5875497..5877086 (+) Length   529 a.a.
NCBI ID   WP_162588933.1    Uniprot ID   A0A6P2FDT3
Organism   Variovorax sp. RA8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5870497..5882086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P3_RS27965 (G3W91_RS27970) argS 5870665..5872350 (+) 1686 WP_162588928.1 arginine--tRNA ligase -
  E5P3_RS27970 (G3W91_RS27975) - 5872365..5873072 (+) 708 WP_162588929.1 SPOR domain-containing protein -
  E5P3_RS27975 (G3W91_RS27980) - 5873158..5873802 (+) 645 WP_162588930.1 thiol:disulfide interchange protein DsbA/DsbL -
  E5P3_RS27980 (G3W91_RS27985) lptA 5874062..5874685 (+) 624 WP_232073350.1 lipopolysaccharide transport periplasmic protein LptA -
  E5P3_RS27985 (G3W91_RS27990) lptB 5874700..5875482 (+) 783 WP_162588932.1 LPS export ABC transporter ATP-binding protein -
  E5P3_RS27990 (G3W91_RS27995) rpoN/rpoN1 5875497..5877086 (+) 1590 WP_162588933.1 RNA polymerase factor sigma-54 Machinery gene
  E5P3_RS27995 (G3W91_RS28000) - 5877089..5878693 (+) 1605 WP_162588934.1 phospholipase D family protein -
  E5P3_RS28000 (G3W91_RS28005) - 5878764..5879393 (-) 630 WP_162589912.1 YceI family protein -
  E5P3_RS28005 (G3W91_RS28010) - 5879440..5880030 (-) 591 WP_162588935.1 YceI family protein -
  E5P3_RS28010 (G3W91_RS28015) - 5880027..5880638 (-) 612 WP_162588936.1 cytochrome b/b6 domain-containing protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58442.45 Da        Isoelectric Point: 4.6396

>NTDB_id=1128194 E5P3_RS27990 WP_162588933.1 5875497..5877086(+) (rpoN/rpoN1) [Variovorax sp. RA8]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTADEAPREEFGLDSADAPVRDDDRQVDSESE
FVATPAAASADSAPASPADSETAAAAEGPESEPDWEGDGTVDLAPDDSEWGGDAPARNSGTGDNERPDATELASSQESLQ
SFLHRQALSLRLEENDRAALRFLIESLNDDGYLEDSLPALASGLAGDDNEQFDELVHHFQVALGLLQNLEPVGVGARSLA
ECLAIQLRAQQEEDDTEDEKQVRRTAIAICKQPMELLAKRDFKRLATLTRSNEEEVRAALQLIARLEPKPGRRFANVERN
IVIPDVIVTRVGHGTSARFRVMLNPEVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAI
VERQKNYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPYGTVELKYFFGSALGTETGGNASSTAVRALIKQF
VSAEDVKKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1128194 E5P3_RS27990 WP_162588933.1 5875497..5877086(+) (rpoN/rpoN1) [Variovorax sp. RA8]
ATGAAGCAAGGGCTCTCGCTGCGCGTCTCGCAGCACCTCGCGCTGACGCCGCAATTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAGTTGAGCCAGGAAGTCGAGCAGATGCTCGACGAGAACCCTTTCCTGGAGCGCACGGCCGACGAGG
CGCCGCGCGAAGAGTTCGGGCTCGACTCGGCCGACGCGCCGGTGCGCGACGATGACCGCCAGGTCGACAGCGAGTCCGAG
TTCGTCGCGACCCCCGCTGCGGCGTCCGCCGACAGTGCGCCGGCCTCCCCCGCCGACAGCGAGACAGCCGCGGCCGCCGA
AGGCCCGGAGAGCGAGCCCGACTGGGAAGGCGACGGCACGGTCGACCTCGCGCCGGACGACAGCGAATGGGGCGGCGACG
CGCCCGCGCGCAACAGCGGCACCGGCGACAACGAGCGCCCCGATGCGACCGAACTCGCCAGCAGCCAGGAGTCGCTGCAG
TCCTTCCTGCATCGCCAGGCACTGAGCCTGCGCCTGGAGGAGAACGACCGCGCCGCGCTGCGCTTCCTGATCGAGTCGCT
CAACGACGACGGCTACCTCGAGGACTCGCTGCCGGCCCTGGCCTCCGGCCTGGCCGGCGACGACAACGAACAGTTCGACG
AGCTGGTGCACCACTTCCAGGTCGCGCTCGGCCTGCTGCAGAACCTGGAGCCGGTCGGCGTCGGTGCCCGCAGCCTGGCC
GAGTGCCTCGCCATCCAGCTGCGCGCGCAGCAGGAAGAGGACGACACCGAGGACGAGAAGCAGGTGCGCCGGACCGCGAT
CGCAATCTGCAAGCAGCCGATGGAGCTGCTCGCCAAGCGCGACTTCAAGCGCCTGGCCACGCTCACCCGCAGCAACGAGG
AGGAAGTGCGCGCCGCCCTGCAGCTTATCGCGCGGCTGGAGCCCAAGCCCGGACGCCGCTTCGCCAACGTCGAGCGCAAC
ATCGTCATCCCCGACGTGATCGTCACGCGCGTGGGCCACGGCACCAGCGCCAGGTTCCGCGTCATGCTCAACCCCGAGGT
GATGCCGCGGCTGCGCGTGCACGACATCTACGCCGGCGCGCTGAAGGCGCACAAGGGTGAGGGCAGCCAGGCGCTGTCGC
AGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCGAACGCGATC
GTGGAGCGGCAGAAGAACTACTTCGTCCACGGCGAGCTCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCT
GGGCCTGCACGAATCCACCATCAGCCGCGTGACCACTGCCAAGTACATGTCCACGCCCTACGGCACGGTCGAGCTCAAGT
ACTTCTTCGGCTCGGCGCTGGGCACCGAGACCGGCGGCAACGCCTCCAGCACCGCGGTGCGCGCCCTGATCAAGCAGTTC
GTGAGCGCCGAGGACGTGAAGAAGCCGCTGTCGGACAGCCAGGTTTCCGAGATGCTCAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACGGTGGCCAAGTACCGCGAGGCGCTGCGCATCGCGCCTGCCAACCTGCGCAAGGCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2FDT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.907

100

0.507

Multiple sequence alignment