Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   PSYRMG_RS18040 Genome accession   NZ_CP005970
Coordinates   4115965..4118184 (-) Length   739 a.a.
NCBI ID   WP_003365984.1    Uniprot ID   -
Organism   Pseudomonas syringae UMAF0158     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4110965..4123184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRMG_RS18020 (PSYRMG_18355) hslU 4111293..4112630 (-) 1338 WP_060708558.1 ATP-dependent protease ATPase subunit HslU -
  PSYRMG_RS18025 (PSYRMG_18360) hslV 4112697..4113227 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  PSYRMG_RS18030 (PSYRMG_18365) - 4113434..4114129 (-) 696 WP_003432151.1 SPOR domain-containing protein -
  PSYRMG_RS18035 (PSYRMG_18370) argS 4114131..4115867 (-) 1737 WP_060708559.1 arginine--tRNA ligase -
  PSYRMG_RS18040 (PSYRMG_18375) priA 4115965..4118184 (-) 2220 WP_003365984.1 primosomal protein N' Machinery gene
  PSYRMG_RS18045 (PSYRMG_18380) rpmE 4118354..4118575 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  PSYRMG_RS18050 (PSYRMG_18385) - 4118589..4119392 (+) 804 WP_054081259.1 thermonuclease family protein -
  PSYRMG_RS18055 (PSYRMG_18390) - 4119575..4120843 (+) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81870.19 Da        Isoelectric Point: 8.5462

>NTDB_id=112696 PSYRMG_RS18040 WP_003365984.1 4115965..4118184(-) (priA) [Pseudomonas syringae UMAF0158]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=112696 PSYRMG_RS18040 WP_003365984.1 4115965..4118184(-) (priA) [Pseudomonas syringae UMAF0158]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCAATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCCGCG
CTGTTCAAACTGTGCCTGTGGACCTCGCAGTATTACCAGCACAGCCTGGGCGACACGCTGAGCTGGGCCTTACCGGTGTT
GCTGCGTCAGGGCGAACTGGCCGAAAGCCGCCAGGAACGCTTCTGGCACGTTGCACCGGGTGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAGGCCCTGACCACCCTTGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTCAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTCCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAGACCCTCGAGGCGGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCTGGGCTG
ATCATCATCGACGAGGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCCTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCATAACGCCTACACCG
GCCGCTACGGCCTGCTGCGCCTGAATCAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGTCAGACGCTCGCGGCAGGCCAGCAGGTATTGGT
ATTCCTCAACCGTCGCGGCTTTGCACCCACCTTGCTGTGCCACGACTGCGGGTGGATGTCCGGCTGCCAGCGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGTGTACCGCGCCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCGGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGCCAGCCATGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTATCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTTCGCGCCAGCGAGCGCATGGCGCAACTGATCGTTCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAACCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCACTGCTGATTCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
GGTTGCGCAGATGGCGCTGGGCGGCATTGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGACGTTATC
GCGCGCAGTTGCTGGTGCAGTCCAGCGCCCGTGCGCCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGCCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment